schliessen

Filtern

 

Bibliotheken

Small nucleolar RNA 42 acts as an oncogene in lung tumorigenesis

Non-small cell lung cancer (NSCLC) is the leading cause of cancer death, reflecting the need for better understanding the oncogenesis, and developing new diagnostic and therapeutic targets for the malignancy. Emerging evidence suggests that small nucleolar RNAs (snoRNAs) have malfunctioning roles in... Full description

Journal Title: Oncogene 2011, Vol.31(22), p.2794
Main Author: Y-P Mei
Other Authors: J-P Liao , J Shen , L Yu , B-L Liu , L Liu , R-Y Li , L Ji , S G Dorsey , Z-R Jiang , R L Katz , J-Y Wang , F Jiang
Format: Electronic Article Electronic Article
Language:
Subjects:
ID: ISSN: 0950-9232 ; E-ISSN: 1476-5594 ; DOI: 10.1038/onc.2011.449
Link: http://dx.doi.org/10.1038/onc.2011.449
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: nature_a10.1038/onc.2011.449
title: Small nucleolar RNA 42 acts as an oncogene in lung tumorigenesis
format: Article
creator:
  • Y-P Mei
  • J-P Liao
  • J Shen
  • L Yu
  • B-L Liu
  • L Liu
  • R-Y Li
  • L Ji
  • S G Dorsey
  • Z-R Jiang
  • R L Katz
  • J-Y Wang
  • F Jiang
subjects:
  • Non-Small Cell Lung Cancer -- Risk Factors
  • Non-Small Cell Lung Cancer -- Genetic Aspects
  • Non-Small Cell Lung Cancer -- Research
  • Oncogenes -- Physiological Aspects
  • Oncogenes -- Research
  • Rna -- Physiological Aspects
  • Rna -- Research
ispartof: Oncogene, 2011, Vol.31(22), p.2794
description: Non-small cell lung cancer (NSCLC) is the leading cause of cancer death, reflecting the need for better understanding the oncogenesis, and developing new diagnostic and therapeutic targets for the malignancy. Emerging evidence suggests that small nucleolar RNAs (snoRNAs) have malfunctioning roles in tumorigenesis. Our recent study demonstrated that small nucleolar RNA 42 (SNORA42) was overexpressed in lung tumors. Here, we investigate the role of SNORA42 in tumorigenesis of NSCLC. We simultaneously assess genomic dosages and expression levels of SNORA42 and its host gene, KIAA0907, in 10 NSCLC cell lines and a human bronchial epithelial cell line. We then determine in vitro functional significance of SNORA42 in lung cancer cell lines through gain- and loss-of-function analyses. We also inoculate cancer cells with SNORA42-siRNA into mice through either tail vein or subcutaneous injection. We finally evaluate expression level of SNORA42 on frozen surgically resected lung tumor tissues of 64 patients with stage I NSCLC by using quantitative reverse transcriptase PCR assay. Genomic amplification and associated high expression of SNORA42 rather than KIAA0907 are frequently observed in lung cancer cells, suggesting that SNORA42 overexpression is activated by its genomic amplification. SNORA42 knockdown in NSCLC cells inhibits in vitro and in vivo tumorigenicity, whereas enforced SNORA42 expression in bronchial epitheliums increases cell growth and colony formation. Such pleiotropy of SNORA42 suppression could be achieved at least partially through increased apoptosis of NSCLC cells in a p53-dependent manner. SNORA42 expression in lung tumor tissue specimens is inversely correlated with survival of NSCLC patients. Therefore, SNORA42 activation could have an oncogenic role in lung tumorigenesis and provide potential diagnostic and therapeutic targets for the malignancy. Oncogene (2012) 31, 2794-2804; doi: 10.1038/onc.2011.449; published online 10 October 2011 Keywords: small nucleolar RNA; tumorigenesis; biomarkers; apoptosis; p53
language:
source:
identifier: ISSN: 0950-9232 ; E-ISSN: 1476-5594 ; DOI: 10.1038/onc.2011.449
fulltext: fulltext
issn:
  • 0950-9232
  • 09509232
  • 1476-5594
  • 14765594
url: Link


