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Precise Antibody-Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution

Innovative detection techniques to achieve precise m6A distribution within mammalian transcriptome can advance our understanding of its biological functions. We specifically introduced the atom-specific replacement of oxygen with progressively larger atoms (sulfur and selenium) at 4-position of deox... Full description

Journal Title: Journal of the American Chemical Society 09 May 2018, Vol.140(18), pp.5886-5889
Main Author: Hong, Tingting
Other Authors: Yuan, Yushu , Chen, Zonggui , Xi, Kun , Wang, Tianlu , Xie, Yalun , He, Zhiyong , Su, Haomiao , Zhou, Yu , Tan, Zhi-Jie , Weng, Xiaocheng , Zhou, Xiang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-5126 ; PMID: 29489347 Version:1 ; DOI: 10.1021/jacs.7b13633
Link: http://pubmed.gov/29489347
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recordid: medline29489347
title: Precise Antibody-Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution
format: Article
creator:
  • Hong, Tingting
  • Yuan, Yushu
  • Chen, Zonggui
  • Xi, Kun
  • Wang, Tianlu
  • Xie, Yalun
  • He, Zhiyong
  • Su, Haomiao
  • Zhou, Yu
  • Tan, Zhi-Jie
  • Weng, Xiaocheng
  • Zhou, Xiang
subjects:
  • Antibodies -- Chemistry
  • DNA, Single-Stranded -- Chemistry
  • Methyltransferases -- Analysis
  • Selenium -- Chemistry
  • Thymine Nucleotides -- Chemistry
ispartof: Journal of the American Chemical Society, 09 May 2018, Vol.140(18), pp.5886-5889
description: Innovative detection techniques to achieve precise m6A distribution within mammalian transcriptome can advance our understanding of its biological functions. We specifically introduced the atom-specific replacement of oxygen with progressively larger atoms (sulfur and selenium) at 4-position of deoxythymidine triphosphate to weaken its ability to base pair with m6A, while maintaining A-T* base pair virtually the same as the natural one. 4SedTTP turned out to be an outstanding candidate that endowed m6A with a specific signature of RT truncation, thereby making this "RT-silent" modification detectable with the assistance of m6A demethylase FTO through next-generation sequencing. This antibody-independent, 4SedTTP-involved and FTO-assisted strategy is applicable in m6A identification, even for two closely gathered m6A sites, within an unknown region at single-nucleotide resolution.
language: eng
source:
identifier: E-ISSN: 1520-5126 ; PMID: 29489347 Version:1 ; DOI: 10.1021/jacs.7b13633
fulltext: no_fulltext
issn:
  • 15205126
  • 1520-5126
url: Link


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titlePrecise Antibody-Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution
creatorHong, Tingting ; Yuan, Yushu ; Chen, Zonggui ; Xi, Kun ; Wang, Tianlu ; Xie, Yalun ; He, Zhiyong ; Su, Haomiao ; Zhou, Yu ; Tan, Zhi-Jie ; Weng, Xiaocheng ; Zhou, Xiang
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subjectAntibodies -- Chemistry ; DNA, Single-Stranded -- Chemistry ; Methyltransferases -- Analysis ; Selenium -- Chemistry ; Thymine Nucleotides -- Chemistry
descriptionInnovative detection techniques to achieve precise m6A distribution within mammalian transcriptome can advance our understanding of its biological functions. We specifically introduced the atom-specific replacement of oxygen with progressively larger atoms (sulfur and selenium) at 4-position of deoxythymidine triphosphate to weaken its ability to base pair with m6A, while maintaining A-T* base pair virtually the same as the natural one. 4SedTTP turned out to be an outstanding candidate that endowed m6A with a specific signature of RT truncation, thereby making this "RT-silent" modification detectable with the assistance of m6A demethylase FTO through next-generation sequencing. This antibody-independent, 4SedTTP-involved and FTO-assisted strategy is applicable in m6A identification, even for two closely gathered m6A sites, within an unknown region at single-nucleotide resolution.
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titlePrecise Antibody-Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution
descriptionInnovative detection techniques to achieve precise m6A distribution within mammalian transcriptome can advance our understanding of its biological functions. We specifically introduced the atom-specific replacement of oxygen with progressively larger atoms (sulfur and selenium) at 4-position of deoxythymidine triphosphate to weaken its ability to base pair with m6A, while maintaining A-T* base pair virtually the same as the natural one. 4SedTTP turned out to be an outstanding candidate that endowed m6A with a specific signature of RT truncation, thereby making this "RT-silent" modification detectable with the assistance of m6A demethylase FTO through next-generation sequencing. This antibody-independent, 4SedTTP-involved and FTO-assisted strategy is applicable in m6A identification, even for two closely gathered m6A sites, within an unknown region at single-nucleotide resolution.
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titlePrecise Antibody-Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution
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abstractInnovative detection techniques to achieve precise m6A distribution within mammalian transcriptome can advance our understanding of its biological functions. We specifically introduced the atom-specific replacement of oxygen with progressively larger atoms (sulfur and selenium) at 4-position of deoxythymidine triphosphate to weaken its ability to base pair with m6A, while maintaining A-T* base pair virtually the same as the natural one. 4SedTTP turned out to be an outstanding candidate that endowed m6A with a specific signature of RT truncation, thereby making this "RT-silent" modification detectable with the assistance of m6A demethylase FTO through next-generation sequencing. This antibody-independent, 4SedTTP-involved and FTO-assisted strategy is applicable in m6A identification, even for two closely gathered m6A sites, within an unknown region at single-nucleotide resolution.
doi10.1021/jacs.7b13633
pmid29489347
issn00027863
oafree_for_read
date2018-05-09