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Phosphatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate regulate phagolysosome biogenesis

Professional phagocytic cells ingest microbial intruders by engulfing them into phagosomes, which subsequently mature into microbicidal phagolysosomes. Phagosome maturation requires sequential fusion of the phagosome with early endosomes, late endosomes, and lysosomes. Although various phosphoinosit... Full description

Journal Title: Proceedings of the National Academy of Sciences 14 April 2015, Vol.112(15), p.4636
Main Author: Andreas Jeschke
Other Authors: Nicole Zehethofer , Buko Lindner , Jessica Krupp , Dominik Schwudke , Ina Haneburger , Marko Jovic , Jonathan M. Backer , Tamas Balla , Hubert Hilbi , Albert Haas
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0027-8424 ; E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1423456112
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recordid: pnas_s112_15_4636
title: Phosphatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate regulate phagolysosome biogenesis
format: Article
creator:
  • Andreas Jeschke
  • Nicole Zehethofer
  • Buko Lindner
  • Jessica Krupp
  • Dominik Schwudke
  • Ina Haneburger
  • Marko Jovic
  • Jonathan M. Backer
  • Tamas Balla
  • Hubert Hilbi
  • Albert Haas
subjects:
  • Sciences (General)
ispartof: Proceedings of the National Academy of Sciences, 14 April 2015, Vol.112(15), p.4636
description: Professional phagocytic cells ingest microbial intruders by engulfing them into phagosomes, which subsequently mature into microbicidal phagolysosomes. Phagosome maturation requires sequential fusion of the phagosome with early endosomes, late endosomes, and lysosomes. Although various phosphoinositides (PIPs) have been detected on phagosomes, it remained unclear which PIPs actually govern phagosome maturation. Here, we analyzed the involvement of PIPs in fusion of phagosomes with various endocytic compartments and identified phosphatidylinositol 4-phosphate [PI(4)P], phosphatidylinositol 3-phosphate [PI(3)P], and the lipid kinases that generate these PIPs, as mediators of phagosome-lysosome fusion. Phagosomeearly endosome fusion required PI(3)P, yet did not depend on PI(4)P. Thus, PI(3)P regulates phagosome maturation at early and late stages, whereas PI(4)P is selectively required late in the pathway. phosphoinositides | cell-free membrane fusion | phagosome | lysosome | Legionella pneumophila
language: eng
source:
identifier: ISSN: 0027-8424 ; E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1423456112
fulltext: fulltext_linktorsrc
issn:
  • 0027-8424
  • 00278424
  • 1091-6490
  • 10916490
url: Link


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titlePhosphatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate regulate phagolysosome biogenesis
creatorAndreas Jeschke ; Nicole Zehethofer ; Buko Lindner ; Jessica Krupp ; Dominik Schwudke ; Ina Haneburger ; Marko Jovic ; Jonathan M. Backer ; Tamas Balla ; Hubert Hilbi ; Albert Haas
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descriptionProfessional phagocytic cells ingest microbial intruders by engulfing them into phagosomes, which subsequently mature into microbicidal phagolysosomes. Phagosome maturation requires sequential fusion of the phagosome with early endosomes, late endosomes, and lysosomes. Although various phosphoinositides (PIPs) have been detected on phagosomes, it remained unclear which PIPs actually govern phagosome maturation. Here, we analyzed the involvement of PIPs in fusion of phagosomes with various endocytic compartments and identified phosphatidylinositol 4-phosphate [PI(4)P], phosphatidylinositol 3-phosphate [PI(3)P], and the lipid kinases that generate these PIPs, as mediators of phagosome-lysosome fusion. Phagosomeearly endosome fusion required PI(3)P, yet did not depend on PI(4)P. Thus, PI(3)P regulates phagosome maturation at early and late stages, whereas PI(4)P is selectively required late in the pathway. phosphoinositides | cell-free membrane fusion | phagosome | lysosome | Legionella pneumophila
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