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RNAi-Mediated Silencing of Paramyosin Expression in Trichinella spiralis Results in Impaired Viability of the Parasite (RNAi-Mediated Silencing of Paramyosin Expression)

Trichinella spiralis expresses paramyosin ( Ts -PMY) not only as a structural protein but also as an immunomodulatory protein to protect the worm from being attacked by host complement components. In this study, the functions of PMY in the viability and the growth development of T. spiralis were con... Full description

Journal Title: 2012 Vol.7(11), p.e49913
Main Author: Chen, Xiaoqin
Other Authors: Yang, Yaping , Yang, Jing , Zhang, Zhifei , Zhu, Xinping
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0049913
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recordid: plos10.1371/journal.pone.0049913
title: RNAi-Mediated Silencing of Paramyosin Expression in Trichinella spiralis Results in Impaired Viability of the Parasite (RNAi-Mediated Silencing of Paramyosin Expression)
format: Article
creator:
  • Chen, Xiaoqin
  • Yang, Yaping
  • Yang, Jing
  • Zhang, Zhifei
  • Zhu, Xinping
subjects:
  • Research Article
  • Biology
  • Medicine
  • Veterinary Science
  • Genetics And Genomics
  • Infectious Diseases
  • Microbiology
  • Molecular Biology
  • Biochemistry
ispartof: 2012, Vol.7(11), p.e49913
description: Trichinella spiralis expresses paramyosin ( Ts -PMY) not only as a structural protein but also as an immunomodulatory protein to protect the worm from being attacked by host complement components. In this study, the functions of PMY in the viability and the growth development of T. spiralis were confirmed at the first time by silencing the gene function with RNA interference technique. ; To understand its functions in the viability of the worm, we used RNA interference to silence the expression of mRNA and protein in the parasite. Significant silencing of mRNA expression in larval and adult was achieved by siRNA and dsRNA through soaking and electroporation. Electroporation of larvae with 8 µM siRNA1743 or 100 ng/µl dsRNA-PF3 resulted in 66.3% and 60.4% decrease in transcript and 52.0% and 64.7% decrease in -PMY protein expression, respectively, compared with larvae treated with irrelevant control siRNA or dsRNA. Larvae treated with siRNA1743 displayed significant reduction in molting (40.8%) and serious surface damage as detected with SYTOX fluorescent staining. Infection of mice with larvae electroporated with siRNA1743 resulted in 37.6% decrease in adult worm burden and 23.2% decrease in muscle larvae burden compared with mice infected with control siRNA-treated larvae. In addition, adult worms recovered from mice infected with siRNA-treated larvae released 24.8% less newborn larvae. ; It is the first time RNAi was used on to demonstrate that silencing PMY expression in significantly reduces the parasite’s viability and infectivity, further confirming that PMY plays an important role in the survival of and therefore is a promising target for vaccine development.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0049913
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
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titleRNAi-Mediated Silencing of Paramyosin Expression in Trichinella spiralis Results in Impaired Viability of the Parasite (RNAi-Mediated Silencing of Paramyosin Expression)
creatorChen, Xiaoqin ; Yang, Yaping ; Yang, Jing ; Zhang, Zhifei ; Zhu, Xinping
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descriptionTrichinella spiralis expresses paramyosin ( Ts -PMY) not only as a structural protein but also as an immunomodulatory protein to protect the worm from being attacked by host complement components. In this study, the functions of PMY in the viability and the growth development of T. spiralis were confirmed at the first time by silencing the gene function with RNA interference technique. ; To understand its functions in the viability of the worm, we used RNA interference to silence the expression of mRNA and protein in the parasite. Significant silencing of mRNA expression in larval and adult was achieved by siRNA and dsRNA through soaking and electroporation. Electroporation of larvae with 8 µM siRNA1743 or 100 ng/µl dsRNA-PF3 resulted in 66.3% and 60.4% decrease in transcript and 52.0% and 64.7% decrease in -PMY protein expression, respectively, compared with larvae treated with irrelevant control siRNA or dsRNA. Larvae treated with siRNA1743 displayed significant reduction in molting (40.8%) and serious surface damage as detected with SYTOX fluorescent staining. Infection of mice with larvae electroporated with siRNA1743 resulted in 37.6% decrease in adult worm burden and 23.2% decrease in muscle larvae burden compared with mice infected with control siRNA-treated larvae. In addition, adult worms recovered from mice infected with siRNA-treated larvae released 24.8% less newborn larvae. ; It is the first time RNAi was used on to demonstrate that silencing PMY expression in significantly reduces the parasite’s viability and infectivity, further confirming that PMY plays an important role in the survival of and therefore is a promising target for vaccine development.