@attributes
ID403814973
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1038/onc.2011.449
sourceidnature_a
recordidTN_nature_a10.1038/onc.2011.449
sourcesystemPC
pqid1020857045
galeid293353448
display
typearticle
titleSmall nucleolar RNA 42 acts as an oncogene in lung tumorigenesis
creatorY-P Mei ; J-P Liao ; J Shen ; L Yu ; B-L Liu ; L Liu ; R-Y Li ; L Ji ; S G Dorsey ; Z-R Jiang ; R L Katz ; J-Y Wang ; F Jiang
ispartofOncogene, 2011, Vol.31(22), p.2794
identifier
source
subjectNon-Small Cell Lung Cancer -- Risk Factors ; Non-Small Cell Lung Cancer -- Genetic Aspects ; Non-Small Cell Lung Cancer -- Research ; Oncogenes -- Physiological Aspects ; Oncogenes -- Research ; Rna -- Physiological Aspects ; Rna -- Research;
descriptionNon-small cell lung cancer (NSCLC) is the leading cause of cancer death, reflecting the need for better understanding the oncogenesis, and developing new diagnostic and therapeutic targets for the malignancy. Emerging evidence suggests that small nucleolar RNAs (snoRNAs) have malfunctioning roles in tumorigenesis. Our recent study demonstrated that small nucleolar RNA 42 (SNORA42) was overexpressed in lung tumors. Here, we investigate the role of SNORA42 in tumorigenesis of NSCLC. We simultaneously assess genomic dosages and expression levels of SNORA42 and its host gene, KIAA0907, in 10 NSCLC cell lines and a human bronchial epithelial cell line. We then determine in vitro functional significance of SNORA42 in lung cancer cell lines through gain- and loss-of-function analyses. We also inoculate cancer cells with SNORA42-siRNA into mice through either tail vein or subcutaneous injection. We finally evaluate expression level of SNORA42 on frozen surgically resected lung tumor tissues of 64 patients with stage I NSCLC by using quantitative reverse transcriptase PCR assay. Genomic amplification and associated high expression of SNORA42 rather than KIAA0907 are frequently observed in lung cancer cells, suggesting that SNORA42 overexpression is activated by its genomic amplification. SNORA42 knockdown in NSCLC cells inhibits in vitro and in vivo tumorigenicity, whereas enforced SNORA42 expression in bronchial epitheliums increases cell growth and colony formation. Such pleiotropy of SNORA42 suppression could be achieved at least partially through increased apoptosis of NSCLC cells in a p53-dependent manner. SNORA42 expression in lung tumor tissue specimens is inversely correlated with survival of NSCLC patients. Therefore, SNORA42 activation could have an oncogenic role in lung tumorigenesis and provide potential diagnostic and therapeutic targets for the malignancy. Oncogene (2012) 31, 2794-2804; doi: 10.1038/onc.2011.449; published online 10 October 2011 Keywords: small nucleolar RNA; tumorigenesis; biomarkers; apoptosis; p53
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://dx.doi.org/10.1038/onc.2011.449$$EView_this_record_in_Nature
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Y-P Mei
1J-P Liao
2J Shen
3L Yu
4B-L Liu
5L Liu
6R-Y Li
7L Ji
8S G Dorsey
9Z-R Jiang
10R L Katz
11J-Y Wang
12F Jiang
titleSmall nucleolar RNA 42 acts as an oncogene in lung tumorigenesis
general
0Nature Publishing Group
110.1038/onc.2011.449
2nature.com (Nature Publishing Group)
sourceidnature_a
recordidnature_a10.1038/onc.2011.449
issn
00950-9232
109509232
21476-5594
314765594
rsrctypearticle
creationdate2011
searchscopenature_a
scopenature_a
lsr30VSR-Enriched:[subject, galeid, description, pages, eissn, pqid]
sort
titleSmall nucleolar RNA 42 acts as an oncogene in lung tumorigenesis
authorY-P Mei ; J-P Liao ; J Shen ; L Yu ; B-L Liu ; L Liu ; R-Y Li ; L Ji ; S G Dorsey ; Z-R Jiang ; R L Katz ; J-Y Wang ; F Jiang
creationdate20111010
facets
frbrgroupid8714401654394522240
frbrtype5
creationdate2011
collectionnature.com (Nature Publishing Group)
prefilterarticles
rsrctypearticles
creatorcontrib
0Y-P Mei
1J-P Liao
2J Shen
3L Yu
4B-L Liu
5L Liu
6R-Y Li
7L Ji
8S G Dorsey
9Z-R Jiang
10R L Katz
11J-Y Wang
12F Jiang
jtitleOncogene
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Y-P Mei
1J-P Liao
2J Shen
3L Yu
4B-L Liu
5L Liu
6R-Y Li
7L Ji
8S G Dorsey
9Z-R Jiang
10R L Katz
11J-Y Wang
12F Jiang
atitleSmall nucleolar RNA 42 acts as an oncogene in lung tumorigenesis
jtitleOncogene
risdate20111010
volume31
issue22
spage2794
issn0950-9232
genrearticle
ristypeJOUR
pubNature Publishing Group
doi10.1038/onc.2011.449
pages2794-2794804
eissn14765594
date2011-10-10