schliessen

Filtern

 

Bibliotheken

Multi-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster

Multi-cellular cluster formation of natural killer (NK) cells occurs during in vivo priming and potentiates their activation to IL-2. However, the precise mechanism underlying this synergy within NK cell clusters remains unclear. We employed lymphocyte-laden microwell technologies to modulate contac... Full description

Journal Title: Scientific reports 11 January 2017, Vol.7, pp.40623
Main Author: Kim, Miju
Other Authors: Kim, Tae-Jin , Kim, Hye Mi , Doh, Junsang , Lee, Kyung-Mi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 2045-2322 ; PMID: 28074895 Version:1 ; DOI: 10.1038/srep40623
Link: http://pubmed.gov/28074895
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: medline28074895
title: Multi-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster
format: Article
creator:
  • Kim, Miju
  • Kim, Tae-Jin
  • Kim, Hye Mi
  • Doh, Junsang
  • Lee, Kyung-Mi
subjects:
  • Interleukin-2 -- Metabolism
  • Killer Cells, Natural -- Immunology
  • Lymphocyte Activation -- Immunology
ispartof: Scientific reports, 11 January 2017, Vol.7, pp.40623
description: Multi-cellular cluster formation of natural killer (NK) cells occurs during in vivo priming and potentiates their activation to IL-2. However, the precise mechanism underlying this synergy within NK cell clusters remains unclear. We employed lymphocyte-laden microwell technologies to modulate contact-mediated multi-cellular interactions among activating NK cells and to quantitatively assess the molecular events occurring in multi-cellular clusters of NK cells. NK cells in social microwells, which allow cell-to-cell contact, exhibited significantly higher levels of IL-2 receptor (IL-2R) signaling compared with those in lonesome microwells, which prevent intercellular contact. Further, CD25, an IL-2R α chain, and lytic granules of NK cells in social microwells were polarized toward MTOC. Live cell imaging of lytic granules revealed their dynamic and prolonged polarization toward neighboring NK cells without degranulation. These results suggest that IL-2 bound on CD25 of one NK cells triggered...
language: eng
source:
identifier: E-ISSN: 2045-2322 ; PMID: 28074895 Version:1 ; DOI: 10.1038/srep40623
fulltext: fulltext
issn:
  • 20452322
  • 2045-2322
url: Link


