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PI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.

To study the roles of phosphoinositides in the plasma membrane of mammalian cells, Hammond et al. (p. 727, published online 21 June; see the Perspective by Fairn and Grinstein) engineered phosphatase molecules that could be targeted to the membrane on demand, where they would alter the concentration... Full description

Journal Title: Science (New York N.Y.), August 10, 2012, Vol.337(6095), pp.727-730
Main Author: Hammond, Gerald R V
Other Authors: Fischer, Michael J , Anderson, Karen E , Holdich, Jon , Koteci, Ardita , Balla, Tamas , Irvine, Robin F
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1095-9203 ; DOI: 10.1126/science.1222483
Link: http://search.proquest.com/docview/1033155495/?pq-origsite=primo
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title: PI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.
format: Article
creator:
  • Hammond, Gerald R V
  • Fischer, Michael J
  • Anderson, Karen E
  • Holdich, Jon
  • Koteci, Ardita
  • Balla, Tamas
  • Irvine, Robin F
subjects:
  • Animals–Metabolism
  • Cos Cells–Metabolism
  • Cell Membrane–Metabolism
  • Cercopithecus Aethiops–Antagonists & Inhibitors
  • Endocytosis–Biosynthesis
  • Hek293 Cells–Metabolism
  • Humans–Metabolism
  • Membrane Proteins–Genetics
  • Peptide Fragments–Metabolism
  • Phosphatidylinositol 4,5-Diphosphate–Metabolism
  • Phosphatidylinositol Phosphates–Metabolism
  • Phosphoric Monoester Hydrolases–Genetics
  • Polymers–Metabolism
  • Receptor, Muscarinic M1–Antagonists & Inhibitors
  • Recombinant Fusion Proteins–Metabolism
  • Saccharomyces Cerevisiae Proteins–Metabolism
  • Signal Transduction–Metabolism
  • Static Electricity–Metabolism
  • Trpv Cation Channels–Metabolism
  • Membrane Proteins
  • Peptide Fragments
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositol Phosphates
  • Polymers
  • Receptor, Muscarinic M1
  • Recombinant Fusion Proteins
  • Saccharomyces Cerevisiae Proteins
  • Trpv Cation Channels
  • Trpv1 Protein, Human
  • Phosphatidylinositol 4-Phosphate
  • Polyanions
  • Sac1 Protein, S Cerevisiae
  • Phosphoric Monoester Hydrolases
  • Phosphoinositide 5-Phosphatase
ispartof: Science (New York, N.Y.), August 10, 2012, Vol.337(6095), pp.727-730
description: To study the roles of phosphoinositides in the plasma membrane of mammalian cells, Hammond et al. (p. 727, published online 21 June; see the Perspective by Fairn and Grinstein) engineered phosphatase molecules that could be targeted to the membrane on demand, where they would alter the concentrations of the phospholipids phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and phosphatidylinositol 4-phosphate (PI4P). PI4P was thought to provide a major source for the synthesis of PI(4,5)P2, but depletion of PI4P did not have much affect on synthesis of PI(4,5)P2. Instead, PI4P appears to help to establish the negative charge at the membrane and thus promote electrostatic interactions with positively charged amino acids in membrane-associated proteins and influencing function of ion channels. [PUBLICATION ] The quantitatively minor phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] fulfills many cellular functions in the plasma membrane (PM), whereas its synthetic precursor,...
language: eng
source:
identifier: E-ISSN: 1095-9203 ; DOI: 10.1126/science.1222483
fulltext: no_fulltext
issn:
  • 10959203
  • 1095-9203
url: Link


