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High throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery

To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines. The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quanti... Full description

Journal Title: Oncotarget 06 October 2015, Vol.6(30), pp.29296-310
Main Author: Wang, Dan
Other Authors: Hu, Kebang , Gao, Ningning , Zhang, Hao , Jiang, Yanlin , Liu, Caigang , Wang, Shouyu , Zhao, Zuowei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1949-2553 ; PMID: 26313265 Version:1 ; DOI: 10.18632/oncotarget.4828
Link: http://pubmed.gov/26313265
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recordid: medline26313265
title: High throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery
format: Article
creator:
  • Wang, Dan
  • Hu, Kebang
  • Gao, Ningning
  • Zhang, Hao
  • Jiang, Yanlin
  • Liu, Caigang
  • Wang, Shouyu
  • Zhao, Zuowei
subjects:
  • Breast Cancer
  • Proliferation
  • Wound Fluid
  • High-Throughput Screening Assays
  • Wound Healing
  • Body Fluids -- Enzymology
  • Breast Neoplasms -- Enzymology
  • Chemokines -- Metabolism
  • Cytokines -- Metabolism
  • Mastectomy -- Adverse Effects
  • Matrix Metalloproteinases -- Metabolism
ispartof: Oncotarget, 06 October 2015, Vol.6(30), pp.29296-310
description: To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines. The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits. The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested from patients underwent NAC showed significant higher profiles of interleukin-1β (IL-1β), IL-4, IL-6, IL-17F, IL-21, IL-23, IL-25, IL-31, Interferon γ (IFNγ), CD40 ligand (CD40L), tumor necrosis factor α (TNFα), CXCL1, CXCL2, CXCL5, CCL3, CCL7 and CCL20. Surgery-induced WF promotes the proliferation and migration of breast cancer cells. The composition of WF is influenced by various clinical features and provides potential therapeutic targets to control local recurrence and tumor progression.
language: eng
source:
identifier: E-ISSN: 1949-2553 ; PMID: 26313265 Version:1 ; DOI: 10.18632/oncotarget.4828
fulltext: fulltext
issn:
  • 19492553
  • 1949-2553
url: Link


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titleHigh throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery
creatorWang, Dan ; Hu, Kebang ; Gao, Ningning ; Zhang, Hao ; Jiang, Yanlin ; Liu, Caigang ; Wang, Shouyu ; Zhao, Zuowei
ispartofOncotarget, 06 October 2015, Vol.6(30), pp.29296-310
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subjectBreast Cancer ; Proliferation ; Wound Fluid ; High-Throughput Screening Assays ; Wound Healing ; Body Fluids -- Enzymology ; Breast Neoplasms -- Enzymology ; Chemokines -- Metabolism ; Cytokines -- Metabolism ; Mastectomy -- Adverse Effects ; Matrix Metalloproteinases -- Metabolism
descriptionTo clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines. The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits. The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested from patients underwent NAC showed significant higher profiles of interleukin-1β (IL-1β), IL-4, IL-6, IL-17F, IL-21, IL-23, IL-25, IL-31, Interferon γ (IFNγ), CD40 ligand (CD40L), tumor necrosis factor α (TNFα), CXCL1, CXCL2, CXCL5, CCL3, CCL7 and CCL20. Surgery-induced WF promotes the proliferation and migration of breast cancer cells. The composition of WF is influenced by various clinical features and provides potential therapeutic targets to control local recurrence and tumor progression.
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titleHigh throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery
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0To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines.
1The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits.
2The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested from patients underwent NAC showed significant higher profiles of interleukin-1β (IL-1β), IL-4, IL-6, IL-17F, IL-21, IL-23, IL-25, IL-31, Interferon γ (IFNγ), CD40 ligand (CD40L), tumor necrosis factor α (TNFα), CXCL1, CXCL2, CXCL5, CCL3, CCL7 and CCL20.
3Surgery-induced WF promotes the proliferation and migration of breast cancer cells. The composition of WF is influenced by various clinical features and provides potential therapeutic targets to control local recurrence and tumor progression.
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abstractTo clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines.
doi10.18632/oncotarget.4828
pmid26313265
date2015-10-06