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YBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma

Y-box binding protein 1 (YBX1) is involved in the multi-tumor occurrence and development. However, the regulation of YBX1 in lung tumorigenesis and the underlying mechanisms, especially its relationship with CDC25a, was remains unclear. In this study, we analyzed the expression and clinical signific... Full description

Journal Title: Oncotarget 13 December 2016, Vol.7(50), pp.82139-82157
Main Author: Zhao, Shilei
Other Authors: Wang, Yan , Guo, Tao , Yu, Wendan , Li, Jinxiu , Tang, Zhipeng , Yu, Zhenlong , Zhao, Lei , Zhang, Yixiang , Wang, Ziyi , Wang, Peng , Li, Yechi , Li, Fengzhou , Sun, Zhe , Xuan, Yang , Tang, Ranran , Deng, Wu-Guo , Guo, Wei , Gu, Chundong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1949-2553 ; PMID: 27384875 Version:1 ; DOI: 10.18632/oncotarget.10080
Link: http://pubmed.gov/27384875
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recordid: medline27384875
title: YBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma
format: Article
creator:
  • Zhao, Shilei
  • Wang, Yan
  • Guo, Tao
  • Yu, Wendan
  • Li, Jinxiu
  • Tang, Zhipeng
  • Yu, Zhenlong
  • Zhao, Lei
  • Zhang, Yixiang
  • Wang, Ziyi
  • Wang, Peng
  • Li, Yechi
  • Li, Fengzhou
  • Sun, Zhe
  • Xuan, Yang
  • Tang, Ranran
  • Deng, Wu-Guo
  • Guo, Wei
  • Gu, Chundong
subjects:
  • Cdc25a
  • Ybx1
  • Cell Cycle Regulation
  • Lung Adenocarcinoma
  • Prognosis
  • Cell Proliferation
  • Adenocarcinoma -- Enzymology
  • Lung Neoplasms -- Enzymology
  • Y-Box-Binding Protein 1 -- Metabolism
  • Cdc25 Phosphatases -- Metabolism
ispartof: Oncotarget, 13 December 2016, Vol.7(50), pp.82139-82157
description: Y-box binding protein 1 (YBX1) is involved in the multi-tumor occurrence and development. However, the regulation of YBX1 in lung tumorigenesis and the underlying mechanisms, especially its relationship with CDC25a, was remains unclear. In this study, we analyzed the expression and clinical significance of YBX1 and CDC25a in lung adenocarcinoma and identified their roles in the regulation of lung cancer growth. The retrospective analysis of 116 patients with lung adenocarcinoma indicated that YBX1 was positively correlated with CDC25a expression. The Cox-regression analysis showed only high-ranking TNM stage and low CDC25a expression were an independent risk factor of prognosis in enrolled patients. High expression of YBX1 or CDC25a protein was also observed in lung adenocarcinoma cells compared with HLF cells. ChIP assay demonstrated the binding of endogenous YBX1 to the CDC25a promoter region. Overexpression of exogenous YBX1 up-regulated the expression of the CDC25a promoter-driven luciferase. By contrast, inhibition of YBX1 by siRNA markedly decreased the capability of YBX1 binding to CDC25a promoter in A549 and H322 cells. Inhibition of YBX1 expression also blocked cell cycle progression, suppressed cell proliferation and induced apoptosis via the CDC25a pathway in vitro. Moreover, inhibition of YBX1 by siRNA suppressed tumorigenesis in a xenograft mouse model and down-regulated the expression of YBX1, CDC25a, Ki67 and cleaved caspase 3 in the tumor tissues of mice. Collectively, these results demonstrate inhibition of YBX1 suppressed lung cancer growth partly via the CDC25a pathway and high expression of YBX1/CDC25a predicts poor prognosis in human lung adenocarcinoma.
language: eng
source:
identifier: E-ISSN: 1949-2553 ; PMID: 27384875 Version:1 ; DOI: 10.18632/oncotarget.10080
fulltext: fulltext
issn:
  • 19492553
  • 1949-2553
url: Link


