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Characterization of aptamer-mediated gene delivery system for liver cancer therapy

Liver cancer is a fatal disease with limited therapy options. The recombinant adenovirus expressing tumor-suppressor gene of PTEN (Ad5-PTEN) showed effective antitumor activity against liver cancer. However, its disadvantages produced great limitation on its application, especially its nonspecific a... Full description

Journal Title: Oncotarget 23 January 2018, Vol.9(6), pp.6830-6840
Main Author: Liu, Zhongbing
Other Authors: Sun, Xiaoduan , Xiao, Shuangli , Lin, Yan , Li, Chunhong , Hao, Na , Zhou, Meiling , Deng, Ruolan , Ke, Siyun , Zhong, Zhirong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1949-2553 ; PMID: 29467932 Version:1 ; DOI: 10.18632/oncotarget.23564
Link: http://pubmed.gov/29467932
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recordid: medline29467932
title: Characterization of aptamer-mediated gene delivery system for liver cancer therapy
format: Article
creator:
  • Liu, Zhongbing
  • Sun, Xiaoduan
  • Xiao, Shuangli
  • Lin, Yan
  • Li, Chunhong
  • Hao, Na
  • Zhou, Meiling
  • Deng, Ruolan
  • Ke, Siyun
  • Zhong, Zhirong
subjects:
  • Pten
  • Adenovirus
  • Aptamer Epdt3
  • Cell Migration
  • Liver Cancer
ispartof: Oncotarget, 23 January 2018, Vol.9(6), pp.6830-6840
description: Liver cancer is a fatal disease with limited therapy options. The recombinant adenovirus expressing tumor-suppressor gene of PTEN (Ad5-PTEN) showed effective antitumor activity against liver cancer. However, its disadvantages produced great limitation on its application, especially its nonspecific and toxicity to normal cells and tissues. The epithelial cell adhesion molecule (EpCAM) is over-expressed in some liver cancer cells and an RNA aptamer EpDT3 could specially target to EpCAM-positive cells. Based on this founding, we aimed to design a kind of gene delivery system of EpDT3-mediated Ad5-PTEN (EpDT3-PEG-Ad5-PTEN, EPAP) in which polyethylene glycol was used to be a linker to conjugate EpDT3 with Ad5-PTEN. This strategy may overcome the disadvantages of naked Ad5-PTEN and enhance the antitumor effect on liver cancer. The SDS-PAGE electrophoresis, TBE-PAGE electrophoresis and fluorescence detection were conducted to confirm the successful preparation of EPAP. Compared with the naked...
language: eng
source:
identifier: E-ISSN: 1949-2553 ; PMID: 29467932 Version:1 ; DOI: 10.18632/oncotarget.23564
fulltext: fulltext
issn:
  • 19492553
  • 1949-2553
url: Link


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titleCharacterization of aptamer-mediated gene delivery system for liver cancer therapy
creatorLiu, Zhongbing ; Sun, Xiaoduan ; Xiao, Shuangli ; Lin, Yan ; Li, Chunhong ; Hao, Na ; Zhou, Meiling ; Deng, Ruolan ; Ke, Siyun ; Zhong, Zhirong
ispartofOncotarget, 23 January 2018, Vol.9(6), pp.6830-6840
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subjectPten ; Adenovirus ; Aptamer Epdt3 ; Cell Migration ; Liver Cancer
descriptionLiver cancer is a fatal disease with limited therapy options. The recombinant adenovirus expressing tumor-suppressor gene of PTEN (Ad5-PTEN) showed effective antitumor activity against liver cancer. However, its disadvantages produced great limitation on its application, especially its nonspecific and toxicity to normal cells and tissues. The epithelial cell adhesion molecule (EpCAM) is over-expressed in some liver cancer cells and an RNA aptamer EpDT3 could specially target to EpCAM-positive cells. Based on this founding, we aimed to design a kind of gene delivery system of EpDT3-mediated Ad5-PTEN (EpDT3-PEG-Ad5-PTEN, EPAP) in which polyethylene glycol was used to be a linker to conjugate EpDT3 with Ad5-PTEN. This strategy may overcome the disadvantages of naked Ad5-PTEN and enhance the antitumor effect on liver cancer. The SDS-PAGE electrophoresis, TBE-PAGE electrophoresis and fluorescence detection were conducted to confirm the successful preparation of EPAP. Compared with the naked...
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titleCharacterization of aptamer-mediated gene delivery system for liver cancer therapy
descriptionLiver cancer is a fatal disease with limited therapy options. The recombinant adenovirus expressing tumor-suppressor gene of PTEN (Ad5-PTEN) showed effective antitumor activity against liver cancer. However, its disadvantages produced great limitation on its application, especially its nonspecific and toxicity to normal cells and tissues. The epithelial cell adhesion molecule (EpCAM) is over-expressed in some liver cancer cells and an RNA aptamer EpDT3 could specially target to EpCAM-positive cells. Based on this founding, we aimed to design a kind of gene delivery system of EpDT3-mediated Ad5-PTEN (EpDT3-PEG-Ad5-PTEN, EPAP) in which polyethylene glycol was used to be a linker to conjugate EpDT3 with Ad5-PTEN. This strategy may overcome the disadvantages of naked Ad5-PTEN and enhance the antitumor effect on liver cancer. The SDS-PAGE electrophoresis, TBE-PAGE electrophoresis and fluorescence detection were conducted to confirm the successful preparation of EPAP. Compared with the naked...
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abstractLiver cancer is a fatal disease with limited therapy options. The recombinant adenovirus expressing tumor-suppressor gene of PTEN (Ad5-PTEN) showed effective antitumor activity against liver cancer. However, its disadvantages produced great limitation on its application, especially its nonspecific and toxicity to normal cells and tissues. The epithelial cell adhesion molecule (EpCAM) is over-expressed in some liver cancer cells and an RNA aptamer EpDT3 could specially target to EpCAM-positive cells. Based on this founding, we aimed to design a kind of gene delivery system of EpDT3-mediated Ad5-PTEN (EpDT3-PEG-Ad5-PTEN, EPAP) in which polyethylene glycol was used to be a linker to conjugate EpDT3 with Ad5-PTEN. This strategy may overcome the disadvantages of naked Ad5-PTEN and enhance the antitumor effect on liver cancer. The SDS-PAGE electrophoresis, TBE-PAGE electrophoresis and fluorescence detection were conducted to confirm the successful preparation of EPAP. Compared with the naked...
doi10.18632/oncotarget.23564
pmid29467932
date2018-01-23