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Glutathione peroxidase from the white shrimp Litopenaeus vannamei: characterization and its regulation upon pH and Cd exposure

Issue Title: Special Issue: South China Sea Ecotoxicology Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. Knowledge of the complement of GPx in shrimp is essential to understanding regulation and detoxification mechan... Full description

Journal Title: Ecotoxicology Aug 2012, Vol.21(6), pp.1585-92
Main Author: Wang, Lei
Other Authors: Wu, Juan , Wang, Wei-Na , Cai, Dan-Xia , Liu, Yuan , Wang, An-Li
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 09639292 ; E-ISSN: 15733017 ; DOI: 10.1007/s10646-012-0942-z
Link: http://search.proquest.com/docview/1026735253/?pq-origsite=primo
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title: Glutathione peroxidase from the white shrimp Litopenaeus vannamei: characterization and its regulation upon pH and Cd exposure
format: Article
creator:
  • Wang, Lei
  • Wu, Juan
  • Wang, Wei-Na
  • Cai, Dan-Xia
  • Liu, Yuan
  • Wang, An-Li
subjects:
  • Animals–Toxicity
  • Cadmium–Genetics
  • Cloning, Molecular–Metabolism
  • Environmental Exposure–Genetics
  • Escherichia Coli–Metabolism
  • Escherichia Coli–Drug Effects
  • Gene Expression Profiling–Enzymology
  • Gene Expression Regulation, Enzymologic–Enzymology
  • Glutathione Peroxidase–Enzymology
  • Glutathione Peroxidase–Enzymology
  • Hydrogen-Ion Concentration–Enzymology
  • Penaeidae–Enzymology
  • Penaeidae–Enzymology
  • Real-Time Polymerase Chain Reaction–Enzymology
  • Sequence Analysis, DNA–Enzymology
  • Stress, Physiological–Enzymology
  • Up-Regulation–Enzymology
  • Cadmium
  • Glutathione Peroxidase
ispartof: Ecotoxicology, Aug 2012, Vol.21(6), pp.1585-92
description: Issue Title: Special Issue: South China Sea Ecotoxicology Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. Knowledge of the complement of GPx in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress. In this study, we expressed GPx from white shrimp Litopenaeus vannamei in Escherichia coli, and then characterized the purified recombinant enzyme with respect to the effects of pH, temperature on its catalytic activity. Quantitative real-time PCR and western blot analysis were carried out to investigate the expression patterns of GPx in shrimp hepatopancreas exposed to Cd stress. A statistically significant increase in expression of GPx mRNA and protein was observed in the Cd stress at 24 h. By contrast, western blot showed a significant up-regulation in GPx protein expression at 12 h exposed pH stress (5.6 and 9.3, respectively). These results suggest that L. vannamei...
language: eng
source:
identifier: ISSN: 09639292 ; E-ISSN: 15733017 ; DOI: 10.1007/s10646-012-0942-z
fulltext: fulltext
issn:
  • 09639292
  • 0963-9292
  • 15733017
  • 1573-3017
url: Link


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titleGlutathione peroxidase from the white shrimp Litopenaeus vannamei: characterization and its regulation upon pH and Cd exposure
creatorWang, Lei ; Wu, Juan ; Wang, Wei-Na ; Cai, Dan-Xia ; Liu, Yuan ; Wang, An-Li
ispartofEcotoxicology, Aug 2012, Vol.21(6), pp.1585-92
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subjectAnimals–Toxicity ; Cadmium–Genetics ; Cloning, Molecular–Metabolism ; Environmental Exposure–Genetics ; Escherichia Coli–Metabolism ; Escherichia Coli–Drug Effects ; Gene Expression Profiling–Enzymology ; Gene Expression Regulation, Enzymologic–Enzymology ; Glutathione Peroxidase–Enzymology ; Glutathione Peroxidase–Enzymology ; Hydrogen-Ion Concentration–Enzymology ; Penaeidae–Enzymology ; Penaeidae–Enzymology ; Real-Time Polymerase Chain Reaction–Enzymology ; Sequence Analysis, DNA–Enzymology ; Stress, Physiological–Enzymology ; Up-Regulation–Enzymology ; Cadmium ; Glutathione Peroxidase
descriptionIssue Title: Special Issue: South China Sea Ecotoxicology Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. Knowledge of the complement of GPx in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress. In this study, we expressed GPx from white shrimp Litopenaeus vannamei in Escherichia coli, and then characterized the purified recombinant enzyme with respect to the effects of pH, temperature on its catalytic activity. Quantitative real-time PCR and western blot analysis were carried out to investigate the expression patterns of GPx in shrimp hepatopancreas exposed to Cd stress. A statistically significant increase in expression of GPx mRNA and protein was observed in the Cd stress at 24 h. By contrast, western blot showed a significant up-regulation in GPx protein expression at 12 h exposed pH stress (5.6 and 9.3, respectively). These results suggest that L. vannamei...
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titleGlutathione peroxidase from the white shrimp Litopenaeus vannamei: characterization and its regulation upon pH and Cd exposure
descriptionIssue Title: Special Issue: South China Sea Ecotoxicology Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. Knowledge of the complement of GPx in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress. In this study, we expressed GPx from white shrimp Litopenaeus vannamei in Escherichia coli, and then characterized the purified recombinant enzyme with respect to the effects of pH, temperature on its catalytic activity. Quantitative real-time PCR and western blot analysis were carried out to investigate the expression patterns of GPx in shrimp hepatopancreas exposed to Cd stress. A statistically significant increase in expression of GPx mRNA and protein was observed in the Cd stress at 24 h. By contrast, western blot showed a significant up-regulation in GPx protein expression at 12 h exposed pH stress (5.6 and 9.3, respectively). These results suggest that L. vannamei...
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10Penaeidae–Enzymology
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12Sequence Analysis, DNA–Enzymology
13Stress, Physiological–Enzymology
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titleGlutathione peroxidase from the white shrimp Litopenaeus vannamei: characterization and its regulation upon pH and Cd exposure
authorWang, Lei ; Wu, Juan ; Wang, Wei-Na ; Cai, Dan-Xia ; Liu, Yuan ; Wang, An-Li
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6Gene Expression Profiling–Enzymology
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abstractIssue Title: Special Issue: South China Sea Ecotoxicology Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. Knowledge of the complement of GPx in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress. In this study, we expressed GPx from white shrimp Litopenaeus vannamei in Escherichia coli, and then characterized the purified recombinant enzyme with respect to the effects of pH, temperature on its catalytic activity. Quantitative real-time PCR and western blot analysis were carried out to investigate the expression patterns of GPx in shrimp hepatopancreas exposed to Cd stress. A statistically significant increase in expression of GPx mRNA and protein was observed in the Cd stress at 24 h. By contrast, western blot showed a significant up-regulation in GPx protein expression at 12 h exposed pH stress (5.6 and 9.3, respectively). These results suggest that L. vannamei...
copNew York
pubSpringer Nature B.V.
doi10.1007/s10646-012-0942-z
urlhttp://search.proquest.com/docview/1026735253/
date2012-08