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Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

Background Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic... Full description

Journal Title: PLoS One Jun 2012, Vol.7(6), p.e38875
Main Author: Bai, Yang
Other Authors: Yang, Weiwei , Hui-Xia, Yang , Liao, Qinping , Ye, Gang , Fu, Guodong , Ji, Lei , Xu, Peng , Wang, Hao , Yu-Xia, Li , Peng, Chun , Yan-Ling, Wang
Format: Electronic Article Electronic Article
Language: English
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ID: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0038875
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title: Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression
format: Article
creator:
  • Bai, Yang
  • Yang, Weiwei
  • Hui-Xia, Yang
  • Liao, Qinping
  • Ye, Gang
  • Fu, Guodong
  • Ji, Lei
  • Xu, Peng
  • Wang, Hao
  • Yu-Xia, Li
  • Peng, Chun
  • Yan-Ling, Wang
subjects:
  • Beijing China
  • China
  • Laboratories
  • Pregnancy
  • Placenta
  • Proteinuria
  • Zoology
  • Pregnancy
  • Pregnancy
  • Hypertension
  • Micrornas
  • Pregnancy
  • Epigenetics
  • Genes
  • Cell Cycle
  • Angiogenesis
  • Breast Cancer
  • Pre-Eclampsia
  • Signal Transduction
  • Placenta
ispartof: PLoS One, Jun 2012, Vol.7(6), p.e38875
description: Background Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown. Methodology/Principal Findings Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195...
language: eng
source:
identifier: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0038875
fulltext: fulltext_linktorsrc
issn:
  • 19326203
  • 1932-6203
url: Link


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titleDownregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression
creatorBai, Yang ; Yang, Weiwei ; Hui-Xia, Yang ; Liao, Qinping ; Ye, Gang ; Fu, Guodong ; Ji, Lei ; Xu, Peng ; Wang, Hao ; Yu-Xia, Li ; Peng, Chun ; Yan-Ling, Wang
ispartofPLoS One, Jun 2012, Vol.7(6), p.e38875
identifierE-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0038875
subjectBeijing China ; China ; Laboratories ; Pregnancy ; Placenta ; Proteinuria ; Zoology ; Pregnancy ; Pregnancy ; Hypertension ; Micrornas ; Pregnancy ; Epigenetics ; Genes ; Cell Cycle ; Angiogenesis ; Breast Cancer ; Pre-Eclampsia ; Signal Transduction ; Placenta
descriptionBackground Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown. Methodology/Principal Findings Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195...
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titleDownregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression
descriptionBackground Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown. Methodology/Principal Findings Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195...
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titleDownregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression
authorBai, Yang ; Yang, Weiwei ; Hui-Xia, Yang ; Liao, Qinping ; Ye, Gang ; Fu, Guodong ; Ji, Lei ; Xu, Peng ; Wang, Hao ; Yu-Xia, Li ; Peng, Chun ; Yan-Ling, Wang
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8Micrornas
9Epigenetics
10Genes
11Cell Cycle
12Angiogenesis
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15Signal Transduction
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atitleDownregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression
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abstractBackground Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown. Methodology/Principal Findings Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195...
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