schliessen

Filtern

 

Bibliotheken

Tenascin-C Is Expressed in Macrophage-Rich Human Coronary Atherosclerotic Plaque

BACKGROUND: Tenascin is a large extracellular matrix glycoprotein generally found in adult tissues undergoing active remodeling such as healing wounds and tumors. To determine the potential role of tenascin-C (TN-C) in the pathophysiology of atherosclerosis, we investigated the pattern of expression... Full description

Journal Title: Circulation 1999, Vol.99(10), pp.1284-1289
Main Author: Wallner, Kurt
Other Authors: Li, Chen , Shah, Prediman K. , Fishbein, Michael C. , Forrester, James S. , Kaul, Sanjay , Sharifi, Behrooz G.
Format: Electronic Article Electronic Article
Language:
Subjects:
ID: ISSN: 0009-7322
Link: http://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00003017-199903160-00004
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: ovid00003017-199903160-00004
title: Tenascin-C Is Expressed in Macrophage-Rich Human Coronary Atherosclerotic Plaque
format: Article
creator:
  • Wallner, Kurt
  • Li, Chen
  • Shah, Prediman K.
  • Fishbein, Michael C.
  • Forrester, James S.
  • Kaul, Sanjay
  • Sharifi, Behrooz G.
subjects:
  • Medicine
  • Anatomy & Physiology
ispartof: Circulation, 1999, Vol.99(10), pp.1284-1289
description: BACKGROUND: Tenascin is a large extracellular matrix glycoprotein generally found in adult tissues undergoing active remodeling such as healing wounds and tumors. To determine the potential role of tenascin-C (TN-C) in the pathophysiology of atherosclerosis, we investigated the pattern of expression of TN-C in human coronary atherosclerotic plaques.Methods and Results-Immunohistochemical staining and in situ hybridization demonstrated minimal and random expression of TN-C in fibrotic but lipid-poor atherosclerotic plaques. In contrast, all plaques with an organized lipid core or ruptured intimal surface strongly expressed TN-C, which was preferentially concentrated around the lipid core, shoulder regions, and ruptured area of the plaques but not in the fibrous cap. TN-C was not detected in normal arterial tissue. To identify the cellular source of TN-C, the plaques were stained with smooth muscle cell- and macrophage-specific antibodies. TN-C expression correlated with the infiltration of macrophages. Northern blot and immunoprecipitation analysis showed that macrophages expressed 7.0-kb TN-C mRNA and 220-kDa protein. Reverse transcription-polymerase chain reaction of total RNA derived from macrophages showed that they express the small isoform of TN-C. Zymogram analysis revealed that macrophages markedly increased MMP-9 expression. CONCLUSIONS: This study demonstrates that the level of TN-C expression correlates with the degree of inflammation present, not with plaque size. In addition, cultured macrophages have the capacity to express the TN-C gene. These findings suggest the significance of macrophages in the remodeling of atherosclerotic plaque matrix composition. (Circulation. 1999;99:1284-1289.)
language:
source:
identifier: ISSN: 0009-7322
fulltext: fulltext
issn:
  • 0009-7322
  • 00097322
url: Link


