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KIAA1199 as a potential diagnostic biomarker of rheumatoid arthritis related to angiogenesis

Our previous proteomic study on fibroblast-like synoviocytes (FLSs) derived from the synovial tissues found that the expression of KIAA1199 was higher in rheumatoid arthritis (RA) patients than in healthy controls. The aim of this study was to examine the biological function of KIAA1199 and evaluate... Full description

Journal Title: Arthritis research & therapy 29 May 2015, Vol.17, pp.140
Main Author: Yang, Xinyu
Other Authors: Qiu, Pengcheng , Chen, Bingbing , Lin, Yaoyao , Zhou, Zhonghao , Ge, Renshan , Zou, Hai , Wang, Jianmin , Wang, Jianguang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1478-6362 ; PMID: 26022278 Version:1 ; DOI: 10.1186/s13075-015-0637-y
Link: http://pubmed.gov/26022278
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title: KIAA1199 as a potential diagnostic biomarker of rheumatoid arthritis related to angiogenesis
format: Article
creator:
  • Yang, Xinyu
  • Qiu, Pengcheng
  • Chen, Bingbing
  • Lin, Yaoyao
  • Zhou, Zhonghao
  • Ge, Renshan
  • Zou, Hai
  • Wang, Jianmin
  • Wang, Jianguang
subjects:
  • Arthritis, Rheumatoid -- Diagnosis
  • Biomarkers -- Analysis
  • Neovascularization, Pathologic -- Metabolism
  • Proteins -- Metabolism
ispartof: Arthritis research & therapy, 29 May 2015, Vol.17, pp.140
description: Our previous proteomic study on fibroblast-like synoviocytes (FLSs) derived from the synovial tissues found that the expression of KIAA1199 was higher in rheumatoid arthritis (RA) patients than in healthy controls. The aim of this study was to examine the biological function of KIAA1199 and evaluate its clinical diagnosis value in RA. The over-expression of KIAA1199 was verified by quantitative real-time polymerase chain reaction (qPCR), Immunohistochemistry, Immunofluorescence and enzyme linked immunosorbent assay (ELISA) in inactive and active RA patients and healthy controls. The effect of KIAA1199 expression on FLSs proliferation, angiogenesis and related pathway were analyzed by MTT, cell migration, tube formation, chorioallantoic membrane (CAM) assay, qPCR and western-blotting after KIAA1199 knockdown and over-expression. The verification results show the up-regulation of KIAA1199 in RA patients at mRNA and protein level as compared to that in healthy controls. ELISA and receiver operator characteristic (ROC) analysis shows that KIAA1199 concentration in serum, synovial fluid and synovial tissues could be used as dependable biomarkers for the diagnosis of active RA, provided an area under roc curve (AUC) of 0.83, 0.92 and 0.92. Sensitivity and specificity, which were determined by cut-off points, reached 72% 84% and 80% in sensitivity and 80%, 93.3%, 93.3% in specificity, respectively. Moreover, KIAA1199 also enhance the proliferation and angiogenesis of synovial membrane, and KIAA1199/ PLXNB3/ SEMA5A/CTGF axis may be a newly found pathway enhancing cell proliferation and angiogenesis. KIAA1199 may be a potential diagnostic biomarker of RA related to angiogenesis.
language: eng
source:
identifier: E-ISSN: 1478-6362 ; PMID: 26022278 Version:1 ; DOI: 10.1186/s13075-015-0637-y
fulltext: fulltext
issn:
  • 14786362
  • 1478-6362
url: Link


