schliessen

Filtern

 

Bibliotheken

Identification of Potential Serum Proteomic Biomarkers for Clear Cell Renal Cell Carcinoma (Serum Biomarkers Detection for Clear Cell Renal Cell Carcinoma)

To investigate discriminating protein patterns and serum biomarkers between clear cell renal cell carcinoma (ccRCC) patients and healthy controls, as well as between paired pre- and post-operative ccRCC patients. ; We used magnetic bead-based separation followed by matrix-assisted laser desorption i... Full description

Journal Title: 2014 Vol.9(11), p.e111364
Main Author: Yang, Juan
Other Authors: Yang, Jin , Gao, Yan , Zhao, Lingyu , Liu, Liying , Qin, Yannan , Wang, Xiaofei , Song, Tusheng , Huang, Chen
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0111364
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: plos10.1371/journal.pone.0111364
title: Identification of Potential Serum Proteomic Biomarkers for Clear Cell Renal Cell Carcinoma (Serum Biomarkers Detection for Clear Cell Renal Cell Carcinoma)
format: Article
creator:
  • Yang, Juan
  • Yang, Jin
  • Gao, Yan
  • Zhao, Lingyu
  • Liu, Liying
  • Qin, Yannan
  • Wang, Xiaofei
  • Song, Tusheng
  • Huang, Chen
subjects:
  • Research Article
  • Medicine And Health Sciences
ispartof: 2014, Vol.9(11), p.e111364
description: To investigate discriminating protein patterns and serum biomarkers between clear cell renal cell carcinoma (ccRCC) patients and healthy controls, as well as between paired pre- and post-operative ccRCC patients. ; We used magnetic bead-based separation followed by matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to identify patients with ccRCC. A total of 162 serum samples were analyzed in this study, among which there were 58 serum samples from ccRCC patients, 40 from additional paired pre- and post-operative ccRCC patients (n = 20), and 64 from healthy volunteers as healthy controls. ClinProTools software identified several distinct markers between ccRCC patients and healthy controls, as well as between pre- and post-operative patients. ; Patients with ccRCC could be identified with a mean sensitivity of 88.38% and a mean specificity of 91.67%. Of 67 m/z peaks that differed among the ccRCC, healthy controls, pre- and post-operative ccRCC patients, 24 were significantly different (P
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0111364
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID621623756
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1371/journal.pone.0111364
sourceidplos
recordidTN_plos10.1371/journal.pone.0111364
sourcesystemPC
pqid1621214636
galeid416975626
display
typearticle
titleIdentification of Potential Serum Proteomic Biomarkers for Clear Cell Renal Cell Carcinoma (Serum Biomarkers Detection for Clear Cell Renal Cell Carcinoma)
creatorYang, Juan ; Yang, Jin ; Gao, Yan ; Zhao, Lingyu ; Liu, Liying ; Qin, Yannan ; Wang, Xiaofei ; Song, Tusheng ; Huang, Chen
contributorHoheisel, Jörg D. (Editor)
ispartof2014, Vol.9(11), p.e111364
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0111364
subjectResearch Article ; Medicine And Health Sciences
descriptionTo investigate discriminating protein patterns and serum biomarkers between clear cell renal cell carcinoma (ccRCC) patients and healthy controls, as well as between paired pre- and post-operative ccRCC patients. ; We used magnetic bead-based separation followed by matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to identify patients with ccRCC. A total of 162 serum samples were analyzed in this study, among which there were 58 serum samples from ccRCC patients, 40 from additional paired pre- and post-operative ccRCC patients (n = 20), and 64 from healthy volunteers as healthy controls. ClinProTools software identified several distinct markers between ccRCC patients and healthy controls, as well as between pre- and post-operative patients. ; Patients with ccRCC could be identified with a mean sensitivity of 88.38% and a mean specificity of 91.67%. Of 67 m/z peaks that differed among the ccRCC, healthy controls, pre- and post-operative ccRCC patients, 24 were significantly different (P<0.05). Three candidate peaks, which were upregulated in ccRCC group and showed a tendency to return to healthy control values after surgery, were identified as peptide regions of RNA-binding protein 6 (RBP6), tubulin beta chain (TUBB), and zinc finger protein 3 (ZFP3) with the m/z values of 1466.98, 1618.22, and 5905.23, respectively. ; MB-MALDI-TOF-MS method could generate serum peptidome profiles of ccRCC, and provide a new approach to identify potential biomarkers for diagnosis as well as prognosis of this malignancy.
languageeng
source
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Yang, Juan
1Yang, Jin
2Gao, Yan
3Zhao, Lingyu
4Liu, Liying
5Qin, Yannan
6Wang, Xiaofei
7Song, Tusheng
8Huang, Chen
9Hoheisel, Jörg D. (Editor)
