schliessen

Filtern

 

Bibliotheken

Immunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice (H5N1 Protection by Epitope-Based Vaccine)

Whole-virus vaccines, including inactivated or live-attenuated influenza vaccines, have been conventionally developed and supported as a prophylaxis. These currently available virus-based influenza vaccines are widely used in the clinic, but the vaccine production takes a long time and a huge number... Full description

Journal Title: 2012 Vol.7(11), p.e48750
Main Author: Rhee, Jae Won
Other Authors: Kim, Dongbum , Park, Byung Kwon , Kwon, Sanghoon , Cho, Sunhee , Lee, Ilseob , Park, Man-Seong , Seo, Jae-Nam , Kim, Yong-Sun , Choi, Hong Seok , Lee, Younghee , Kwon, Hyung-Joo
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0048750
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: plos10.1371/journal.pone.0048750
title: Immunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice (H5N1 Protection by Epitope-Based Vaccine)
format: Article
creator:
  • Rhee, Jae Won
  • Kim, Dongbum
  • Park, Byung Kwon
  • Kwon, Sanghoon
  • Cho, Sunhee
  • Lee, Ilseob
  • Park, Man-Seong
  • Seo, Jae-Nam
  • Kim, Yong-Sun
  • Choi, Hong Seok
  • Lee, Younghee
  • Kwon, Hyung-Joo
subjects:
  • Research Article
  • Biology
  • Medicine
  • Virology
  • Immunology
  • Infectious Diseases
ispartof: 2012, Vol.7(11), p.e48750
description: Whole-virus vaccines, including inactivated or live-attenuated influenza vaccines, have been conventionally developed and supported as a prophylaxis. These currently available virus-based influenza vaccines are widely used in the clinic, but the vaccine production takes a long time and a huge number of embryonated chicken eggs. To overcome the imperfection of egg-based influenza vaccines, epitope-based peptide vaccines have been studied as an alternative approach. Here, we formulated an efficacious peptide vaccine without carriers using phosphodiester CpG-DNA and a special liposome complex. Potential epitope peptides predicted from the hemagglutinin (HA) protein of the H5N1 A/Viet Nam/1203/2004 strain (NCBI database, AAW80717) were used to immunize mice along with phosphodiester CpG-DNA co-encapsulated in a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS) complex (Lipoplex(O)) without carriers. We identified a B cell epitope peptide (hH5N1 HA233 epitope, 14 amino acids) that can potently induce epitope-specific antibodies. Furthermore, immunization with a complex of the B cell epitope and Lipoplex(O) completely protects mice challenged with a lethal dose of recombinant H5N1 virus. These results suggest that our improved peptide vaccine technology can be promptly applied to vaccine development against pandemic influenza. Furthermore our results suggest that potent epitopes, which cannot be easily found using proteins or a virus as an antigen, can be screened when we use a complex of peptide epitopes and Lipoplex(O).
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0048750
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID655277292
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1371/journal.pone.0048750
sourceidplos
recordidTN_plos10.1371/journal.pone.0048750
sourcesystemOther
pqid1326738040
galeid477091749
display
typearticle
titleImmunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice (H5N1 Protection by Epitope-Based Vaccine)
creatorRhee, Jae Won ; Kim, Dongbum ; Park, Byung Kwon ; Kwon, Sanghoon ; Cho, Sunhee ; Lee, Ilseob ; Park, Man-Seong ; Seo, Jae-Nam ; Kim, Yong-Sun ; Choi, Hong Seok ; Lee, Younghee ; Kwon, Hyung-Joo
contributorLu, Shan (Editor)
ispartof2012, Vol.7(11), p.e48750
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0048750
subjectResearch Article ; Biology ; Medicine ; Virology ; Immunology ; Infectious Diseases