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titleYBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma
creatorZhao, Shilei ; Wang, Yan ; Guo, Tao ; Yu, Wendan ; Li, Jinxiu ; Tang, Zhipeng ; Yu, Zhenlong ; Zhao, Lei ; Zhang, Yixiang ; Wang, Ziyi ; Wang, Peng ; Li, Yechi ; Li, Fengzhou ; Sun, Zhe ; Xuan, Yang ; Tang, Ranran ; Deng, Wu-Guo ; Guo, Wei ; Gu, Chundong
ispartofOncotarget, 13 December 2016, Vol.7(50), pp.82139-82157
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subjectCdc25a ; Ybx1 ; Cell Cycle Regulation ; Lung Adenocarcinoma ; Prognosis ; Cell Proliferation ; Adenocarcinoma -- Enzymology ; Lung Neoplasms -- Enzymology ; Y-Box-Binding Protein 1 -- Metabolism ; Cdc25 Phosphatases -- Metabolism
descriptionY-box binding protein 1 (YBX1) is involved in the multi-tumor occurrence and development. However, the regulation of YBX1 in lung tumorigenesis and the underlying mechanisms, especially its relationship with CDC25a, was remains unclear. In this study, we analyzed the expression and clinical significance of YBX1 and CDC25a in lung adenocarcinoma and identified their roles in the regulation of lung cancer growth. The retrospective analysis of 116 patients with lung adenocarcinoma indicated that YBX1 was positively correlated with CDC25a expression. The Cox-regression analysis showed only high-ranking TNM stage and low CDC25a expression were an independent risk factor of prognosis in enrolled patients. High expression of YBX1 or CDC25a protein was also observed in lung adenocarcinoma cells compared with HLF cells. ChIP assay demonstrated the binding of endogenous YBX1 to the CDC25a promoter region. Overexpression of exogenous YBX1 up-regulated the expression of the CDC25a promoter-driven luciferase. By contrast, inhibition of YBX1 by siRNA markedly decreased the capability of YBX1 binding to CDC25a promoter in A549 and H322 cells. Inhibition of YBX1 expression also blocked cell cycle progression, suppressed cell proliferation and induced apoptosis via the CDC25a pathway in vitro. Moreover, inhibition of YBX1 by siRNA suppressed tumorigenesis in a xenograft mouse model and down-regulated the expression of YBX1, CDC25a, Ki67 and cleaved caspase 3 in the tumor tissues of mice. Collectively, these results demonstrate inhibition of YBX1 suppressed lung cancer growth partly via the CDC25a pathway and high expression of YBX1/CDC25a predicts poor prognosis in human lung adenocarcinoma.
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titleYBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma
descriptionY-box binding protein 1 (YBX1) is involved in the multi-tumor occurrence and development. However, the regulation of YBX1 in lung tumorigenesis and the underlying mechanisms, especially its relationship with CDC25a, was remains unclear. In this study, we analyzed the expression and clinical significance of YBX1 and CDC25a in lung adenocarcinoma and identified their roles in the regulation of lung cancer growth. The retrospective analysis of 116 patients with lung adenocarcinoma indicated that YBX1 was positively correlated with CDC25a expression. The Cox-regression analysis showed only high-ranking TNM stage and low CDC25a expression were an independent risk factor of prognosis in enrolled patients. High expression of YBX1 or CDC25a protein was also observed in lung adenocarcinoma cells compared with HLF cells. ChIP assay demonstrated the binding of endogenous YBX1 to the CDC25a promoter region. Overexpression of exogenous YBX1 up-regulated the expression of the CDC25a promoter-driven luciferase. By contrast, inhibition of YBX1 by siRNA markedly decreased the capability of YBX1 binding to CDC25a promoter in A549 and H322 cells. Inhibition of YBX1 expression also blocked cell cycle progression, suppressed cell proliferation and induced apoptosis via the CDC25a pathway in vitro. Moreover, inhibition of YBX1 by siRNA suppressed tumorigenesis in a xenograft mouse model and down-regulated the expression of YBX1, CDC25a, Ki67 and cleaved caspase 3 in the tumor tissues of mice. Collectively, these results demonstrate inhibition of YBX1 suppressed lung cancer growth partly via the CDC25a pathway and high expression of YBX1/CDC25a predicts poor prognosis in human lung adenocarcinoma.
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abstractY-box binding protein 1 (YBX1) is involved in the multi-tumor occurrence and development. However, the regulation of YBX1 in lung tumorigenesis and the underlying mechanisms, especially its relationship with CDC25a, was remains unclear. In this study, we analyzed the expression and clinical significance of YBX1 and CDC25a in lung adenocarcinoma and identified their roles in the regulation of lung cancer growth. The retrospective analysis of 116 patients with lung adenocarcinoma indicated that YBX1 was positively correlated with CDC25a expression. The Cox-regression analysis showed only high-ranking TNM stage and low CDC25a expression were an independent risk factor of prognosis in enrolled patients. High expression of YBX1 or CDC25a protein was also observed in lung adenocarcinoma cells compared with HLF cells. ChIP assay demonstrated the binding of endogenous YBX1 to the CDC25a promoter region. Overexpression of exogenous YBX1 up-regulated the expression of the CDC25a promoter-driven luciferase. By contrast, inhibition of YBX1 by siRNA markedly decreased the capability of YBX1 binding to CDC25a promoter in A549 and H322 cells. Inhibition of YBX1 expression also blocked cell cycle progression, suppressed cell proliferation and induced apoptosis via the CDC25a pathway in vitro. Moreover, inhibition of YBX1 by siRNA suppressed tumorigenesis in a xenograft mouse model and down-regulated the expression of YBX1, CDC25a, Ki67 and cleaved caspase 3 in the tumor tissues of mice. Collectively, these results demonstrate inhibition of YBX1 suppressed lung cancer growth partly via the CDC25a pathway and high expression of YBX1/CDC25a predicts poor prognosis in human lung adenocarcinoma.
doi10.18632/oncotarget.10080
pmid27384875
oafree_for_read
date2016-12-13