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Activatable Near-Infrared Fluorescent Probe for Dipeptidyl Peptidase IV and Its Bioimaging Applications in Living Cells and Animals

Visualization of endogenous disease-associated enzymes is of great clinical significance, as it could allow earlier clinical diagnosis and timely intervention. Herein, we first synthesized and characterized an enzyme-activatable near-infrared fluorescent probe, GP-DM, for determining the activity of... Full description

Journal Title: Analytical chemistry 20 March 2018, Vol.90(6), pp.3965-3973
Main Author: Liu, Tao
Other Authors: Ning, Jing , Wang, Bo , Dong, Bin , Li, Song , Tian, Xiangge , Yu, Zhenlong , Peng, Yulin , Wang, Chao , Zhao, Xinyu , Huo, Xiaokui , Sun, Chengpeng , Cui, Jingnan , Feng, Lei , Ma, Xiaochi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; PMID: 29493228 Version:1 ; DOI: 10.1021/acs.analchem.7b04957
Link: http://pubmed.gov/29493228
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title: Activatable Near-Infrared Fluorescent Probe for Dipeptidyl Peptidase IV and Its Bioimaging Applications in Living Cells and Animals
format: Article
creator:
  • Liu, Tao
  • Ning, Jing
  • Wang, Bo
  • Dong, Bin
  • Li, Song
  • Tian, Xiangge
  • Yu, Zhenlong
  • Peng, Yulin
  • Wang, Chao
  • Zhao, Xinyu
  • Huo, Xiaokui
  • Sun, Chengpeng
  • Cui, Jingnan
  • Feng, Lei
  • Ma, Xiaochi
subjects:
  • Cells
  • Animals
  • Visualization
  • Enzymes
  • Peptides
  • Tumors
  • Catalytic Activity
  • Cancer
  • Image Detection
  • Medical Imaging
  • Catalysis
  • Zebrafish
  • Migration
  • Biological Properties
  • Biological Activity
  • Therapy
  • Fluorescent Indicators
  • Diabetes Mellitus
ispartof: Analytical chemistry, 20 March 2018, Vol.90(6), pp.3965-3973
description: Visualization of endogenous disease-associated enzymes is of great clinical significance, as it could allow earlier clinical diagnosis and timely intervention. Herein, we first synthesized and characterized an enzyme-activatable near-infrared fluorescent probe, GP-DM, for determining the activity of dipeptidyl peptidase IV (DPP IV), which is associated with various pathological processes, especially in diabetes and malignant tumors. GP-DM emitted significant turn-on NIR fluorescent signals simultaneously in response to DPP IV, making it favorable for accurately and dynamically monitoring DPP IV activity in vitro and in vivo. GP-DM exhibited excellent specificity and sensitivity in DPP IV imaging, as indicated by its higher catalytic activity than other human serine hydrolases and by its strong anti-interference ability to a complex biological matrix, which was fully characterized in a series of phenotyping reactions and inhibition assays. Encouraged by the advantages mentioned above, we successfully used GP-DM to evaluate endogenous DPP IV activity in various biological samples (plasma and tissue preparations) and living tumor cells and performed real-time in vivo bioimaging of DPP IV in zebrafish and tumor-bearing nude mice. All of the results reflected and highlighted the potential application value of GP-DM in the early detection of pathologies, individual tailoring of drug therapy, and image-guided tumor resection. Furthermore, our results revealed that DPP IV, a key target enzyme, is closely associated with the migration and proliferation of cancer cells and regulating the biological activity of DPP IV may be a useful approach for cancer therapy.
language: eng
source:
identifier: E-ISSN: 1520-6882 ; PMID: 29493228 Version:1 ; DOI: 10.1021/acs.analchem.7b04957
fulltext: no_fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleActivatable Near-Infrared Fluorescent Probe for Dipeptidyl Peptidase IV and Its Bioimaging Applications in Living Cells and Animals
creatorLiu, Tao ; Ning, Jing ; Wang, Bo ; Dong, Bin ; Li, Song ; Tian, Xiangge ; Yu, Zhenlong ; Peng, Yulin ; Wang, Chao ; Zhao, Xinyu ; Huo, Xiaokui ; Sun, Chengpeng ; Cui, Jingnan ; Feng, Lei ; Ma, Xiaochi
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descriptionVisualization of endogenous disease-associated enzymes is of great clinical significance, as it could allow earlier clinical diagnosis and timely intervention. Herein, we first synthesized and characterized an enzyme-activatable near-infrared fluorescent probe, GP-DM, for determining the activity of dipeptidyl peptidase IV (DPP IV), which is associated with various pathological processes, especially in diabetes and malignant tumors. GP-DM emitted significant turn-on NIR fluorescent signals simultaneously in response to DPP IV, making it favorable for accurately and dynamically monitoring DPP IV activity in vitro and in vivo. GP-DM exhibited excellent specificity and sensitivity in DPP IV imaging, as indicated by its higher catalytic activity than other human serine hydrolases and by its strong anti-interference ability to a complex biological matrix, which was fully characterized in a series of phenotyping reactions and inhibition assays. Encouraged by the advantages mentioned above, we successfully used GP-DM to evaluate endogenous DPP IV activity in various biological samples (plasma and tissue preparations) and living tumor cells and performed real-time in vivo bioimaging of DPP IV in zebrafish and tumor-bearing nude mice. All of the results reflected and highlighted the potential application value of GP-DM in the early detection of pathologies, individual tailoring of drug therapy, and image-guided tumor resection. Furthermore, our results revealed that DPP IV, a key target enzyme, is closely associated with the migration and proliferation of cancer cells and regulating the biological activity of DPP IV may be a useful approach for cancer therapy.
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abstractVisualization of endogenous disease-associated enzymes is of great clinical significance, as it could allow earlier clinical diagnosis and timely intervention. Herein, we first synthesized and characterized an enzyme-activatable near-infrared fluorescent probe, GP-DM, for determining the activity of dipeptidyl peptidase IV (DPP IV), which is associated with various pathological processes, especially in diabetes and malignant tumors. GP-DM emitted significant turn-on NIR fluorescent signals simultaneously in response to DPP IV, making it favorable for accurately and dynamically monitoring DPP IV activity in vitro and in vivo. GP-DM exhibited excellent specificity and sensitivity in DPP IV imaging, as indicated by its higher catalytic activity than other human serine hydrolases and by its strong anti-interference ability to a complex biological matrix, which was fully characterized in a series of phenotyping reactions and inhibition assays. Encouraged by the advantages mentioned above, we successfully used GP-DM to evaluate endogenous DPP IV activity in various biological samples (plasma and tissue preparations) and living tumor cells and performed real-time in vivo bioimaging of DPP IV in zebrafish and tumor-bearing nude mice. All of the results reflected and highlighted the potential application value of GP-DM in the early detection of pathologies, individual tailoring of drug therapy, and image-guided tumor resection. Furthermore, our results revealed that DPP IV, a key target enzyme, is closely associated with the migration and proliferation of cancer cells and regulating the biological activity of DPP IV may be a useful approach for cancer therapy.
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