descriptionWhole-virus vaccines, including inactivated or live-attenuated influenza vaccines, have been conventionally developed and supported as a prophylaxis. These currently available virus-based influenza vaccines are widely used in the clinic, but the vaccine production takes a long time and a huge number of embryonated chicken eggs. To overcome the imperfection of egg-based influenza vaccines, epitope-based peptide vaccines have been studied as an alternative approach. Here, we formulated an efficacious peptide vaccine without carriers using phosphodiester CpG-DNA and a special liposome complex. Potential epitope peptides predicted from the hemagglutinin (HA) protein of the H5N1 A/Viet Nam/1203/2004 strain (NCBI database, AAW80717) were used to immunize mice along with phosphodiester CpG-DNA co-encapsulated in a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS) complex (Lipoplex(O)) without carriers. We identified a B cell epitope peptide (hH5N1 HA233 epitope, 14 amino acids) that can potently induce epitope-specific antibodies. Furthermore, immunization with a complex of the B cell epitope and Lipoplex(O) completely protects mice challenged with a lethal dose of recombinant H5N1 virus. These results suggest that our improved peptide vaccine technology can be promptly applied to vaccine development against pandemic influenza. Furthermore our results suggest that potent epitopes, which cannot be easily found using proteins or a virus as an antigen, can be screened when we use a complex of peptide epitopes and Lipoplex(O).
languageeng
source
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Rhee, Jae Won
1Kim, Dongbum
2Park, Byung Kwon
3Kwon, Sanghoon
4Cho, Sunhee
5Lee, Ilseob
6Park, Man-Seong
7Seo, Jae-Nam
8Kim, Yong-Sun
9Choi, Hong Seok
10Lee, Younghee
11Kwon, Hyung-Joo
12Lu, Shan (Editor)
titleImmunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice (H5N1 Protection by Epitope-Based Vaccine)
descriptionWhole-virus vaccines, including inactivated or live-attenuated influenza vaccines, have been conventionally developed and supported as a prophylaxis. These currently available virus-based influenza vaccines are widely used in the clinic, but the vaccine production takes a long time and a huge number of embryonated chicken eggs. To overcome the imperfection of egg-based influenza vaccines, epitope-based peptide vaccines have been studied as an alternative approach. Here, we formulated an efficacious peptide vaccine without carriers using phosphodiester CpG-DNA and a special liposome complex. Potential epitope peptides predicted from the hemagglutinin (HA) protein of the H5N1 A/Viet Nam/1203/2004 strain (NCBI database, AAW80717) were used to immunize mice along with phosphodiester CpG-DNA co-encapsulated in a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS) complex (Lipoplex(O)) without carriers. We identified a B cell epitope peptide (hH5N1 HA233 epitope, 14 amino acids) that can potently induce epitope-specific antibodies. Furthermore, immunization with a complex of the B cell epitope and Lipoplex(O) completely protects mice challenged with a lethal dose of recombinant H5N1 virus. These results suggest that our improved peptide vaccine technology can be promptly applied to vaccine development against pandemic influenza. Furthermore our results suggest that potent epitopes, which cannot be easily found using proteins or a virus as an antigen, can be screened when we use a complex of peptide epitopes and Lipoplex(O).
subject
0Research Article
1Biology
2Medicine
3Virology
4Immunology
5Infectious Diseases
general
010.1371/journal.pone.0048750
1English
sourceidplos
recordidplos10.1371/journal.pone.0048750
issn
01932-6203
119326203
