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Preparation and characterization of liposome-encapsulated plasmid DNA for gene delivery.

The success of common nonviral gene delivery vehicles, lipoplexes and polyplexes, is limited by the toxicity and instability of these charged molecules. Stealth liposomes could provide a stable, safe alternative to cationic DNA complexes for effective gene delivery. DNA encapsulations in three steal... Full description

Journal Title: Langmuir : the ACS journal of surfaces and colloids July 23, 2013, Vol.29(29), pp.9208-9215
Main Author: Levine, Rachel M
Other Authors: Pearce, Timothy R , Adil, Maroof , Kokkoli, Efrosini
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: E-ISSN: 1520-5827 ; DOI: 1520-5827 ; DOI: 10.1021/la400859e
Link: http://search.proquest.com/docview/1412161362/?pq-origsite=primo
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recordid: proquest1412161362
title: Preparation and characterization of liposome-encapsulated plasmid DNA for gene delivery.
format: Article
creator:
  • Levine, Rachel M
  • Pearce, Timothy R
  • Adil, Maroof
  • Kokkoli, Efrosini
subjects:
  • Capsules–Administration & Dosage
  • DNA–Genetics
  • Gene Transfer Techniques–Metabolism
  • Genetic Therapy–Genetics
  • Liposomes–Genetics
  • Models, Molecular–Genetics
  • Molecular Conformation–Genetics
  • Plasmids–Genetics
  • Capsules
  • Liposomes
  • DNA
ispartof: Langmuir : the ACS journal of surfaces and colloids, July 23, 2013, Vol.29(29), pp.9208-9215
description: The success of common nonviral gene delivery vehicles, lipoplexes and polyplexes, is limited by the toxicity and instability of these charged molecules. Stealth liposomes could provide a stable, safe alternative to cationic DNA complexes for effective gene delivery. DNA encapsulations in three stealth liposomal formulations prepared by thin film, reverse phase evaporation, and asymmetric liposome formation were compared, and the thin film method was found to produce the highest yields of encapsulated DNA. A DNA quantification method appropriate for DNA encapsulated within liposomes was also developed and verified for accuracy. The effect of initial lipid and DNA concentrations on the encapsulation yield and fraction of DNA-filled liposomes was evaluated. Higher encapsulation yields were achieved by higher lipid contents, while a higher fraction of DNA-filled liposomes was produced by either lower lipid content or higher DNA concentration. Control of these parameters allows for the design of gene delivery nanoparticles with high DNA encapsulation yields or higher fraction of DNA-filled liposomes.
language: eng
source:
identifier: E-ISSN: 1520-5827 ; DOI: 1520-5827 ; DOI: 10.1021/la400859e
fulltext: no_fulltext
issn:
  • 15205827
  • 1520-5827
url: Link


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titlePreparation and characterization of liposome-encapsulated plasmid DNA for gene delivery.
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descriptionThe success of common nonviral gene delivery vehicles, lipoplexes and polyplexes, is limited by the toxicity and instability of these charged molecules. Stealth liposomes could provide a stable, safe alternative to cationic DNA complexes for effective gene delivery. DNA encapsulations in three stealth liposomal formulations prepared by thin film, reverse phase evaporation, and asymmetric liposome formation were compared, and the thin film method was found to produce the highest yields of encapsulated DNA. A DNA quantification method appropriate for DNA encapsulated within liposomes was also developed and verified for accuracy. The effect of initial lipid and DNA concentrations on the encapsulation yield and fraction of DNA-filled liposomes was evaluated. Higher encapsulation yields were achieved by higher lipid contents, while a higher fraction of DNA-filled liposomes was produced by either lower lipid content or higher DNA concentration. Control of these parameters allows for the design of gene delivery nanoparticles with high DNA encapsulation yields or higher fraction of DNA-filled liposomes.
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