@attributes
ID1658731421
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid00003017-199903160-00004
sourceidovid
recordidTN_ovid00003017-199903160-00004
sourceformatXML
sourcesystemPC
pqid212681044
display
typearticle
titleTenascin-C Is Expressed in Macrophage-Rich Human Coronary Atherosclerotic Plaque
creatorWallner, Kurt ; Li, Chen ; Shah, Prediman K. ; Fishbein, Michael C. ; Forrester, James S. ; Kaul, Sanjay ; Sharifi, Behrooz G.
ispartofCirculation, 1999, Vol.99(10), pp.1284-1289
identifierISSN: 0009-7322
descriptionBACKGROUND: Tenascin is a large extracellular matrix glycoprotein generally found in adult tissues undergoing active remodeling such as healing wounds and tumors. To determine the potential role of tenascin-C (TN-C) in the pathophysiology of atherosclerosis, we investigated the pattern of expression of TN-C in human coronary atherosclerotic plaques.Methods and Results-Immunohistochemical staining and in situ hybridization demonstrated minimal and random expression of TN-C in fibrotic but lipid-poor atherosclerotic plaques. In contrast, all plaques with an organized lipid core or ruptured intimal surface strongly expressed TN-C, which was preferentially concentrated around the lipid core, shoulder regions, and ruptured area of the plaques but not in the fibrous cap. TN-C was not detected in normal arterial tissue. To identify the cellular source of TN-C, the plaques were stained with smooth muscle cell- and macrophage-specific antibodies. TN-C expression correlated with the infiltration of macrophages. Northern blot and immunoprecipitation analysis showed that macrophages expressed 7.0-kb TN-C mRNA and 220-kDa protein. Reverse transcription-polymerase chain reaction of total RNA derived from macrophages showed that they express the small isoform of TN-C. Zymogram analysis revealed that macrophages markedly increased MMP-9 expression. CONCLUSIONS: This study demonstrates that the level of TN-C expression correlates with the degree of inflammation present, not with plaque size. In addition, cultured macrophages have the capacity to express the TN-C gene. These findings suggest the significance of macrophages in the remodeling of atherosclerotic plaque matrix composition. (Circulation. 1999;99:1284-1289.)
source
subjectMedicine ; Anatomy & Physiology;
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00003017-199903160-00004$$EView_record_at_Wolters_Kluwer_Health_Ovid_Technologies
openurlfulltext$$Topenurlfull_article
addlink$$Uhttp://exlibris-pub.s3.amazonaws.com/aboutOvid.html$$EView_Wolters_Kluwer_Health,_Lippincott,_Williams_&_Wilkins_Copyright_Statement
search
creatorcontrib
0Wallner, Kurt
1Li, Chen
2Shah, Prediman K
3Fishbein, Michael C
4Forrester, James S
5Kaul, Sanjay
6Sharifi, Behrooz G
titleTenascin-C Is Expressed in Macrophage-Rich Human Coronary Atherosclerotic Plaque
descriptionBACKGROUND: Tenascin is a large extracellular matrix glycoprotein generally found in adult tissues undergoing active remodeling such as healing wounds and tumors. To determine the potential role of tenascin-C (TN-C) in the pathophysiology of atherosclerosis, we investigated the pattern of expression of TN-C in human coronary atherosclerotic plaques.Methods and Results-Immunohistochemical staining and in situ hybridization demonstrated minimal and random expression of TN-C in fibrotic but lipid-poor atherosclerotic plaques. In contrast, all plaques with an organized lipid core or ruptured intimal surface strongly expressed TN-C, which was preferentially concentrated around the lipid core, shoulder regions, and ruptured area of the plaques but not in the fibrous cap. TN-C was not detected in normal arterial tissue. To identify the cellular source of TN-C, the plaques were stained with smooth muscle cell- and macrophage-specific antibodies. TN-C expression correlated with the infiltration of macrophages. Northern blot and immunoprecipitation analysis showed that macrophages expressed 7.0-kb TN-C mRNA and 220-kDa protein. Reverse transcription-polymerase chain reaction of total RNA derived from macrophages showed that they express the small isoform of TN-C. Zymogram analysis revealed that macrophages markedly increased MMP-9 expression. CONCLUSIONS: This study demonstrates that the level of TN-C expression correlates with the degree of inflammation present, not with plaque size. In addition, cultured macrophages have the capacity to express the TN-C gene. These findings suggest the significance of macrophages in the remodeling of atherosclerotic plaque matrix composition. (Circulation. 1999;99:1284-1289.)
general
0© 1999 American Heart Association, Inc.
1Lippincott Williams & Wilkins - Journals
sourceidovid
recordidovid00003017-199903160-00004
issn
00009-7322
100097322
rsrctypearticle
creationdate1999
addtitleCirculation
searchscopeovid
scopeovid
lsr30VSR-Enriched:[pqid, subject, eissn, date, doi]
sort
titleTenascin-C Is Expressed in Macrophage-Rich Human Coronary Atherosclerotic Plaque
authorWallner, Kurt ; Li, Chen ; Shah, Prediman K. ; Fishbein, Michael C. ; Forrester, James S. ; Kaul, Sanjay ; Sharifi, Behrooz G.
creationdate19990300
facets
frbrgroupid7931015355036483108
frbrtype5
newrecords20170719
creationdate1999
collectionLippincott Williams & Wilkins Journals (Wolters Kluwer Health)
prefilterarticles
rsrctypearticles
creatorcontrib
0Wallner, Kurt
1Li, Chen
2Shah, Prediman K.
3Fishbein, Michael C.
4Forrester, James S.
5Kaul, Sanjay
6Sharifi, Behrooz G.
jtitleCirculation
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Wallner
1Li
2Shah
3Fishbein
4Forrester
5Kaul
6Sharifi
aufirst
0Kurt
1Chen
2Prediman K.
3Michael C.
4James S.
5Sanjay
6Behrooz G.
au
0Wallner, Kurt
1Li, Chen
2Shah, Prediman K.
3Fishbein, Michael C.
4Forrester, James S.
5Kaul, Sanjay
6Sharifi, Behrooz G.
atitleTenascin-C Is Expressed in Macrophage-Rich Human Coronary Atherosclerotic Plaque
jtitleCirculation
risdate199903
volume99
issue10
spage1284
epage1289
pages1284-1289
issn0009-7322
formatjournal
genrearticle
ristypeJOUR
abstractBACKGROUND: Tenascin is a large extracellular matrix glycoprotein generally found in adult tissues undergoing active remodeling such as healing wounds and tumors. To determine the potential role of tenascin-C (TN-C) in the pathophysiology of atherosclerosis, we investigated the pattern of expression of TN-C in human coronary atherosclerotic plaques.Methods and Results-Immunohistochemical staining and in situ hybridization demonstrated minimal and random expression of TN-C in fibrotic but lipid-poor atherosclerotic plaques. In contrast, all plaques with an organized lipid core or ruptured intimal surface strongly expressed TN-C, which was preferentially concentrated around the lipid core, shoulder regions, and ruptured area of the plaques but not in the fibrous cap. TN-C was not detected in normal arterial tissue. To identify the cellular source of TN-C, the plaques were stained with smooth muscle cell- and macrophage-specific antibodies. TN-C expression correlated with the infiltration of macrophages. Northern blot and immunoprecipitation analysis showed that macrophages expressed 7.0-kb TN-C mRNA and 220-kDa protein. Reverse transcription-polymerase chain reaction of total RNA derived from macrophages showed that they express the small isoform of TN-C. Zymogram analysis revealed that macrophages markedly increased MMP-9 expression. CONCLUSIONS: This study demonstrates that the level of TN-C expression correlates with the degree of inflammation present, not with plaque size. In addition, cultured macrophages have the capacity to express the TN-C gene. These findings suggest the significance of macrophages in the remodeling of atherosclerotic plaque matrix composition. (Circulation. 1999;99:1284-1289.)
pub© 1999 American Heart Association, Inc.
doi10.1161/01.CIR.99.10.1284
eissn15244539
date1999-03-16