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Identification of Genes Required for Nonhost Resistance to Xanthomonas oryzae pv. oryzae Reveals Novel Signaling Components (Genes Required for Nonhost Resistance)

Nonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosyst... Full description

Journal Title: 2012 Vol.7(8), p.e42796
Main Author: Li, Wen
Other Authors: Xu, You-Ping , Zhang, Zhi-Xin , Cao, Wen-Yuan , Li, Fei , Zhou, Xueping , Chen, Gong-You , Cai, Xin-Zhong
Format: Electronic Article Electronic Article
Language: English
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ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0042796
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recordid: plos10.1371/journal.pone.0042796
title: Identification of Genes Required for Nonhost Resistance to Xanthomonas oryzae pv. oryzae Reveals Novel Signaling Components (Genes Required for Nonhost Resistance)
format: Article
creator:
  • Li, Wen
  • Xu, You-Ping
  • Zhang, Zhi-Xin
  • Cao, Wen-Yuan
  • Li, Fei
  • Zhou, Xueping
  • Chen, Gong-You
  • Cai, Xin-Zhong
subjects:
  • Research Article
  • Agriculture
  • Biology
  • Genetics And Genomics
  • Plant Biology
  • Microbiology
ispartof: 2012, Vol.7(8), p.e42796
description: Nonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo). ; Virus-induced gene silencing (VIGS) and an assay to detect the hypersensitive response (HR) were used to screen for genes required for nonhost resistance to Xoo in . When infiltrated with Xoo strain YN-1, plants exhibited a strong necrosis within 24 h and produced a large amount of HO in the infiltrated area. Expression of HR- and defense-related genes was induced, whereas bacterial numbers dramatically decreased during necrosis. VIGS of 45 (vr/f-licited) genes revealed identified seven genes required for nonhost resistance to Xoo in . The seven genes encoded a calreticulin protein (ACE35), an ERF transcriptional factor (ACE43), a novel Solanaceous protein (ACE80), a hydrolase (ACE117), a peroxidase (ACE175) and two proteins with unknown function (ACE95 and ACE112). The results indicate that oxidative burst and calcium-dependent signaling pathways play an important role in nonhost resistance to Xoo. VIGS analysis further revealed that , , and , but not the other three genes, interfered with the Cf-4/Avr4-dependent HR. ; plants inoculated with Xoo respond by rapidly eliciting an HR and nonhost resistance. The oxidative burst and other signaling pathways are pivotal in Xoo- nonhost resistance, and genes involved in this response partially overlap with those involved in Cf/Avr4-dependent HR. The seven genes required for -mediated resistance to Xoo provide a basis for further dissecting the molecular mechanism of nonhost resistance.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0042796
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


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titleIdentification of Genes Required for Nonhost Resistance to Xanthomonas oryzae pv. oryzae Reveals Novel Signaling Components (Genes Required for Nonhost Resistance)
creatorLi, Wen ; Xu, You-Ping ; Zhang, Zhi-Xin ; Cao, Wen-Yuan ; Li, Fei ; Zhou, Xueping ; Chen, Gong-You ; Cai, Xin-Zhong
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descriptionNonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo). ; Virus-induced gene silencing (VIGS) and an assay to detect the hypersensitive response (HR) were used to screen for genes required for nonhost resistance to Xoo in . When infiltrated with Xoo strain YN-1, plants exhibited a strong necrosis within 24 h and produced a large amount of HO in the infiltrated area. Expression of HR- and defense-related genes was induced, whereas bacterial numbers dramatically decreased during necrosis. VIGS of 45 (vr/f-licited) genes revealed identified seven genes required for nonhost resistance to Xoo in . The seven genes encoded a calreticulin protein (ACE35), an ERF transcriptional factor (ACE43), a novel Solanaceous protein (ACE80), a hydrolase (ACE117), a peroxidase (ACE175) and two proteins with unknown function (ACE95 and ACE112). The results indicate that oxidative burst and calcium-dependent signaling pathways play an important role in nonhost resistance to Xoo. VIGS analysis further revealed that , , and , but not the other three genes, interfered with the Cf-4/Avr4-dependent HR. ; plants inoculated with Xoo respond by rapidly eliciting an HR and nonhost resistance. The oxidative burst and other signaling pathways are pivotal in Xoo- nonhost resistance, and genes involved in this response partially overlap with those involved in Cf/Avr4-dependent HR. The seven genes required for -mediated resistance to Xoo provide a basis for further dissecting the molecular mechanism of nonhost resistance.
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titleIdentification of Genes Required for Nonhost Resistance to Xanthomonas oryzae pv. oryzae Reveals Novel Signaling Components (Genes Required for Nonhost Resistance)
descriptionNonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo). ; Virus-induced gene silencing (VIGS) and an assay to detect the hypersensitive response (HR) were used to screen for genes required for nonhost resistance to Xoo in . When infiltrated with Xoo strain YN-1, plants exhibited a strong necrosis within 24 h and produced a large amount of HO in the infiltrated area. Expression of HR- and defense-related genes was induced, whereas bacterial numbers dramatically decreased during necrosis. VIGS of 45 (vr/f-licited) genes revealed identified seven genes required for nonhost resistance to Xoo in . The seven genes encoded a calreticulin protein (ACE35), an ERF transcriptional factor (ACE43), a novel Solanaceous protein (ACE80), a hydrolase (ACE117), a peroxidase (ACE175) and two proteins with unknown function (ACE95 and ACE112). The results indicate that oxidative burst and calcium-dependent signaling pathways play an important role in nonhost resistance to Xoo. VIGS analysis further revealed that , , and , but not the other three genes, interfered with the Cf-4/Avr4-dependent HR. ; plants inoculated with Xoo respond by rapidly eliciting an HR and nonhost resistance. The oxidative burst and other signaling pathways are pivotal in Xoo- nonhost resistance, and genes involved in this response partially overlap with those involved in Cf/Avr4-dependent HR. The seven genes required for -mediated resistance to Xoo provide a basis for further dissecting the molecular mechanism of nonhost resistance.
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abstractNonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo). ; Virus-induced gene silencing (VIGS) and an assay to detect the hypersensitive response (HR) were used to screen for genes required for nonhost resistance to Xoo in . When infiltrated with Xoo strain YN-1, plants exhibited a strong necrosis within 24 h and produced a large amount of HO in the infiltrated area. Expression of HR- and defense-related genes was induced, whereas bacterial numbers dramatically decreased during necrosis. VIGS of 45 (vr/f-licited) genes revealed identified seven genes required for nonhost resistance to Xoo in . The seven genes encoded a calreticulin protein (ACE35), an ERF transcriptional factor (ACE43), a novel Solanaceous protein (ACE80), a hydrolase (ACE117), a peroxidase (ACE175) and two proteins with unknown function (ACE95 and ACE112). The results indicate that oxidative burst and calcium-dependent signaling pathways play an important role in nonhost resistance to Xoo. VIGS analysis further revealed that , , and , but not the other three genes, interfered with the Cf-4/Avr4-dependent HR. ; plants inoculated with Xoo respond by rapidly eliciting an HR and nonhost resistance. The oxidative burst and other signaling pathways are pivotal in Xoo- nonhost resistance, and genes involved in this response partially overlap with those involved in Cf/Avr4-dependent HR. The seven genes required for -mediated resistance to Xoo provide a basis for further dissecting the molecular mechanism of nonhost resistance.
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doi10.1371/journal.pone.0042796
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date2012-08-13