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titleRNAi-Mediated Silencing of Paramyosin Expression in Trichinella spiralis Results in Impaired Viability of the Parasite (RNAi-Mediated Silencing of Paramyosin Expression)
descriptionTrichinella spiralis expresses paramyosin ( Ts -PMY) not only as a structural protein but also as an immunomodulatory protein to protect the worm from being attacked by host complement components. In this study, the functions of PMY in the viability and the growth development of T. spiralis were confirmed at the first time by silencing the gene function with RNA interference technique. ; To understand its functions in the viability of the worm, we used RNA interference to silence the expression of mRNA and protein in the parasite. Significant silencing of mRNA expression in larval and adult was achieved by siRNA and dsRNA through soaking and electroporation. Electroporation of larvae with 8 µM siRNA1743 or 100 ng/µl dsRNA-PF3 resulted in 66.3% and 60.4% decrease in transcript and 52.0% and 64.7% decrease in -PMY protein expression, respectively, compared with larvae treated with irrelevant control siRNA or dsRNA. Larvae treated with siRNA1743 displayed significant reduction in molting (40.8%) and serious surface damage as detected with SYTOX fluorescent staining. Infection of mice with larvae electroporated with siRNA1743 resulted in 37.6% decrease in adult worm burden and 23.2% decrease in muscle larvae burden compared with mice infected with control siRNA-treated larvae. In addition, adult worms recovered from mice infected with siRNA-treated larvae released 24.8% less newborn larvae. ; It is the first time RNAi was used on to demonstrate that silencing PMY expression in significantly reduces the parasite’s viability and infectivity, further confirming that PMY plays an important role in the survival of and therefore is a promising target for vaccine development.
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abstractTrichinella spiralis expresses paramyosin ( Ts -PMY) not only as a structural protein but also as an immunomodulatory protein to protect the worm from being attacked by host complement components. In this study, the functions of PMY in the viability and the growth development of T. spiralis were confirmed at the first time by silencing the gene function with RNA interference technique. ; To understand its functions in the viability of the worm, we used RNA interference to silence the expression of mRNA and protein in the parasite. Significant silencing of mRNA expression in larval and adult was achieved by siRNA and dsRNA through soaking and electroporation. Electroporation of larvae with 8 µM siRNA1743 or 100 ng/µl dsRNA-PF3 resulted in 66.3% and 60.4% decrease in transcript and 52.0% and 64.7% decrease in -PMY protein expression, respectively, compared with larvae treated with irrelevant control siRNA or dsRNA. Larvae treated with siRNA1743 displayed significant reduction in molting (40.8%) and serious surface damage as detected with SYTOX fluorescent staining. Infection of mice with larvae electroporated with siRNA1743 resulted in 37.6% decrease in adult worm burden and 23.2% decrease in muscle larvae burden compared with mice infected with control siRNA-treated larvae. In addition, adult worms recovered from mice infected with siRNA-treated larvae released 24.8% less newborn larvae. ; It is the first time RNAi was used on to demonstrate that silencing PMY expression in significantly reduces the parasite’s viability and infectivity, further confirming that PMY plays an important role in the survival of and therefore is a promising target for vaccine development.
copSan Francisco, USA
pubPublic Library of Science
doi10.1371/journal.pone.0049913
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date2012-11-21