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Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery

The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfect... Full description

Journal Title: Journal of nanobiotechnology 14 November 2017, Vol.15(1), pp.82
Main Author: Yu, Qingtong
Other Authors: Chen, Jingjing , Deng, Wenwen , Cao, Xia , Wang, Yan , Zhou, Jie , Xu, Wenqian , Du, Pan , Wang, Qiang , Yu, Jiangnan , Xu, Ximing
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1477-3155 ; PMID: 29137640 Version:1 ; DOI: 10.1186/s12951-017-0317-y
Link: http://pubmed.gov/29137640
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recordid: medline29137640
title: Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery
format: Article
creator:
  • Yu, Qingtong
  • Chen, Jingjing
  • Deng, Wenwen
  • Cao, Xia
  • Wang, Yan
  • Zhou, Jie
  • Xu, Wenqian
  • Du, Pan
  • Wang, Qiang
  • Yu, Jiangnan
  • Xu, Ximing
subjects:
  • Cationized Polysaccharide
  • Gene Co-Delivery, Nanoparticles
  • Nanoparticles
  • Neural Trans-Differentiation
  • Porphyra Yezoensis
  • Gene Transfer Techniques
  • Nanoparticles
  • Fibroblasts -- Drug Effects
  • Neurons -- Drug Effects
  • Polysaccharides -- Pharmacology
  • Porphyra -- Chemistry
ispartof: Journal of nanobiotechnology, 14 November 2017, Vol.15(1), pp.82
description: The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts. Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT) self-assembled into nanoparticles. Results demonstrated that Ed-PYP-pABT nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF and SHH at 14 days after last transfection. Immunofluorescence and western blot assays also showed robust expression of neural markers including Nestin, GFAP, β-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point. Overall, these findings indicated that the P. yezoensis polysaccharide-based non-viral gene co-delivery system is a promising strategy for the generation of neural cells, which might facilitate the developments in the recovery of neural injuries.
language: eng
source:
identifier: E-ISSN: 1477-3155 ; PMID: 29137640 Version:1 ; DOI: 10.1186/s12951-017-0317-y
fulltext: fulltext
issn:
  • 14773155
  • 1477-3155
url: Link


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titleDirect reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery
creatorYu, Qingtong ; Chen, Jingjing ; Deng, Wenwen ; Cao, Xia ; Wang, Yan ; Zhou, Jie ; Xu, Wenqian ; Du, Pan ; Wang, Qiang ; Yu, Jiangnan ; Xu, Ximing
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subjectCationized Polysaccharide ; Gene Co-Delivery, Nanoparticles ; Nanoparticles ; Neural Trans-Differentiation ; Porphyra Yezoensis ; Gene Transfer Techniques ; Nanoparticles ; Fibroblasts -- Drug Effects ; Neurons -- Drug Effects ; Polysaccharides -- Pharmacology ; Porphyra -- Chemistry
descriptionThe cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts. Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT) self-assembled into nanoparticles. Results demonstrated that Ed-PYP-pABT nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF and SHH at 14 days after last transfection. Immunofluorescence and western blot assays also showed robust expression of neural markers including Nestin, GFAP, β-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point. Overall, these findings indicated that the P. yezoensis polysaccharide-based non-viral gene co-delivery system is a promising strategy for the generation of neural cells, which might facilitate the developments in the recovery of neural injuries.
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titleDirect reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery
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0The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts.
1Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT) self-assembled into nanoparticles. Results demonstrated that Ed-PYP-pABT nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF and SHH at 14 days after last transfection. Immunofluorescence and western blot assays also showed robust expression of neural markers including Nestin, GFAP, β-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point.
2Overall, these findings indicated that the P. yezoensis polysaccharide-based non-viral gene co-delivery system is a promising strategy for the generation of neural cells, which might facilitate the developments in the recovery of neural injuries.
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abstractThe cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts.
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