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Serratamolide is a Hemolytic Factor Produced by Serratia marcescens (New Serratia marcescens Hemolysin)

Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A g... Full description

Journal Title: PLoS ONE 2012, Vol.7(5), p.e36398
Main Author: Shanks, Robert M. Q
Other Authors: Stella, Nicholas A , Lahr, Roni M , Wang, Shaoru , Veverka, Tara I , Kowalski, Regis P , Liu, Xinyu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0036398
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recordid: plos10.1371/journal.pone.0036398
title: Serratamolide is a Hemolytic Factor Produced by Serratia marcescens (New Serratia marcescens Hemolysin)
format: Article
creator:
  • Shanks, Robert M. Q
  • Stella, Nicholas A
  • Lahr, Roni M
  • Wang, Shaoru
  • Veverka, Tara I
  • Kowalski, Regis P
  • Liu, Xinyu
subjects:
  • Research Article
  • Biology
  • Chemistry
  • Medicine
  • Genetics And Genomics
  • Immunology
  • Chemistry
  • Infectious Diseases
  • Ophthalmology
  • Microbiology
  • Biochemistry
ispartof: PLoS ONE, 2012, Vol.7(5), p.e36398
description: Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0036398
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


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titleSerratamolide is a Hemolytic Factor Produced by Serratia marcescens (New Serratia marcescens Hemolysin)
creatorShanks, Robert M. Q ; Stella, Nicholas A ; Lahr, Roni M ; Wang, Shaoru ; Veverka, Tara I ; Kowalski, Regis P ; Liu, Xinyu
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0036398
subjectResearch Article ; Biology ; Chemistry ; Medicine ; Genetics And Genomics ; Immunology ; Chemistry ; Infectious Diseases ; Ophthalmology ; Microbiology ; Biochemistry
descriptionSerratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use.
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titleSerratamolide is a Hemolytic Factor Produced by Serratia marcescens (New Serratia marcescens Hemolysin)
descriptionSerratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use.
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abstractSerratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use.
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pubPublic Library of Science
doi10.1371/journal.pone.0036398
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date2012-05-16