rsrctypearticle
creationdate2012
recordtypearticle
addtitleH5N1 Protection by Epitope-Based Vaccine
searchscopeplos
scopeplos
lsr30VSR-Enriched:[galeid, pqid]
sort
titleImmunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice (H5N1 Protection by Epitope-Based Vaccine)
authorRhee, Jae Won ; Kim, Dongbum ; Park, Byung Kwon ; Kwon, Sanghoon ; Cho, Sunhee ; Lee, Ilseob ; Park, Man-Seong ; Seo, Jae-Nam ; Kim, Yong-Sun ; Choi, Hong Seok ; Lee, Younghee ; Kwon, Hyung-Joo
creationdate20121107
facets
frbrgroupid8589167439357269082
frbrtype5
languageeng
creationdate2012
topic
0Research Article
1Biology
2Medicine
3Virology
4Immunology
5Infectious Diseases
collectionPLoS
prefilterarticles
rsrctypearticles
creatorcontrib
0Rhee, Jae Won
1Kim, Dongbum
2Park, Byung Kwon
3Kwon, Sanghoon
4Cho, Sunhee
5Lee, Ilseob
6Park, Man-Seong
7Seo, Jae-Nam
8Kim, Yong-Sun
9Choi, Hong Seok
10Lee, Younghee
11Kwon, Hyung-Joo
12Lu, Shan
toplevelpeer_reviewed
frbr
t2
k12012
k219326203
k310.1371/journal.pone.0048750
k47
k511
k648750
k8immunization with hemagglutinin derived synthetic peptide formulated with cpg dna liposome complex induced protection against lethal influenza virus infection in mice
k9immunizationwithahemnmice
k12immunizationwithahemagglu
k15jaewonrhee
k16rheejaewon
delivery
delcategoryRemote Search Resource
fulltextfulltext
ranking
booster11
booster21
pcg_typepublisher
addata
aulast
0Rhee
1Kim
2Park
3Kwon
4Cho
5Lee
6Seo
7Choi
8Lu
aufirst
0Jae Won
1Dongbum
2Byung Kwon
3Sanghoon
4Sunhee
5Ilseob
6Man-Seong
7Jae-Nam
8Yong-Sun
9Hong Seok
10Younghee
11Hyung-Joo
12Shan
au
0Rhee, Jae Won
1Kim, Dongbum
2Park, Byung Kwon
3Kwon, Sanghoon
4Cho, Sunhee
5Lee, Ilseob
6Park, Man-Seong
7Seo, Jae-Nam
8Kim, Yong-Sun
9Choi, Hong Seok
10Lee, Younghee
11Kwon, Hyung-Joo
addauLu, Shan
atitleImmunization with a Hemagglutinin-Derived Synthetic Peptide Formulated with a CpG-DNA-Liposome Complex Induced Protection against Lethal Influenza Virus Infection in Mice (H5N1 Protection by Epitope-Based Vaccine)
risdate20121107
volume7
issue11
spagee48750
pagese48750
eissn1932-6203
genrearticle
ristypeJOUR
abstractWhole-virus vaccines, including inactivated or live-attenuated influenza vaccines, have been conventionally developed and supported as a prophylaxis. These currently available virus-based influenza vaccines are widely used in the clinic, but the vaccine production takes a long time and a huge number of embryonated chicken eggs. To overcome the imperfection of egg-based influenza vaccines, epitope-based peptide vaccines have been studied as an alternative approach. Here, we formulated an efficacious peptide vaccine without carriers using phosphodiester CpG-DNA and a special liposome complex. Potential epitope peptides predicted from the hemagglutinin (HA) protein of the H5N1 A/Viet Nam/1203/2004 strain (NCBI database, AAW80717) were used to immunize mice along with phosphodiester CpG-DNA co-encapsulated in a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS) complex (Lipoplex(O)) without carriers. We identified a B cell epitope peptide (hH5N1 HA233 epitope, 14 amino acids) that can potently induce epitope-specific antibodies. Furthermore, immunization with a complex of the B cell epitope and Lipoplex(O) completely protects mice challenged with a lethal dose of recombinant H5N1 virus. These results suggest that our improved peptide vaccine technology can be promptly applied to vaccine development against pandemic influenza. Furthermore our results suggest that potent epitopes, which cannot be easily found using proteins or a virus as an antigen, can be screened when we use a complex of peptide epitopes and Lipoplex(O).
copSan Francisco, USA
pubPublic Library of Science
doi10.1371/journal.pone.0048750
oafree_for_read
date2012-11-07