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Heat stress impairs gap junction communication and cumulus function of bovine oocytes

The intimate association of cumulus cells with one another and with the oocyte is important for regulating oocyte meiotic arrest and resumption. The objective of this study was to determine the effects of heat stress on cumulus cell communication and functions that may be related to accelerated oocy... Full description

Journal Title: The Journal of reproduction and development 12 October 2018, Vol.64(5), pp.385-392
Main Author: Campen, Kelly A
Other Authors: Abbott, Chelsea R , Rispoli, Louisa A , Payton, Rebecca R , Saxton, Arnold M , Edwards, J Lannett
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1348-4400 ; PMID: 29937465 Version:1 ; DOI: 10.1262/jrd.2018-029
Link: http://pubmed.gov/29937465
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recordid: medline29937465
title: Heat stress impairs gap junction communication and cumulus function of bovine oocytes
format: Article
creator:
  • Campen, Kelly A
  • Abbott, Chelsea R
  • Rispoli, Louisa A
  • Payton, Rebecca R
  • Saxton, Arnold M
  • Edwards, J Lannett
subjects:
  • Cumulus Cell
  • Gap Junction
  • Heat Stress
  • Meiotic Maturation
  • Oocyte
  • Heat-Shock Response
  • Hot Temperature
  • Cumulus Cells -- Metabolism
  • Gap Junctions -- Metabolism
  • Oocytes -- Cytology
ispartof: The Journal of reproduction and development, 12 October 2018, Vol.64(5), pp.385-392
description: The intimate association of cumulus cells with one another and with the oocyte is important for regulating oocyte meiotic arrest and resumption. The objective of this study was to determine the effects of heat stress on cumulus cell communication and functions that may be related to accelerated oocyte meiosis during early maturation. Bovine cumulus-oocyte complexes underwent in vitro maturation for up to 6 h at thermoneutral control (38.5°C) or elevated (40.0, 41.0 or 42.0°C) temperatures. Gap junction communication between the cumulus cells and the oocyte was assessed using the fluorescent dye calcein after 4 h of in vitro maturation. Dye transfer was reduced in cumulus-oocyte complexes matured at 41.0°C or 42.0°C; transfer at 40.0°C was similar to control (P < 0.0001). Subsequent staining of oocytes with Hoechst revealed that oocytes matured at 41.0 or 42.0°C contained chromatin at more advanced stages of condensation. Maturation of cumulus-oocyte complexes at elevated temperatures reduced...
language: eng
source:
identifier: E-ISSN: 1348-4400 ; PMID: 29937465 Version:1 ; DOI: 10.1262/jrd.2018-029
fulltext: fulltext
issn:
  • 13484400
  • 1348-4400
url: Link


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titleHeat stress impairs gap junction communication and cumulus function of bovine oocytes
creatorCampen, Kelly A ; Abbott, Chelsea R ; Rispoli, Louisa A ; Payton, Rebecca R ; Saxton, Arnold M ; Edwards, J Lannett
ispartofThe Journal of reproduction and development, 12 October 2018, Vol.64(5), pp.385-392
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subjectCumulus Cell ; Gap Junction ; Heat Stress ; Meiotic Maturation ; Oocyte ; Heat-Shock Response ; Hot Temperature ; Cumulus Cells -- Metabolism ; Gap Junctions -- Metabolism ; Oocytes -- Cytology
descriptionThe intimate association of cumulus cells with one another and with the oocyte is important for regulating oocyte meiotic arrest and resumption. The objective of this study was to determine the effects of heat stress on cumulus cell communication and functions that may be related to accelerated oocyte meiosis during early maturation. Bovine cumulus-oocyte complexes underwent in vitro maturation for up to 6 h at thermoneutral control (38.5°C) or elevated (40.0, 41.0 or 42.0°C) temperatures. Gap junction communication between the cumulus cells and the oocyte was assessed using the fluorescent dye calcein after 4 h of in vitro maturation. Dye transfer was reduced in cumulus-oocyte complexes matured at 41.0°C or 42.0°C; transfer at 40.0°C was similar to control (P < 0.0001). Subsequent staining of oocytes with Hoechst revealed that oocytes matured at 41.0 or 42.0°C contained chromatin at more advanced stages of condensation. Maturation of cumulus-oocyte complexes at elevated temperatures reduced...
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titleHeat stress impairs gap junction communication and cumulus function of bovine oocytes
descriptionThe intimate association of cumulus cells with one another and with the oocyte is important for regulating oocyte meiotic arrest and resumption. The objective of this study was to determine the effects of heat stress on cumulus cell communication and functions that may be related to accelerated oocyte meiosis during early maturation. Bovine cumulus-oocyte complexes underwent in vitro maturation for up to 6 h at thermoneutral control (38.5°C) or elevated (40.0, 41.0 or 42.0°C) temperatures. Gap junction communication between the cumulus cells and the oocyte was assessed using the fluorescent dye calcein after 4 h of in vitro maturation. Dye transfer was reduced in cumulus-oocyte complexes matured at 41.0°C or 42.0°C; transfer at 40.0°C was similar to control (P < 0.0001). Subsequent staining of oocytes with Hoechst revealed that oocytes matured at 41.0 or 42.0°C contained chromatin at more advanced stages of condensation. Maturation of cumulus-oocyte complexes at elevated temperatures reduced...
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abstractThe intimate association of cumulus cells with one another and with the oocyte is important for regulating oocyte meiotic arrest and resumption. The objective of this study was to determine the effects of heat stress on cumulus cell communication and functions that may be related to accelerated oocyte meiosis during early maturation. Bovine cumulus-oocyte complexes underwent in vitro maturation for up to 6 h at thermoneutral control (38.5°C) or elevated (40.0, 41.0 or 42.0°C) temperatures. Gap junction communication between the cumulus cells and the oocyte was assessed using the fluorescent dye calcein after 4 h of in vitro maturation. Dye transfer was reduced in cumulus-oocyte complexes matured at 41.0°C or 42.0°C; transfer at 40.0°C was similar to control (P < 0.0001). Subsequent staining of oocytes with Hoechst revealed that oocytes matured at 41.0 or 42.0°C contained chromatin at more advanced stages of condensation. Maturation of cumulus-oocyte complexes at elevated temperatures reduced...
doi10.1262/jrd.2018-029
pmid29937465
date2018-10-12