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Quantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging

Multimodal label-free molecular imaging allows 3D phenotypic characterisation and quantitation of bioengineered cartilage non-invasively and non-destructively. The unique properties of skeletal stem cells have attracted significant attention in the development of strategies for skeletal regeneration... Full description

Journal Title: Integrative Biology 2018, Vol. 10(10), pp.635-645
Main Author: Costa Moura, Catarina
Other Authors: Lanham, Stuart A , Monfort, Tual , Bourdakos, Konstantinos N , Tare, Rahul S , Oreffo, Richard O. C , Mahajan, Sumeet
Format: Electronic Article Electronic Article
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ID: E-ISSN: 1757-9708 ; DOI: 10.1039/c8ib00050f
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recordid: oxford10.1039/c8ib00050f
title: Quantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging
format: Article
creator:
  • Costa Moura, Catarina
  • Lanham, Stuart A
  • Monfort, Tual
  • Bourdakos, Konstantinos N
  • Tare, Rahul S
  • Oreffo, Richard O. C
  • Mahajan, Sumeet
subjects:
  • Imaging Techniques
  • Raman Spectra
  • Cartilage
  • Regeneration
  • Second Harmonic Generation
  • Coherent Scattering
  • Nondestructive Testing
  • Fluorescence
  • Interrogation
  • Fluorescence
  • Collagen
  • Regeneration (Physiology)
  • Stem Cells
  • Stem Cells
  • Cartilage
  • Regeneration
  • Bioengineering
  • Tissue Engineering
  • Tissue Engineering
  • Stem Cells
ispartof: Integrative Biology, 2018, Vol. 10(10), pp.635-645
description: Multimodal label-free molecular imaging allows 3D phenotypic characterisation and quantitation of bioengineered cartilage non-invasively and non-destructively. The unique properties of skeletal stem cells have attracted significant attention in the development of strategies for skeletal regeneration. However, there remains a crucial unmet need to develop quantitative tools to elucidate skeletal cell development and monitor the formation of regenerated tissues using non-destructive techniques in 3D. Label-free methods such as coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two-photon excited auto-fluorescence (TPEAF) microscopy are minimally invasive, non-destructive, and present new powerful alternatives to conventional imaging techniques. Here we report a combination of these techniques in a single multimodal system for the temporal assessment of cartilage formation by human skeletal cells. The evaluation of bioengineered cartilage, with a new parameter measuring the amount of collagen per cell, collagen fibre structure and chondrocyte distribution, was performed using the 3D non-destructive platform. Such 3D label-free temporal quantification paves the way for tracking skeletal cell development in real-time and offers a paradigm shift in tissue engineering and regenerative medicine applications.
language:
source:
identifier: E-ISSN: 1757-9708 ; DOI: 10.1039/c8ib00050f
fulltext: fulltext
issn:
  • 1757-9708
  • 17579708
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titleQuantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging
creatorCosta Moura, Catarina ; Lanham, Stuart A ; Monfort, Tual ; Bourdakos, Konstantinos N ; Tare, Rahul S ; Oreffo, Richard O. C ; Mahajan, Sumeet
ispartofIntegrative Biology, 2018, Vol. 10(10), pp.635-645
identifierE-ISSN: 1757-9708 ; DOI: 10.1039/c8ib00050f
descriptionMultimodal label-free molecular imaging allows 3D phenotypic characterisation and quantitation of bioengineered cartilage non-invasively and non-destructively. The unique properties of skeletal stem cells have attracted significant attention in the development of strategies for skeletal regeneration. However, there remains a crucial unmet need to develop quantitative tools to elucidate skeletal cell development and monitor the formation of regenerated tissues using non-destructive techniques in 3D. Label-free methods such as coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two-photon excited auto-fluorescence (TPEAF) microscopy are minimally invasive, non-destructive, and present new powerful alternatives to conventional imaging techniques. Here we report a combination of these techniques in a single multimodal system for the temporal assessment of cartilage formation by human skeletal cells. The evaluation of bioengineered cartilage, with a new parameter measuring the amount of collagen per cell, collagen fibre structure and chondrocyte distribution, was performed using the 3D non-destructive platform. Such 3D label-free temporal quantification paves the way for tracking skeletal cell development in real-time and offers a paradigm shift in tissue engineering and regenerative medicine applications.
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subjectImaging Techniques ; Raman Spectra ; Cartilage ; Regeneration ; Second Harmonic Generation ; Coherent Scattering ; Nondestructive Testing ; Fluorescence ; Interrogation ; Fluorescence ; Collagen ; Regeneration (Physiology) ; Stem Cells ; Stem Cells ; Cartilage ; Regeneration ; Bioengineering ; Tissue Engineering ; Tissue Engineering ; Stem Cells;
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titleQuantitative temporal interrogation in 3D of bioengineered human cartilage using multimodal label-free imaging
authorCosta Moura, Catarina ; Lanham, Stuart A ; Monfort, Tual ; Bourdakos, Konstantinos N ; Tare, Rahul S ; Oreffo, Richard O. C ; Mahajan, Sumeet
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abstractMultimodal label-free molecular imaging allows 3D phenotypic characterisation and quantitation of bioengineered cartilage non-invasively and non-destructively. The unique properties of skeletal stem cells have attracted significant attention in the development of strategies for skeletal regeneration. However, there remains a crucial unmet need to develop quantitative tools to elucidate skeletal cell development and monitor the formation of regenerated tissues using non-destructive techniques in 3D. Label-free methods such as coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two-photon excited auto-fluorescence (TPEAF) microscopy are minimally invasive, non-destructive, and present new powerful alternatives to conventional imaging techniques. Here we report a combination of these techniques in a single multimodal system for the temporal assessment of cartilage formation by human skeletal cells. The evaluation of bioengineered cartilage, with a new parameter measuring the amount of collagen per cell, collagen fibre structure and chondrocyte distribution, was performed using the 3D non-destructive platform. Such 3D label-free temporal quantification paves the way for tracking skeletal cell development in real-time and offers a paradigm shift in tissue engineering and regenerative medicine applications.
pubOxford University Press
doi10.1039/c8ib00050f
issn17579694
date2018-10