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Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation.

Decoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilita... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America April 10, 2012, Vol.109(15), pp.5750-5755
Main Author: Chang, Yao-Fu
Other Authors: Lee-Chang, Jennifer S , Imam, J Saadi , Buddavarapu, Kalyan Chakravarthy , Subaran, Sarah S , Sinha-Hikim, Amiya P , Gorospe, Myriam , Rao, Manjeet K
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1117837109
Link: http://search.proquest.com/docview/1000405604/?pq-origsite=primo
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title: Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation.
format: Article
creator:
  • Chang, Yao-Fu
  • Lee-Chang, Jennifer S
  • Imam, J Saadi
  • Buddavarapu, Kalyan Chakravarthy
  • Subaran, Sarah S
  • Sinha-Hikim, Amiya P
  • Gorospe, Myriam
  • Rao, Manjeet K
subjects:
  • Actin-Related Protein 2-3 Complex–Metabolism
  • Animals–Genetics
  • Base Sequence–Metabolism
  • Cell Differentiation–Genetics
  • Chromatin–Genetics
  • Enzyme Activation–Metabolism
  • Gene Expression Regulation–Metabolism
  • Haploidy–Genetics
  • Hela Cells–Metabolism
  • Humans–Metabolism
  • Male–Metabolism
  • Meiosis–Metabolism
  • Mice–Pathology
  • Micrornas–Ultrastructure
  • Molecular Sequence Data–Metabolism
  • Protamines–Pathology
  • Protein Binding–Abnormalities
  • Protein Biosynthesis–Pathology
  • RNA, Messenger–Ultrastructure
  • Reproduction
ispartof: Proceedings of the National Academy of Sciences of the United States of America, April 10, 2012, Vol.109(15), pp.5750-5755
description: Decoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating the sequestration of germ cell mRNAs into translationally inert ribonucleoprotein particles until they are later translated. Our studies reveal that loss of microRNA-dependent regulation of Arpc5, which controls the distribution of germ cell mRNAs between translationally active and inactive pools, results in abnormal round spermatid differentiation and impaired fertility, interestingly, Arpc5 functions as a broadly acting translational suppressor, as it inhibits translation initiation by blocking 80S formation and facilitates the transport of mRNAs to chromatoid/P bodies. These findings identify a unique role for actin-associated proteins in translational regulation, and suggest that mRNA-specific and general translational control mechanisms work in tandem to regulate critical germ cell differentiation events and diverse somatic cell functions. spermiogenesis | messenger ribonucleoprotein | Arp2/3 | processing body doi/10.1073/pnas.1117837109
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1117837109
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titleInteraction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation.
creatorChang, Yao-Fu ; Lee-Chang, Jennifer S ; Imam, J Saadi ; Buddavarapu, Kalyan Chakravarthy ; Subaran, Sarah S ; Sinha-Hikim, Amiya P ; Gorospe, Myriam ; Rao, Manjeet K
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identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1117837109
subjectActin-Related Protein 2-3 Complex–Metabolism ; Animals–Genetics ; Base Sequence–Metabolism ; Cell Differentiation–Genetics ; Chromatin–Genetics ; Enzyme Activation–Metabolism ; Gene Expression Regulation–Metabolism ; Haploidy–Genetics ; Hela Cells–Metabolism ; Humans–Metabolism ; Male–Metabolism ; Meiosis–Metabolism ; Mice–Pathology ; Micrornas–Ultrastructure ; Molecular Sequence Data–Metabolism ; Protamines–Pathology ; Protein Binding–Abnormalities ; Protein Biosynthesis–Pathology ; RNA, Messenger–Ultrastructure ; Reproduction
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descriptionDecoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating the sequestration of germ cell mRNAs into translationally inert ribonucleoprotein particles until they are later translated. Our studies reveal that loss of microRNA-dependent regulation of Arpc5, which controls the distribution of germ cell mRNAs between translationally active and inactive pools, results in abnormal round spermatid differentiation and impaired fertility, interestingly, Arpc5 functions as a broadly acting translational suppressor, as it inhibits translation initiation by blocking 80S formation and facilitates the transport of mRNAs to chromatoid/P bodies. These findings identify a unique role for actin-associated proteins in translational regulation, and suggest that mRNA-specific and general translational control mechanisms work in tandem to regulate critical germ cell differentiation events and diverse somatic cell functions. spermiogenesis | messenger ribonucleoprotein | Arp2/3 | processing body doi/10.1073/pnas.1117837109
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titleInteraction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation.
authorChang, Yao-Fu ; Lee-Chang, Jennifer S ; Imam, J Saadi ; Buddavarapu, Kalyan Chakravarthy ; Subaran, Sarah S ; Sinha-Hikim, Amiya P ; Gorospe, Myriam ; Rao, Manjeet K
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