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Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis

Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progress... Full description

Journal Title: International Journal of Oral Science 2013, Vol.6(1), p.31
Main Author: Wei-Peng Jiang
Other Authors: Zi-Han Sima , Hai-Cheng Wang , Jian-Yun Zhang , Li-Sha Sun , Feng Chen , Tie-Jun Li
Format: Electronic Article Electronic Article
Language:
Subjects:
RNA
ID: ISSN: 1674-2818 ; E-ISSN: 2049-3169 ; DOI: 10.1038/ijos.2013.96
Link: http://dx.doi.org/10.1038/ijos.2013.96
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recordid: nature_a10.1038/ijos.2013.96
title: Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis
format: Article
creator:
  • Wei-Peng Jiang
  • Zi-Han Sima
  • Hai-Cheng Wang
  • Jian-Yun Zhang
  • Li-Sha Sun
  • Feng Chen
  • Tie-Jun Li
subjects:
  • Stroma
  • Enzyme-Linked Immunosorbent Assay
  • Data Processing
  • Mucosa
  • Gingiva
  • Angiogenesis
  • Aggressive Behavior
  • Tumors
  • Umbilical Vein
  • Cysts
  • Odontogenic Tumors
  • Mrna
  • Fibroblasts
  • Leukocyte Migration
  • Endothelial Cells
  • Polymerase Chain Reaction
  • Cell Proliferation
  • Immunohistochemistry
  • Benign
  • RNA
  • Angiogenesis
  • Keratocystic Odontogenic Tumor
  • Lysyl Oxidase-Like 4
  • RNA-Sequencing
  • Tumor Stromal Fibroblast
ispartof: International Journal of Oral Science, 2013, Vol.6(1), p.31
description: Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progression, RNA sequencing (RNA-Seq) was performed using samples from KCOT and primary stromal fibroblasts isolated from gingival tissues. Seven candidate genes that possess a function potentially related to KCOT progression were selected and their expression levels were confirmed by quantitative PCR, immunohistochemistry and enzyme-linked immunosorbent assay. Expression of lysyl oxidase-like 4 (LOXL4), the only candidate gene that encodes a secreted protein, was enhanced at both the mRNA and protein levels in KCOT stromal tissues and primary KCOT stromal fibroblasts compared to control tissues and primary fibroblasts (P
language:
source:
identifier: ISSN: 1674-2818 ; E-ISSN: 2049-3169 ; DOI: 10.1038/ijos.2013.96
fulltext: fulltext
issn:
  • 1674-2818
  • 16742818
  • 2049-3169
  • 20493169
url: Link


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titleIdentification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis
creatorWei-Peng Jiang ; Zi-Han Sima ; Hai-Cheng Wang ; Jian-Yun Zhang ; Li-Sha Sun ; Feng Chen ; Tie-Jun Li
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subjectStroma ; Enzyme-Linked Immunosorbent Assay ; Data Processing ; Mucosa ; Gingiva ; Angiogenesis ; Aggressive Behavior ; Tumors ; Umbilical Vein ; Cysts ; Odontogenic Tumors ; Mrna ; Fibroblasts ; Leukocyte Migration ; Endothelial Cells ; Polymerase Chain Reaction ; Cell Proliferation ; Immunohistochemistry ; Benign ; RNA ; Angiogenesis ; Keratocystic Odontogenic Tumor ; Lysyl Oxidase-Like 4 ; RNA-Sequencing ; Tumor Stromal Fibroblast;
descriptionKeratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progression, RNA sequencing (RNA-Seq) was performed using samples from KCOT and primary stromal fibroblasts isolated from gingival tissues. Seven candidate genes that possess a function potentially related to KCOT progression were selected and their expression levels were confirmed by quantitative PCR, immunohistochemistry and enzyme-linked immunosorbent assay. Expression of lysyl oxidase-like 4 (LOXL4), the only candidate gene that encodes a secreted protein, was enhanced at both the mRNA and protein levels in KCOT stromal tissues and primary KCOT stromal fibroblasts compared to control tissues and primary fibroblasts (P<0.05). In vitro, high expression of LOXL4 could enhance proliferation and migration of the human umbilical vein endothelial cells (HUVECs). There was a significant, positive correlation between LOXL4 protein expression and MVD in stroma of KCOT and control tissues (r=0.882). These data suggest that abnormal expression of LOXL4 of KCOT may enhance angiogenesis in KCOT, which may help to promote the locally aggressive biological behavior of KCOT.
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