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CARBAPENEM RESISTANCE DUE TO BLA sub(OXA-48) AMONG ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN A UNIVERSITY HOSPITAL, TURKEY

Bacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla sub(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a universi... Full description

Journal Title: Southeast Asian Journal of Tropical Medicine & Public Health Sep 2012, Vol.43(5), pp.1178-1185
Main Author: Nazik, H
Other Authors: Ongen, B , Ilktac, M , Aydin, S , Kuvat, N , Sahin, A , Yemisen, M , Mete, B , Durmus, I , Balkan, I , Yildiz, Y , Ergul, H
Format: Electronic Article Electronic Article
Language: English
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ID: ISSN: 0125-1562
Link: http://search.proquest.com/docview/1171878173/?pq-origsite=primo
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title: CARBAPENEM RESISTANCE DUE TO BLA sub(OXA-48) AMONG ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN A UNIVERSITY HOSPITAL, TURKEY
format: Article
creator:
  • Nazik, H
  • Ongen, B
  • Ilktac, M
  • Aydin, S
  • Kuvat, N
  • Sahin, A
  • Yemisen, M
  • Mete, B
  • Durmus, I
  • Balkan, I
  • Yildiz, Y
  • Ergul, H
subjects:
  • Meropenem
  • Ertapenem
  • Polymerase Chain Reaction
  • Carbapenems
  • Antibiotics
  • Diffusion
  • Carbapenemase
  • Karyotypes
  • Imipenem
  • Public Health
  • Hospitals
  • Escherichia Coli
  • Klebsiella Pneumoniae
  • Antibiotics & Antimicrobials
ispartof: Southeast Asian Journal of Tropical Medicine & Public Health, Sep 2012, Vol.43(5), pp.1178-1185
description: Bacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla sub(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a university hospital, Turkey. Ninety-two ESBL-producing isolates (66 E. coli, 26 K. pneumoniae) were obtained in 2010. Antibiotic susceptibility tests were performed using the disc diffusion method and VITEK 2 system. Carbapenemase activity was screened using modified Hodge test. Beta-lactamase genes were detected by PCR and bla sub(OXA-48)-positive amplicons were sequenced. Genetic relatedness among K. pneumoniae isolates was investigated by pulsed-field gel-electrophoresis (PFGE). Carbapenemase activity was detected in 1 E. coli and 9 K. pneumoniae isolates and 8 of the K. pneumoniae plus the E. coli isolates were resistant to ertapenem. Three K. pneumoniae and 1 E. coli isolates were resistant to imipenem. All 10 isolates were susceptible to meropenem. bla sub(OXA-48) was present in all 10 isolates. Additionally, 9 isolates contained at least one beta-lactamase gene, including bla sub(SHV), bla sub(CTX-M) and bla sub(VEB) type. PFGE revealed different karyotypes among 9 K. pneumoniae isolates suggesting that the dissemination of bla sub(OXA-48) gene was not spread by a single K. pneumoniae clone. Thus OXA-48-producing isolates found in carbapenem-susceptible strains according to CLSI guidelines.
language: eng
source:
identifier: ISSN: 0125-1562
fulltext: fulltext
issn:
  • 01251562
  • 0125-1562
url: Link