titleIdentification of Potential Serum Proteomic Biomarkers for Clear Cell Renal Cell Carcinoma (Serum Biomarkers Detection for Clear Cell Renal Cell Carcinoma)
descriptionTo investigate discriminating protein patterns and serum biomarkers between clear cell renal cell carcinoma (ccRCC) patients and healthy controls, as well as between paired pre- and post-operative ccRCC patients. ; We used magnetic bead-based separation followed by matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to identify patients with ccRCC. A total of 162 serum samples were analyzed in this study, among which there were 58 serum samples from ccRCC patients, 40 from additional paired pre- and post-operative ccRCC patients (n = 20), and 64 from healthy volunteers as healthy controls. ClinProTools software identified several distinct markers between ccRCC patients and healthy controls, as well as between pre- and post-operative patients. ; Patients with ccRCC could be identified with a mean sensitivity of 88.38% and a mean specificity of 91.67%. Of 67 m/z peaks that differed among the ccRCC, healthy controls, pre- and post-operative ccRCC patients, 24 were significantly different (P<0.05). Three candidate peaks, which were upregulated in ccRCC group and showed a tendency to return to healthy control values after surgery, were identified as peptide regions of RNA-binding protein 6 (RBP6), tubulin beta chain (TUBB), and zinc finger protein 3 (ZFP3) with the m/z values of 1466.98, 1618.22, and 5905.23, respectively. ; MB-MALDI-TOF-MS method could generate serum peptidome profiles of ccRCC, and provide a new approach to identify potential biomarkers for diagnosis as well as prognosis of this malignancy.
subject
0Research Article
1Medicine And Health Sciences
general
010.1371/journal.pone.0111364
1English
sourceidplos
recordidplos10.1371/journal.pone.0111364
issn
01932-6203
119326203
rsrctypearticle
creationdate2014
recordtypearticle
addtitleSerum Biomarkers Detection for Clear Cell Renal Cell Carcinoma
searchscopeplos
scopeplos
lsr30VSR-Enriched:[galeid, pqid]
sort
titleIdentification of Potential Serum Proteomic Biomarkers for Clear Cell Renal Cell Carcinoma (Serum Biomarkers Detection for Clear Cell Renal Cell Carcinoma)
authorYang, Juan ; Yang, Jin ; Gao, Yan ; Zhao, Lingyu ; Liu, Liying ; Qin, Yannan ; Wang, Xiaofei ; Song, Tusheng ; Huang, Chen
creationdate20141104
facets
frbrgroupid7615622832835716750
frbrtype5
languageeng
creationdate2014
topic
0Research Article
1Medicine And Health Sciences
collectionPLoS
prefilterarticles
rsrctypearticles
creatorcontrib
0Yang, Juan
1Yang, Jin
2Gao, Yan
3Zhao, Lingyu
4Liu, Liying
5Qin, Yannan
6Wang, Xiaofei
7Song, Tusheng
8Huang, Chen
9Hoheisel, Jörg D.
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Yang
1Gao
2Zhao
3Liu
4Qin
5Wang
6Song
7Huang
8Hoheisel
aufirst
0Juan
1Jin
2Yan
3Lingyu
4Liying
5Yannan
6Xiaofei
7Tusheng
8Chen
9Jörg D.
au
0Yang, Juan
1Yang, Jin
2Gao, Yan
3Zhao, Lingyu
4Liu, Liying
5Qin, Yannan
6Wang, Xiaofei
7Song, Tusheng
8Huang, Chen
addauHoheisel, Jörg D.
atitleIdentification of Potential Serum Proteomic Biomarkers for Clear Cell Renal Cell Carcinoma (Serum Biomarkers Detection for Clear Cell Renal Cell Carcinoma)
risdate20141104
volume9
issue11
spagee111364
pagese111364
eissn1932-6203
genrearticle
ristypeJOUR
abstractTo investigate discriminating protein patterns and serum biomarkers between clear cell renal cell carcinoma (ccRCC) patients and healthy controls, as well as between paired pre- and post-operative ccRCC patients. ; We used magnetic bead-based separation followed by matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to identify patients with ccRCC. A total of 162 serum samples were analyzed in this study, among which there were 58 serum samples from ccRCC patients, 40 from additional paired pre- and post-operative ccRCC patients (n = 20), and 64 from healthy volunteers as healthy controls. ClinProTools software identified several distinct markers between ccRCC patients and healthy controls, as well as between pre- and post-operative patients. ; Patients with ccRCC could be identified with a mean sensitivity of 88.38% and a mean specificity of 91.67%. Of 67 m/z peaks that differed among the ccRCC, healthy controls, pre- and post-operative ccRCC patients, 24 were significantly different (P<0.05). Three candidate peaks, which were upregulated in ccRCC group and showed a tendency to return to healthy control values after surgery, were identified as peptide regions of RNA-binding protein 6 (RBP6), tubulin beta chain (TUBB), and zinc finger protein 3 (ZFP3) with the m/z values of 1466.98, 1618.22, and 5905.23, respectively. ; MB-MALDI-TOF-MS method could generate serum peptidome profiles of ccRCC, and provide a new approach to identify potential biomarkers for diagnosis as well as prognosis of this malignancy.
copSan Francisco, USA
pubPublic Library of Science
doi10.1371/journal.pone.0111364
oafree_for_read
date2014-11-04