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MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer.

Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metaboli... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America August 21, 2012, Vol.109(34), pp.13787-13792
Main Author: Chaika, Nina V
Other Authors: Gebregiworgis, Teklab , Lewallen, Michelle E , Purohit, Vinee , Radhakrishnan, Prakash , Liu, Xiang , Zhang, Bo , Mehla, Kamiya , Brown, Roger B , Caffrey, Thomas , Yu, Fang , Johnson, Keith R , Powers, Robert , Hollingsworth, Michael A , Singh, Pankaj K
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1203339109
Link: http://search.proquest.com/docview/1034802167/?pq-origsite=primo
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title: MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer.
format: Article
creator:
  • Chaika, Nina V
  • Gebregiworgis, Teklab
  • Lewallen, Michelle E
  • Purohit, Vinee
  • Radhakrishnan, Prakash
  • Liu, Xiang
  • Zhang, Bo
  • Mehla, Kamiya
  • Brown, Roger B
  • Caffrey, Thomas
  • Yu, Fang
  • Johnson, Keith R
  • Powers, Robert
  • Hollingsworth, Michael A
  • Singh, Pankaj K
subjects:
  • Animals–Metabolism
  • Female–Metabolism
  • Gene Expression Regulation, Neoplastic–Metabolism
  • Glucose–Metabolism
  • Glutamine–Chemistry
  • Glycolysis–Physiology
  • Humans–Metabolism
  • Hypoxia-Inducible Factor 1, Alpha Subunit–Metabolism
  • Ketoglutaric Acids–Metabolism
  • Mice–Metabolism
  • Mice, Nude–Metabolism
  • Models, Biological–Metabolism
  • Mucin-1–Metabolism
  • Pancreatic Neoplasms–Metabolism
  • Pentose Phosphate Pathway–Metabolism
  • Promoter Regions, Genetic–Metabolism
  • Signal Transduction–Metabolism
  • P300-Cbp Transcription Factors–Metabolism
  • Hypoxia-Inducible Factor 1, Alpha Subunit
  • Ketoglutaric Acids
  • Mucin-1
  • Glutamine
  • P300-Cbp Transcription Factors
  • Glucose
ispartof: Proceedings of the National Academy of Sciences of the United States of America, August 21, 2012, Vol.109(34), pp.13787-13792
description: Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate growth properties of cancer cells. MUC1 occupies the promoter elements of multiple genes directly involved in glucose metabolism and regulates their expression. Furthermore, MUC1 expression enhances glycolytic activity in pancreatic cancer cells. We also demonstrate that MUC1 expression enhances in vivo glucose uptake and expression of genes involved in glucose uptake and metabolism in orthotopic implantation models of pancreatic cancer. The MUC1 cytoplasmic tail is known to activate multiple signaling pathways through its interactions with several transcription factors/coregulators at the promoter elements of various genes. Our results indicate that MUC1 acts as a modulator of the hypoxic response in pancreatic cancer cells by regulating the expression/stability and activity of hypoxia-inducible factor-1[alpha] (HIF-1[alpha]). MUC1 physically interacts with HIF-1[alpha] and p300 and stabilizes the former at the protein level. By using a ChIP assay, we demonstrate that MUC1 facilitates recruitment of HIF-1[alpha] and p300 on glycolytic gene promoters in a hypoxia-dependent manner. Also, by metabolomic studies, we demonstrate that MUC1 regulates multiple metabolite intermediates in the glucose and amino acid metabolic pathways. Thus, our studies indicate that MUC1 acts as a master regulator of the metabolic program and facilitates metabolic alterations in the hypoxic environments that help tumor cells survive and proliferate under such conditions. cancer metabolism | glutamine accumulation | pentose phosphate pathway | 2-ketoglutarate www.pnas.org/cgi/doi/10.1073/pnas.1203339109
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1203339109
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titleMUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer.
creatorChaika, Nina V ; Gebregiworgis, Teklab ; Lewallen, Michelle E ; Purohit, Vinee ; Radhakrishnan, Prakash ; Liu, Xiang ; Zhang, Bo ; Mehla, Kamiya ; Brown, Roger B ; Caffrey, Thomas ; Yu, Fang ; Johnson, Keith R ; Powers, Robert ; Hollingsworth, Michael A ; Singh, Pankaj K
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identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1203339109
subjectAnimals–Metabolism ; Female–Metabolism ; Gene Expression Regulation, Neoplastic–Metabolism ; Glucose–Metabolism ; Glutamine–Chemistry ; Glycolysis–Physiology ; Humans–Metabolism ; Hypoxia-Inducible Factor 1, Alpha Subunit–Metabolism ; Ketoglutaric Acids–Metabolism ; Mice–Metabolism ; Mice, Nude–Metabolism ; Models, Biological–Metabolism ; Mucin-1–Metabolism ; Pancreatic Neoplasms–Metabolism ; Pentose Phosphate Pathway–Metabolism ; Promoter Regions, Genetic–Metabolism ; Signal Transduction–Metabolism ; P300-Cbp Transcription Factors–Metabolism ; Hypoxia-Inducible Factor 1, Alpha Subunit ; Ketoglutaric Acids ; Mucin-1 ; Glutamine ; P300-Cbp Transcription Factors ; Glucose
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descriptionAberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate growth properties of cancer cells. MUC1 occupies the promoter elements of multiple genes directly involved in glucose metabolism and regulates their expression. Furthermore, MUC1 expression enhances glycolytic activity in pancreatic cancer cells. We also demonstrate that MUC1 expression enhances in vivo glucose uptake and expression of genes involved in glucose uptake and metabolism in orthotopic implantation models of pancreatic cancer. The MUC1 cytoplasmic tail is known to activate multiple signaling pathways through its interactions with several transcription factors/coregulators at the promoter elements of various genes. Our results indicate that MUC1 acts as a modulator of the hypoxic response in pancreatic cancer cells by regulating the expression/stability and activity of hypoxia-inducible factor-1[alpha] (HIF-1[alpha]). MUC1 physically interacts with HIF-1[alpha] and p300 and stabilizes the former at the protein level. By using a ChIP assay, we demonstrate that MUC1 facilitates recruitment of HIF-1[alpha] and p300 on glycolytic gene promoters in a hypoxia-dependent manner. Also, by metabolomic studies, we demonstrate that MUC1 regulates multiple metabolite intermediates in the glucose and amino acid metabolic pathways. Thus, our studies indicate that MUC1 acts as a master regulator of the metabolic program and facilitates metabolic alterations in the hypoxic environments that help tumor cells survive and proliferate under such conditions. cancer metabolism | glutamine accumulation | pentose phosphate pathway | 2-ketoglutarate www.pnas.org/cgi/doi/10.1073/pnas.1203339109
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titleMUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer.
authorChaika, Nina V ; Gebregiworgis, Teklab ; Lewallen, Michelle E ; Purohit, Vinee ; Radhakrishnan, Prakash ; Liu, Xiang ; Zhang, Bo ; Mehla, Kamiya ; Brown, Roger B ; Caffrey, Thomas ; Yu, Fang ; Johnson, Keith R ; Powers, Robert ; Hollingsworth, Michael A ; Singh, Pankaj K
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