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Changes of glucocorticoid receptor mRNA expression in basolateral amygdale-kindled rats

BACKGROUND: Glucocorticoid receptor (GR) is believed to be a major factor in brain maturation and in modulation of a series of brain activity. Hippocampal neurons are abundant in glucocorticoid receptor, and there is significant change in GR expression under certain pathological state. Epilepsy is a... Full description

Journal Title: Chinese Medical Journal 2011, Vol.124(17), pp.2622-2627
Main Author: Bao, Guan-Shui
Other Authors: Chen, Xu-Qin , Hua, Yin , Wang, Zhe-Dong , Liu, Zhen-Guo
Format: Electronic Article Electronic Article
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ID: ISSN: 0366-6999 ; DOI: 10.3760/cma.j.issn.0366-6999.2011.17.011
Link: http://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00029330-201109010-00011
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title: Changes of glucocorticoid receptor mRNA expression in basolateral amygdale-kindled rats
format: Article
creator:
  • Bao, Guan-Shui
  • Chen, Xu-Qin
  • Hua, Yin
  • Wang, Zhe-Dong
  • Liu, Zhen-Guo
subjects:
  • Medicine
ispartof: Chinese Medical Journal, 2011, Vol.124(17), pp.2622-2627
description: BACKGROUND: Glucocorticoid receptor (GR) is believed to be a major factor in brain maturation and in modulation of a series of brain activity. Hippocampal neurons are abundant in glucocorticoid receptor, and there is significant change in GR expression under certain pathological state. Epilepsy is a special pathological state of the central nervous system. This study aimed to explore the role of GR in epilepsy by observing the change and functions of GR in hippocampus with a basolateral amygdale-electrical kindled rat epilepsy model. METHODS: Firstly, we established the basolateral amygdale-electrical kindled rat epilepsy model. Then GR mRNA expression in the hippocampus was assayed by semi-quantitative reverse transcription-PCR in this experiment. In addition, the processes of epileptic seizures were observed and electroencephalograms were recorded. One-way analysis of variance (ANOVA) was employed for comparing means of multiple groups, followed Fisher’s least significant difference (LSD) for paired comparison. RESULTS: The rats were successfully kindled after an average of (13.50±3.99) times electrical stimulation, in which it was showed that GR mRNA expression reduced obviously as compared with the control group and the sham groups (P
language:
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identifier: ISSN: 0366-6999 ; DOI: 10.3760/cma.j.issn.0366-6999.2011.17.011
fulltext: fulltext
issn:
  • 0366-6999
  • 03666999
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titleChanges of glucocorticoid receptor mRNA expression in basolateral amygdale-kindled rats
creatorBao, Guan-Shui ; Chen, Xu-Qin ; Hua, Yin ; Wang, Zhe-Dong ; Liu, Zhen-Guo
ispartofChinese Medical Journal, 2011, Vol.124(17), pp.2622-2627
identifierISSN: 0366-6999 ; DOI: 10.3760/cma.j.issn.0366-6999.2011.17.011
descriptionBACKGROUND: Glucocorticoid receptor (GR) is believed to be a major factor in brain maturation and in modulation of a series of brain activity. Hippocampal neurons are abundant in glucocorticoid receptor, and there is significant change in GR expression under certain pathological state. Epilepsy is a special pathological state of the central nervous system. This study aimed to explore the role of GR in epilepsy by observing the change and functions of GR in hippocampus with a basolateral amygdale-electrical kindled rat epilepsy model. METHODS: Firstly, we established the basolateral amygdale-electrical kindled rat epilepsy model. Then GR mRNA expression in the hippocampus was assayed by semi-quantitative reverse transcription-PCR in this experiment. In addition, the processes of epileptic seizures were observed and electroencephalograms were recorded. One-way analysis of variance (ANOVA) was employed for comparing means of multiple groups, followed Fisher’s least significant difference (LSD) for paired comparison. RESULTS: The rats were successfully kindled after an average of (13.50±3.99) times electrical stimulation, in which it was showed that GR mRNA expression reduced obviously as compared with the control group and the sham groups (P <0.001). The down-regulation of GR mRNA expression was abated or reversed by some anti-epilepsy drugs (P <0.001 compared with the epilepsy group), accompanied by attenuation of seizures and improvement of electroencephalograms. CONCLUSIONS: Down-regulation of hippocampal GR mRNA expression may be related to the kindling. Anti-epilepsy drugs exposure can retard this change.
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descriptionBACKGROUND: Glucocorticoid receptor (GR) is believed to be a major factor in brain maturation and in modulation of a series of brain activity. Hippocampal neurons are abundant in glucocorticoid receptor, and there is significant change in GR expression under certain pathological state. Epilepsy is a special pathological state of the central nervous system. This study aimed to explore the role of GR in epilepsy by observing the change and functions of GR in hippocampus with a basolateral amygdale-electrical kindled rat epilepsy model. METHODS: Firstly, we established the basolateral amygdale-electrical kindled rat epilepsy model. Then GR mRNA expression in the hippocampus was assayed by semi-quantitative reverse transcription-PCR in this experiment. In addition, the processes of epileptic seizures were observed and electroencephalograms were recorded. One-way analysis of variance (ANOVA) was employed for comparing means of multiple groups, followed Fisher’s least significant difference (LSD) for paired comparison. RESULTS: The rats were successfully kindled after an average of (13.50±3.99) times electrical stimulation, in which it was showed that GR mRNA expression reduced obviously as compared with the control group and the sham groups (P <0.001). The down-regulation of GR mRNA expression was abated or reversed by some anti-epilepsy drugs (P <0.001 compared with the epilepsy group), accompanied by attenuation of seizures and improvement of electroencephalograms. CONCLUSIONS: Down-regulation of hippocampal GR mRNA expression may be related to the kindling. Anti-epilepsy drugs exposure can retard this change.
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abstractBACKGROUND: Glucocorticoid receptor (GR) is believed to be a major factor in brain maturation and in modulation of a series of brain activity. Hippocampal neurons are abundant in glucocorticoid receptor, and there is significant change in GR expression under certain pathological state. Epilepsy is a special pathological state of the central nervous system. This study aimed to explore the role of GR in epilepsy by observing the change and functions of GR in hippocampus with a basolateral amygdale-electrical kindled rat epilepsy model. METHODS: Firstly, we established the basolateral amygdale-electrical kindled rat epilepsy model. Then GR mRNA expression in the hippocampus was assayed by semi-quantitative reverse transcription-PCR in this experiment. In addition, the processes of epileptic seizures were observed and electroencephalograms were recorded. One-way analysis of variance (ANOVA) was employed for comparing means of multiple groups, followed Fisher’s least significant difference (LSD) for paired comparison. RESULTS: The rats were successfully kindled after an average of (13.50±3.99) times electrical stimulation, in which it was showed that GR mRNA expression reduced obviously as compared with the control group and the sham groups (P <0.001). The down-regulation of GR mRNA expression was abated or reversed by some anti-epilepsy drugs (P <0.001 compared with the epilepsy group), accompanied by attenuation of seizures and improvement of electroencephalograms. CONCLUSIONS: Down-regulation of hippocampal GR mRNA expression may be related to the kindling. Anti-epilepsy drugs exposure can retard this change.
pub© 2011 Chinese Medical Association
doi10.3760/cma.j.issn.0366-6999.2011.17.011
date2011-09