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Fluorescent indicators for simultaneous reporting of all four cell cycle phases

A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We co... Full description

Journal Title: Nature Methods 2016
Main Author: Bryce T Bajar
Other Authors: Amy J Lam , Ryan K Badiee , Young-Hee Oh , Jun Chu , Xin X Zhou , Namdoo Kim , Benjamin B Kim , Mingyu Chung , Arielle L Yablonovitch , Barney F Cruz , Kanokwan Kulalert , Jacqueline J Tao , Tobias Meyer , Xiao-Dong Su , Michael Z Lin
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ID: ISSN: 1548-7091 ; E-ISSN: 1548-7105 ; DOI: 10.1038/nmeth.4045
Link: http://dx.doi.org/10.1038/nmeth.4045
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recordid: nature_a10.1038/nmeth.4045
title: Fluorescent indicators for simultaneous reporting of all four cell cycle phases
format: Article
creator:
  • Bryce T Bajar
  • Amy J Lam
  • Ryan K Badiee
  • Young-Hee Oh
  • Jun Chu
  • Xin X Zhou
  • Namdoo Kim
  • Benjamin B Kim
  • Mingyu Chung
  • Arielle L Yablonovitch
  • Barney F Cruz
  • Kanokwan Kulalert
  • Jacqueline J Tao
  • Tobias Meyer
  • Xiao-Dong Su
  • Michael Z Lin
subjects:
  • Biology
ispartof: Nature Methods, 2016
description: A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
language:
source:
identifier: ISSN: 1548-7091 ; E-ISSN: 1548-7105 ; DOI: 10.1038/nmeth.4045
fulltext: fulltext
issn:
  • 1548-7091
  • 15487091
  • 1548-7105
  • 15487105
url: Link


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titleFluorescent indicators for simultaneous reporting of all four cell cycle phases
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descriptionA robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
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