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Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification

How organ identity is determined is a fundamental question in developmental biology. In Drosophila , field-specific selector genes, such as eyeless ( ey ) for eyes and vestigial ( vg ) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function scree... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America 01 November 2005, Vol.102(44), p.15918
Main Author: Tomonori Katsuyama
Other Authors: Tomo Sugawara , Masanobu Tatsumi , Yoshiteru Oshima , Walter J. Gehring , Toshiro Aigaki , Shoichiro Kurata
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0027-8424 ; E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.0507945102
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recordid: pnas_s102_44_15918
title: Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification
format: Article
creator:
  • Tomonori Katsuyama
  • Tomo Sugawara
  • Masanobu Tatsumi
  • Yoshiteru Oshima
  • Walter J. Gehring
  • Toshiro Aigaki
  • Shoichiro Kurata
subjects:
  • Sciences (General)
ispartof: Proceedings of the National Academy of Sciences of the United States of America, 01 November 2005, Vol.102(44), p.15918
description: How organ identity is determined is a fundamental question in developmental biology. In Drosophila , field-specific selector genes, such as eyeless ( ey ) for eyes and vestigial ( vg ) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye ( wge ), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge -induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-( Antennapedia ) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent ( eya ), a control gene for eye development downstream of ey , in eye discs. In contrast to vg , however, overexpression of wge inhibited EY-mediated expression of eya . Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc . These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes. determination imaginal disc organ identity epigenetic regulation
language: eng
source:
identifier: ISSN: 0027-8424 ; E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.0507945102
fulltext: fulltext_linktorsrc
issn:
  • 0027-8424
  • 00278424
  • 1091-6490
  • 10916490
url: Link


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titleInvolvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification
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descriptionHow organ identity is determined is a fundamental question in developmental biology. In Drosophila , field-specific selector genes, such as eyeless ( ey ) for eyes and vestigial ( vg ) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye ( wge ), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge -induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-( Antennapedia ) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent ( eya ), a control gene for eye development downstream of ey , in eye discs. In contrast to vg , however, overexpression of wge inhibited EY-mediated expression of eya . Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc . These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes. determination imaginal disc organ identity epigenetic regulation
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titleInvolvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification
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How organ identity is determined is a fundamental question in developmental biology. In Drosophila , field-specific selector genes, such as eyeless ( ey ) for eyes and vestigial ( vg ) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye ( wge ), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge -induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-( Antennapedia ) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent ( eya ), a control gene for eye development downstream of ey , in eye discs. In contrast to vg , however, overexpression of wge inhibited EY-mediated expression of eya . Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc . These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes. determination imaginal disc organ identity epigenetic regulation

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How organ identity is determined is a fundamental question in developmental biology. In Drosophila , field-specific selector genes, such as eyeless ( ey ) for eyes and vestigial ( vg ) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye ( wge ), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge -induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-( Antennapedia ) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent ( eya ), a control gene for eye development downstream of ey , in eye discs. In contrast to vg , however, overexpression of wge inhibited EY-mediated expression of eya . Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc . These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes. determination imaginal disc organ identity epigenetic regulation

pubNational Acad Sciences
doi10.1073/pnas.0507945102
urlhttp://www.pnas.org/content/102/44/15918.abstract
lad01Proceedings of the National Academy of Sciences of the United States of America
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date2005-11-01