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titleKIAA1199 as a potential diagnostic biomarker of rheumatoid arthritis related to angiogenesis
creatorYang, Xinyu ; Qiu, Pengcheng ; Chen, Bingbing ; Lin, Yaoyao ; Zhou, Zhonghao ; Ge, Renshan ; Zou, Hai ; Wang, Jianmin ; Wang, Jianguang
ispartofArthritis research & therapy, 29 May 2015, Vol.17, pp.140
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subjectArthritis, Rheumatoid -- Diagnosis ; Biomarkers -- Analysis ; Neovascularization, Pathologic -- Metabolism ; Proteins -- Metabolism
descriptionOur previous proteomic study on fibroblast-like synoviocytes (FLSs) derived from the synovial tissues found that the expression of KIAA1199 was higher in rheumatoid arthritis (RA) patients than in healthy controls. The aim of this study was to examine the biological function of KIAA1199 and evaluate its clinical diagnosis value in RA. The over-expression of KIAA1199 was verified by quantitative real-time polymerase chain reaction (qPCR), Immunohistochemistry, Immunofluorescence and enzyme linked immunosorbent assay (ELISA) in inactive and active RA patients and healthy controls. The effect of KIAA1199 expression on FLSs proliferation, angiogenesis and related pathway were analyzed by MTT, cell migration, tube formation, chorioallantoic membrane (CAM) assay, qPCR and western-blotting after KIAA1199 knockdown and over-expression. The verification results show the up-regulation of KIAA1199 in RA patients at mRNA and protein level as compared to that in healthy controls. ELISA and receiver operator characteristic (ROC) analysis shows that KIAA1199 concentration in serum, synovial fluid and synovial tissues could be used as dependable biomarkers for the diagnosis of active RA, provided an area under roc curve (AUC) of 0.83, 0.92 and 0.92. Sensitivity and specificity, which were determined by cut-off points, reached 72% 84% and 80% in sensitivity and 80%, 93.3%, 93.3% in specificity, respectively. Moreover, KIAA1199 also enhance the proliferation and angiogenesis of synovial membrane, and KIAA1199/ PLXNB3/ SEMA5A/CTGF axis may be a newly found pathway enhancing cell proliferation and angiogenesis. KIAA1199 may be a potential diagnostic biomarker of RA related to angiogenesis.
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titleKIAA1199 as a potential diagnostic biomarker of rheumatoid arthritis related to angiogenesis
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0Our previous proteomic study on fibroblast-like synoviocytes (FLSs) derived from the synovial tissues found that the expression of KIAA1199 was higher in rheumatoid arthritis (RA) patients than in healthy controls. The aim of this study was to examine the biological function of KIAA1199 and evaluate its clinical diagnosis value in RA.
1The over-expression of KIAA1199 was verified by quantitative real-time polymerase chain reaction (qPCR), Immunohistochemistry, Immunofluorescence and enzyme linked immunosorbent assay (ELISA) in inactive and active RA patients and healthy controls. The effect of KIAA1199 expression on FLSs proliferation, angiogenesis and related pathway were analyzed by MTT, cell migration, tube formation, chorioallantoic membrane (CAM) assay, qPCR and western-blotting after KIAA1199 knockdown and over-expression.
2The verification results show the up-regulation of KIAA1199 in RA patients at mRNA and protein level as compared to that in healthy controls. ELISA and receiver operator characteristic (ROC) analysis shows that KIAA1199 concentration in serum, synovial fluid and synovial tissues could be used as dependable biomarkers for the diagnosis of active RA, provided an area under roc curve (AUC) of 0.83, 0.92 and 0.92. Sensitivity and specificity, which were determined by cut-off points, reached 72% 84% and 80% in sensitivity and 80%, 93.3%, 93.3% in specificity, respectively. Moreover, KIAA1199 also enhance the proliferation and angiogenesis of synovial membrane, and KIAA1199/ PLXNB3/ SEMA5A/CTGF axis may be a newly found pathway enhancing cell proliferation and angiogenesis.
3KIAA1199 may be a potential diagnostic biomarker of RA related to angiogenesis.
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abstractOur previous proteomic study on fibroblast-like synoviocytes (FLSs) derived from the synovial tissues found that the expression of KIAA1199 was higher in rheumatoid arthritis (RA) patients than in healthy controls. The aim of this study was to examine the biological function of KIAA1199 and evaluate its clinical diagnosis value in RA.
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date2015-05-29