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Laserspray ionization imaging of multiply charged ions using a commercial vacuum MALDI ion source.

This is the first report of imaging mass spectrometry (MS) from multiply charged ions at vacuum. Laserspray ionization (LSI) was recently extended to applications at vacuum producing electrospray ionization-like multiply charged ions directly from surfaces using a commercial intermediate pressure ma... Full description

Journal Title: Analytical chemistry November 6, 2012, Vol.84(21), pp.9079-9084
Main Author: Inutan, Ellen D
Other Authors: Wager-Miller, James , Mackie, Ken , Trimpin, Sarah
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 10.1021/ac301665h
Link: http://search.proquest.com/docview/1141533065/?pq-origsite=primo
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recordid: proquest1141533065
title: Laserspray ionization imaging of multiply charged ions using a commercial vacuum MALDI ion source.
format: Article
creator:
  • Inutan, Ellen D
  • Wager-Miller, James
  • Mackie, Ken
  • Trimpin, Sarah
subjects:
  • Amino Acid Sequence–Metabolism
  • Animals–Methods
  • Brain–Chemistry
  • Lasers–Metabolism
  • Mice–Metabolism
  • Molecular Imaging–Metabolism
  • Molecular Sequence Data–Metabolism
  • Myelin Basic Protein–Metabolism
  • Pressure–Metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization–Metabolism
  • Vacuum–Metabolism
  • Myelin Basic Protein
ispartof: Analytical chemistry, November 6, 2012, Vol.84(21), pp.9079-9084
description: This is the first report of imaging mass spectrometry (MS) from multiply charged ions at vacuum. Laserspray ionization (LSI) was recently extended to applications at vacuum producing electrospray ionization-like multiply charged ions directly from surfaces using a commercial intermediate pressure matrix-assisted laser desorption/ionization ion mobility spectrometry (IMS) MS instrument. Here, we developed a strategy to image multiply charged peptide ions. This is achieved by the use of 2-nitrophloroglucinol as matrix for spray deposition onto the tissue section and implementation of "soft" acquisition conditions including lower laser power and ion accelerating voltages similar to electrospray ionization-like conditions. Sufficient ion abundance is generated by the vacuum LSI method to employ IMS separation in imaging multiply charged ions obtained on a commercial mass spectrometer ion source without physical instrument modifications using the laser in the commercially available reflection geometry alignment. IMS gas-phase separation reduces the complexity of the ion signal from the tissue, especially for multiply charged relative to abundant singly charged ions from tissue lipids. We show examples of LSI tissue imaging from charge state +2 of three endogenous peptides consisting of between 1 and 16 amino acid residues from the acetylated N-terminal end of myelin basic protein: mass-to-charge (m/z) 795.81 (+2) molecular weight (MW) 1589.6, m/z 831.35 (+2) MW 1660.7, and m/z 917.40 (+2) MW 1832.8.
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 10.1021/ac301665h
fulltext: fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleLaserspray ionization imaging of multiply charged ions using a commercial vacuum MALDI ion source.
creatorInutan, Ellen D ; Wager-Miller, James ; Mackie, Ken ; Trimpin, Sarah
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ispartofAnalytical chemistry, November 6, 2012, Vol.84(21), pp.9079-9084
identifierE-ISSN: 1520-6882 ; DOI: 10.1021/ac301665h
subjectAmino Acid Sequence–Metabolism ; Animals–Methods ; Brain–Chemistry ; Lasers–Metabolism ; Mice–Metabolism ; Molecular Imaging–Metabolism ; Molecular Sequence Data–Metabolism ; Myelin Basic Protein–Metabolism ; Pressure–Metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization–Metabolism ; Vacuum–Metabolism ; Myelin Basic Protein
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descriptionThis is the first report of imaging mass spectrometry (MS) from multiply charged ions at vacuum. Laserspray ionization (LSI) was recently extended to applications at vacuum producing electrospray ionization-like multiply charged ions directly from surfaces using a commercial intermediate pressure matrix-assisted laser desorption/ionization ion mobility spectrometry (IMS) MS instrument. Here, we developed a strategy to image multiply charged peptide ions. This is achieved by the use of 2-nitrophloroglucinol as matrix for spray deposition onto the tissue section and implementation of "soft" acquisition conditions including lower laser power and ion accelerating voltages similar to electrospray ionization-like conditions. Sufficient ion abundance is generated by the vacuum LSI method to employ IMS separation in imaging multiply charged ions obtained on a commercial mass spectrometer ion source without physical instrument modifications using the laser in the commercially available reflection geometry alignment. IMS gas-phase separation reduces the complexity of the ion signal from the tissue, especially for multiply charged relative to abundant singly charged ions from tissue lipids. We show examples of LSI tissue imaging from charge state +2 of three endogenous peptides consisting of between 1 and 16 amino acid residues from the acetylated N-terminal end of myelin basic protein: mass-to-charge (m/z) 795.81 (+2) molecular weight (MW) 1589.6, m/z 831.35 (+2) MW 1660.7, and m/z 917.40 (+2) MW 1832.8.
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