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titlePI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.
creatorHammond, Gerald R V ; Fischer, Michael J ; Anderson, Karen E ; Holdich, Jon ; Koteci, Ardita ; Balla, Tamas ; Irvine, Robin F
contributorHammond, Gerald R V (correspondence author) ; Hammond, Gerald R V (record owner)
ispartofScience (New York, N.Y.), August 10, 2012, Vol.337(6095), pp.727-730
identifierE-ISSN: 1095-9203 ; DOI: 10.1126/science.1222483
subjectAnimals–Metabolism ; Cos Cells–Metabolism ; Cell Membrane–Metabolism ; Cercopithecus Aethiops–Antagonists & Inhibitors ; Endocytosis–Biosynthesis ; Hek293 Cells–Metabolism ; Humans–Metabolism ; Membrane Proteins–Genetics ; Peptide Fragments–Metabolism ; Phosphatidylinositol 4,5-Diphosphate–Metabolism ; Phosphatidylinositol Phosphates–Metabolism ; Phosphoric Monoester Hydrolases–Genetics ; Polymers–Metabolism ; Receptor, Muscarinic M1–Antagonists & Inhibitors ; Recombinant Fusion Proteins–Metabolism ; Saccharomyces Cerevisiae Proteins–Metabolism ; Signal Transduction–Metabolism ; Static Electricity–Metabolism ; Trpv Cation Channels–Metabolism ; Membrane Proteins ; Peptide Fragments ; Phosphatidylinositol 4,5-Diphosphate ; Phosphatidylinositol Phosphates ; Polymers ; Receptor, Muscarinic M1 ; Recombinant Fusion Proteins ; Saccharomyces Cerevisiae Proteins ; Trpv Cation Channels ; Trpv1 Protein, Human ; Phosphatidylinositol 4-Phosphate ; Polyanions ; Sac1 Protein, S Cerevisiae ; Phosphoric Monoester Hydrolases ; Phosphoinositide 5-Phosphatase
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descriptionTo study the roles of phosphoinositides in the plasma membrane of mammalian cells, Hammond et al. (p. 727, published online 21 June; see the Perspective by Fairn and Grinstein) engineered phosphatase molecules that could be targeted to the membrane on demand, where they would alter the concentrations of the phospholipids phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] and phosphatidylinositol 4-phosphate (PI4P). PI4P was thought to provide a major source for the synthesis of PI(4,5)P2, but depletion of PI4P did not have much affect on synthesis of PI(4,5)P2. Instead, PI4P appears to help to establish the negative charge at the membrane and thus promote electrostatic interactions with positively charged amino acids in membrane-associated proteins and influencing function of ion channels. [PUBLICATION ] The quantitatively minor phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] fulfills many cellular functions in the plasma membrane (PM), whereas its synthetic precursor,...
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titlePI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.
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0Animals–Metabolism
1Cos Cells–Metabolism
2Cell Membrane–Metabolism
3Cercopithecus Aethiops–Antagonists & Inhibitors
4Endocytosis–Biosynthesis
5Hek293 Cells–Metabolism
6Humans–Metabolism
7Membrane Proteins–Genetics
8Peptide Fragments–Metabolism
9Phosphatidylinositol 4,5-Diphosphate–Metabolism
10Phosphatidylinositol Phosphates–Metabolism
11Phosphoric Monoester Hydrolases–Genetics
12Polymers–Metabolism
13Receptor, Muscarinic M1–Antagonists & Inhibitors
14Recombinant Fusion Proteins–Metabolism
15Saccharomyces Cerevisiae Proteins–Metabolism
16Signal Transduction–Metabolism
17Static Electricity–Metabolism
18Trpv Cation Channels–Metabolism
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20Peptide Fragments
21Phosphatidylinositol 4,5-Diphosphate
22Phosphatidylinositol Phosphates
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24Receptor, Muscarinic M1
25Recombinant Fusion Proteins
26Saccharomyces Cerevisiae Proteins
27Trpv Cation Channels
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titlePI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.
authorHammond, Gerald R V ; Fischer, Michael J ; Anderson, Karen E ; Holdich, Jon ; Koteci, Ardita ; Balla, Tamas ; Irvine, Robin F
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3Cercopithecus Aethiops–Antagonists & Inhibitors
4Endocytosis–Biosynthesis
5Hek293 Cells–Metabolism
6Humans–Metabolism
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8Peptide Fragments–Metabolism
9Phosphatidylinositol 4,5-Diphosphate–Metabolism
10Phosphatidylinositol Phosphates–Metabolism
11Phosphoric Monoester Hydrolases–Genetics
12Polymers–Metabolism
13Receptor, Muscarinic M1–Antagonists & Inhibitors
14Recombinant Fusion Proteins–Metabolism
15Saccharomyces Cerevisiae Proteins–Metabolism
16Signal Transduction–Metabolism
17Static Electricity–Metabolism
18Trpv Cation Channels–Metabolism
19Membrane Proteins
20Peptide Fragments
21Phosphatidylinositol 4,5-Diphosphate
22Phosphatidylinositol Phosphates
23Polymers
24Receptor, Muscarinic M1
25Recombinant Fusion Proteins
26Saccharomyces Cerevisiae Proteins
27Trpv Cation Channels
28Trpv1 Protein, Human
29Phosphatidylinositol 4-Phosphate
30Polyanions
31Sac1 Protein, S Cerevisiae
32Phosphoric Monoester Hydrolases
33Phosphoinositide 5-Phosphatase
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