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Generation of lymphohematopoietic cells from embryonic stem cells in culture

An efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages by coculture with the stromal cell line OP9. This cell line does not express functional macrophage colony-stimulating factor (M-CSF). The presen... Full description

Journal Title: Science (New York N.Y.), 19 August 1994, Vol.265(5175), pp.1098-101
Main Author: Nakano, T
Other Authors: Kodama, H , Honjo, T
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0036-8075 ; PMID: 8066449 Version:1
Link: http://pubmed.gov/8066449
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recordid: medline8066449
title: Generation of lymphohematopoietic cells from embryonic stem cells in culture
format: Article
creator:
  • Nakano, T
  • Kodama, H
  • Honjo, T
subjects:
  • Hematopoiesis
  • Hematopoietic Stem Cells -- Cytology
  • Lymphocytes -- Cytology
ispartof: Science (New York, N.Y.), 19 August 1994, Vol.265(5175), pp.1098-101
description: An efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages by coculture with the stromal cell line OP9. This cell line does not express functional macrophage colony-stimulating factor (M-CSF). The presence of M-CSF had inhibitory effects on the differentiation of ES cells to blood cells other than macrophages. Embryoid body formation or addition of exogenous growth factors was not required, and differentiation was highly reproducible even after the selection of ES cells with the antibiotic G418. Combined with the ability to genetically manipulate ES cells, this system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells.
language: eng
source:
identifier: ISSN: 0036-8075 ; PMID: 8066449 Version:1
fulltext: fulltext
issn:
  • 00368075
  • 0036-8075
url: Link


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titleGeneration of lymphohematopoietic cells from embryonic stem cells in culture
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identifierISSN: 0036-8075 ; PMID: 8066449 Version:1
subjectHematopoiesis ; Hematopoietic Stem Cells -- Cytology ; Lymphocytes -- Cytology
descriptionAn efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages by coculture with the stromal cell line OP9. This cell line does not express functional macrophage colony-stimulating factor (M-CSF). The presence of M-CSF had inhibitory effects on the differentiation of ES cells to blood cells other than macrophages. Embryoid body formation or addition of exogenous growth factors was not required, and differentiation was highly reproducible even after the selection of ES cells with the antibiotic G418. Combined with the ability to genetically manipulate ES cells, this system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells.
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abstractAn efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages by coculture with the stromal cell line OP9. This cell line does not express functional macrophage colony-stimulating factor (M-CSF). The presence of M-CSF had inhibitory effects on the differentiation of ES cells to blood cells other than macrophages. Embryoid body formation or addition of exogenous growth factors was not required, and differentiation was highly reproducible even after the selection of ES cells with the antibiotic G418. Combined with the ability to genetically manipulate ES cells, this system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells.
pmid8066449
doi10.1126/science.8066449
eissn10959203
date1994-08-19