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Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.

Invading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn’s disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract. We s... Full description

Journal Title: PloS one 2012, Vol.7(12), p.e51727
Main Author: Chargui, Abderrahman
Other Authors: Cesaro, Annabelle , Mimouna, Sanda , Fareh, Mohamed , Brest, Patrick , Naquet, Philippe , Darfeuille-Michaud, Arlette , Hébuterne, Xavier , Mograbi, Baharia , Vouret-Craviari, Valérie , Hofman, Paul
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0051727
Link: http://search.proquest.com/docview/1273708227/?pq-origsite=primo
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title: Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
format: Article
creator:
  • Chargui, Abderrahman
  • Cesaro, Annabelle
  • Mimouna, Sanda
  • Fareh, Mohamed
  • Brest, Patrick
  • Naquet, Philippe
  • Darfeuille-Michaud, Arlette
  • Hébuterne, Xavier
  • Mograbi, Baharia
  • Vouret-Craviari, Valérie
  • Hofman, Paul
subjects:
  • Autophagy–Immunology
  • Bacterial Adhesion–Immunology
  • Cell Death–Immunology
  • Cell Line–Immunology
  • Escherichia Coli–Metabolism
  • Humans–Immunology
  • Inflammation–Microbiology
  • Neutrophils–Immunology
  • Signal Transduction–Microbiology
  • Signal Transduction–Pathology
ispartof: PloS one, 2012, Vol.7(12), p.e51727
description: Invading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn’s disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract. We show here that pathogenic adherent-invasive Escherichia coli (AIEC) isolated from CD patients are able to adhere and invade neutrophils, which represent the first line of defense against bacteria. Of particular interest, AIEC infection of neutrophil-like PLB-985 cells blocked autophagy at the autolysosomal step, which allowed intracellular survival of bacteria and exacerbated interleukin-8 (IL-8) production. Interestingly, this block in autophagy correlated with the induction of autophagic cell death. Likewise, stimulation of autophagy by nutrient starvation or rapamycin treatment reduced intracellular AIEC survival and IL-8 production. Finally, treatment with an inhibitor of autophagy decreased cell death of AIEC-infected neutrophil-like PLB-985 cells. In conclusion, excessive autophagy in AIEC infection triggered cell death of neutrophils.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0051727
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleSubversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
creatorChargui, Abderrahman ; Cesaro, Annabelle ; Mimouna, Sanda ; Fareh, Mohamed ; Brest, Patrick ; Naquet, Philippe ; Darfeuille-Michaud, Arlette ; Hébuterne, Xavier ; Mograbi, Baharia ; Vouret-Craviari, Valérie ; Hofman, Paul
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0051727
subjectAutophagy–Immunology ; Bacterial Adhesion–Immunology ; Cell Death–Immunology ; Cell Line–Immunology ; Escherichia Coli–Metabolism ; Humans–Immunology ; Inflammation–Microbiology ; Neutrophils–Immunology ; Signal Transduction–Microbiology ; Signal Transduction–Pathology
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descriptionInvading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn’s disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract. We show here that pathogenic adherent-invasive Escherichia coli (AIEC) isolated from CD patients are able to adhere and invade neutrophils, which represent the first line of defense against bacteria. Of particular interest, AIEC infection of neutrophil-like PLB-985 cells blocked autophagy at the autolysosomal step, which allowed intracellular survival of bacteria and exacerbated interleukin-8 (IL-8) production. Interestingly, this block in autophagy correlated with the induction of autophagic cell death. Likewise, stimulation of autophagy by nutrient starvation or rapamycin treatment reduced intracellular AIEC survival and IL-8 production. Finally, treatment with an inhibitor of autophagy decreased cell death of AIEC-infected neutrophil-like PLB-985 cells. In conclusion, excessive autophagy in AIEC infection triggered cell death of neutrophils.
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titleSubversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death.
authorChargui, Abderrahman ; Cesaro, Annabelle ; Mimouna, Sanda ; Fareh, Mohamed ; Brest, Patrick ; Naquet, Philippe ; Darfeuille-Michaud, Arlette ; Hébuterne, Xavier ; Mograbi, Baharia ; Vouret-Craviari, Valérie ; Hofman, Paul
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