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titleCARBAPENEM RESISTANCE DUE TO BLA sub(OXA-48) AMONG ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN A UNIVERSITY HOSPITAL, TURKEY
creatorNazik, H ; Ongen, B ; Ilktac, M ; Aydin, S ; Kuvat, N ; Sahin, A ; Yemisen, M ; Mete, B ; Durmus, I ; Balkan, I ; Yildiz, Y ; Ergul, H
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ispartofSoutheast Asian Journal of Tropical Medicine & Public Health, Sep 2012, Vol.43(5), pp.1178-1185
identifierISSN: 0125-1562
subjectMeropenem ; Ertapenem ; Polymerase Chain Reaction ; Carbapenems ; Antibiotics ; Diffusion ; Carbapenemase ; Karyotypes ; Imipenem ; Public Health ; Hospitals ; Escherichia Coli ; Klebsiella Pneumoniae ; Antibiotics & Antimicrobials
descriptionBacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla sub(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a university hospital, Turkey. Ninety-two ESBL-producing isolates (66 E. coli, 26 K. pneumoniae) were obtained in 2010. Antibiotic susceptibility tests were performed using the disc diffusion method and VITEK 2 system. Carbapenemase activity was screened using modified Hodge test. Beta-lactamase genes were detected by PCR and bla sub(OXA-48)-positive amplicons were sequenced. Genetic relatedness among K. pneumoniae isolates was investigated by pulsed-field gel-electrophoresis (PFGE). Carbapenemase activity was detected in 1 E. coli and 9 K. pneumoniae isolates and 8 of the K. pneumoniae plus the E. coli isolates were resistant to ertapenem. Three K. pneumoniae and 1 E. coli isolates were resistant to imipenem. All 10 isolates were susceptible to meropenem. bla sub(OXA-48) was present in all 10 isolates. Additionally, 9 isolates contained at least one beta-lactamase gene, including bla sub(SHV), bla sub(CTX-M) and bla sub(VEB) type. PFGE revealed different karyotypes among 9 K. pneumoniae isolates suggesting that the dissemination of bla sub(OXA-48) gene was not spread by a single K. pneumoniae clone. Thus OXA-48-producing isolates found in carbapenem-susceptible strains according to CLSI guidelines.
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titleCARBAPENEM RESISTANCE DUE TO BLA sub(OXA-48) AMONG ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN A UNIVERSITY HOSPITAL, TURKEY
descriptionBacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla sub(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a university hospital, Turkey. Ninety-two ESBL-producing isolates (66 E. coli, 26 K. pneumoniae) were obtained in 2010. Antibiotic susceptibility tests were performed using the disc diffusion method and VITEK 2 system. Carbapenemase activity was screened using modified Hodge test. Beta-lactamase genes were detected by PCR and bla sub(OXA-48)-positive amplicons were sequenced. Genetic relatedness among K. pneumoniae isolates was investigated by pulsed-field gel-electrophoresis (PFGE). Carbapenemase activity was detected in 1 E. coli and 9 K. pneumoniae isolates and 8 of the K. pneumoniae plus the E. coli isolates were resistant to ertapenem. Three K. pneumoniae and 1 E. coli isolates were resistant to imipenem. All 10 isolates were susceptible to meropenem. bla sub(OXA-48) was present in all 10 isolates. Additionally, 9 isolates contained at least one beta-lactamase gene, including bla sub(SHV), bla sub(CTX-M) and bla sub(VEB) type. PFGE revealed different karyotypes among 9 K. pneumoniae isolates suggesting that the dissemination of bla sub(OXA-48) gene was not spread by a single K. pneumoniae clone. Thus OXA-48-producing isolates found in carbapenem-susceptible strains according to CLSI guidelines.
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titleCARBAPENEM RESISTANCE DUE TO BLA sub(OXA-48) AMONG ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN A UNIVERSITY HOSPITAL, TURKEY
authorNazik, H ; Ongen, B ; Ilktac, M ; Aydin, S ; Kuvat, N ; Sahin, A ; Yemisen, M ; Mete, B ; Durmus, I ; Balkan, I ; Yildiz, Y ; Ergul, H
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atitleCARBAPENEM RESISTANCE DUE TO BLA sub(OXA-48) AMONG ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN A UNIVERSITY HOSPITAL, TURKEY
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abstractBacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla sub(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a university hospital, Turkey. Ninety-two ESBL-producing isolates (66 E. coli, 26 K. pneumoniae) were obtained in 2010. Antibiotic susceptibility tests were performed using the disc diffusion method and VITEK 2 system. Carbapenemase activity was screened using modified Hodge test. Beta-lactamase genes were detected by PCR and bla sub(OXA-48)-positive amplicons were sequenced. Genetic relatedness among K. pneumoniae isolates was investigated by pulsed-field gel-electrophoresis (PFGE). Carbapenemase activity was detected in 1 E. coli and 9 K. pneumoniae isolates and 8 of the K. pneumoniae plus the E. coli isolates were resistant to ertapenem. Three K. pneumoniae and 1 E. coli isolates were resistant to imipenem. All 10 isolates were susceptible to meropenem. bla sub(OXA-48) was present in all 10 isolates. Additionally, 9 isolates contained at least one beta-lactamase gene, including bla sub(SHV), bla sub(CTX-M) and bla sub(VEB) type. PFGE revealed different karyotypes among 9 K. pneumoniae isolates suggesting that the dissemination of bla sub(OXA-48) gene was not spread by a single K. pneumoniae clone. Thus OXA-48-producing isolates found in carbapenem-susceptible strains according to CLSI guidelines.
urlhttp://search.proquest.com/docview/1171878173/
date2012-09-01