@attributes
ID898186306
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid28074895
sourceidmedline
recordidTN_medline28074895
sourceformatXML
sourcesystemPC
pqid1857752590
display
typearticle
titleMulti-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster
creatorKim, Miju ; Kim, Tae-Jin ; Kim, Hye Mi ; Doh, Junsang ; Lee, Kyung-Mi
ispartofScientific reports, 11 January 2017, Vol.7, pp.40623
identifier
subjectInterleukin-2 -- Metabolism ; Killer Cells, Natural -- Immunology ; Lymphocyte Activation -- Immunology
descriptionMulti-cellular cluster formation of natural killer (NK) cells occurs during in vivo priming and potentiates their activation to IL-2. However, the precise mechanism underlying this synergy within NK cell clusters remains unclear. We employed lymphocyte-laden microwell technologies to modulate contact-mediated multi-cellular interactions among activating NK cells and to quantitatively assess the molecular events occurring in multi-cellular clusters of NK cells. NK cells in social microwells, which allow cell-to-cell contact, exhibited significantly higher levels of IL-2 receptor (IL-2R) signaling compared with those in lonesome microwells, which prevent intercellular contact. Further, CD25, an IL-2R α chain, and lytic granules of NK cells in social microwells were polarized toward MTOC. Live cell imaging of lytic granules revealed their dynamic and prolonged polarization toward neighboring NK cells without degranulation. These results suggest that IL-2 bound on CD25 of one NK cells triggered...
languageeng
source
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://pubmed.gov/28074895$$EView_this_record_in_MEDLINE/PubMed
openurlfulltext$$Topenurlfull_article
addlink$$Uhttp://exlibris-pub.s3.amazonaws.com/aboutMedline.html$$EView_the_MEDLINE/PubMed_Copyright_Statement
search
creatorcontrib
0Kim, Miju
1Kim, Tae-Jin
2Kim, Hye Mi
3Doh, Junsang
4Lee, Kyung-Mi
titleMulti-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster
descriptionMulti-cellular cluster formation of natural killer (NK) cells occurs during in vivo priming and potentiates their activation to IL-2. However, the precise mechanism underlying this synergy within NK cell clusters remains unclear. We employed lymphocyte-laden microwell technologies to modulate contact-mediated multi-cellular interactions among activating NK cells and to quantitatively assess the molecular events occurring in multi-cellular clusters of NK cells. NK cells in social microwells, which allow cell-to-cell contact, exhibited significantly higher levels of IL-2 receptor (IL-2R) signaling compared with those in lonesome microwells, which prevent intercellular contact. Further, CD25, an IL-2R α chain, and lytic granules of NK cells in social microwells were polarized toward MTOC. Live cell imaging of lytic granules revealed their dynamic and prolonged polarization toward neighboring NK cells without degranulation. These results suggest that IL-2 bound on CD25 of one NK cells triggered...
subject
0Interleukin-2 -- Metabolism
1Killer Cells, Natural -- Immunology
2Lymphocyte Activation -- Immunology
general
028074895
1English
2MEDLINE/PubMed (U.S. National Library of Medicine)
310.1038/srep40623
4MEDLINE/PubMed (NLM)
sourceidmedline
recordidmedline28074895
issn
020452322
12045-2322
rsrctypearticle
creationdate2017
addtitleScientific reports
searchscope
0medline
1nlm_medline
2MEDLINE
scope
0medline
1nlm_medline
2MEDLINE
lsr41201711
citationpf 40623 vol 7
startdate20170111
enddate20170111
lsr30VSR-Enriched:[issue, pqid]
sort
titleMulti-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster
authorKim, Miju ; Kim, Tae-Jin ; Kim, Hye Mi ; Doh, Junsang ; Lee, Kyung-Mi
creationdate20170111
lso0120170111
facets
frbrgroupid7207802337646555348
frbrtype5
newrecords20190701
languageeng
creationdate2017
topic
0Interleukin-2–Metabolism
1Killer Cells, Natural–Immunology
2Lymphocyte Activation–Immunology
collectionMEDLINE/PubMed (NLM)
prefilterarticles
rsrctypearticles
creatorcontrib
0Kim, Miju
1Kim, Tae-Jin
2Kim, Hye Mi
3Doh, Junsang
4Lee, Kyung-Mi
jtitleScientific Reports
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Kim
1Doh
2Lee
aufirst
0Miju
1Tae-Jin
2Hye Mi
3Junsang
4Kyung-Mi
au
0Kim, Miju
1Kim, Tae-Jin
2Kim, Hye Mi
3Doh, Junsang
4Lee, Kyung-Mi
atitleMulti-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster
jtitleScientific reports
risdate20170111
volume7
spage40623
pages40623
issn2045-2322
eissn2045-2322
formatjournal
genrearticle
ristypeJOUR
abstractMulti-cellular cluster formation of natural killer (NK) cells occurs during in vivo priming and potentiates their activation to IL-2. However, the precise mechanism underlying this synergy within NK cell clusters remains unclear. We employed lymphocyte-laden microwell technologies to modulate contact-mediated multi-cellular interactions among activating NK cells and to quantitatively assess the molecular events occurring in multi-cellular clusters of NK cells. NK cells in social microwells, which allow cell-to-cell contact, exhibited significantly higher levels of IL-2 receptor (IL-2R) signaling compared with those in lonesome microwells, which prevent intercellular contact. Further, CD25, an IL-2R α chain, and lytic granules of NK cells in social microwells were polarized toward MTOC. Live cell imaging of lytic granules revealed their dynamic and prolonged polarization toward neighboring NK cells without degranulation. These results suggest that IL-2 bound on CD25 of one NK cells triggered...
doi10.1038/srep40623
pmid28074895
issue1
date2017-01-11