schliessen

Filtern

 

Bibliotheken

NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers.

The importance of dynamics to biomolecular function is becoming increasingly clear. A description of the structure-function relationship must, therefore, include the role of motion, requiring a shift in paradigm from focus on a single static 3D picture to one where a given biomolecule is considered... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America August 6, 2013, Vol.110(32), pp.12867-12874
Main Author: Sekhar, Ashok
Other Authors: Kay, Lewis E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1305688110
Link: http://search.proquest.com/docview/1418646087/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest1418646087
title: NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers.
format: Article
creator:
  • Sekhar, Ashok
  • Kay, Lewis E
subjects:
  • Calcium–Chemistry
  • Calmodulin–Metabolism
  • Models, Molecular–Chemistry
  • Nuclear Magnetic Resonance, Biomolecular–Metabolism
  • Protein Binding–Methods
  • Protein Conformation–Chemistry
  • Protein Folding–Metabolism
  • Protein Structure, Tertiary–Metabolism
  • Proto-Oncogene Proteins C-Fyn–Metabolism
  • Reproducibility of Results–Metabolism
  • Src Homology Domains–Metabolism
  • Calmodulin
  • Proto-Oncogene Proteins C-Fyn
  • Calcium
  • Energy Landscape
  • Invisible States
  • Structure–Function Paradigm
  • Transiently and Sparsely Populated Biomolecular States
ispartof: Proceedings of the National Academy of Sciences of the United States of America, August 6, 2013, Vol.110(32), pp.12867-12874
description: The importance of dynamics to biomolecular function is becoming increasingly clear. A description of the structure-function relationship must, therefore, include the role of motion, requiring a shift in paradigm from focus on a single static 3D picture to one where a given biomolecule is considered in terms of an ensemble of interconverting conformers, each with potentially diverse activities. In this Perspective, we describe how recent developments in solution NMR spectroscopy facilitate atomic resolution studies of sparsely populated, transiently formed biomolecular conformations that exchange with the native state. Examples of how this methodology is applied to protein folding and misfolding, ligand binding, and molecular recognition are provided as a means of illustrating both the power of the new techniques and the significant roles that conformationally excited protein states play in biology. energy landscape | transiently and sparsely populated biomolecular states | invisible states | structure-function paradigm www.pnas.org/cgi/doi/ 10.1073/pnas.1305688110
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1305688110
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


@attributes
ID47615129
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1418646087
sourceidproquest
recordidTN_proquest1418646087
sourcesystemPC
pqid1418646087
galeid342176652
display
typearticle
titleNMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers.
creatorSekhar, Ashok ; Kay, Lewis E
contributorSekhar, Ashok (correspondence author) ; Sekhar, Ashok (record owner)
ispartofProceedings of the National Academy of Sciences of the United States of America, August 6, 2013, Vol.110(32), pp.12867-12874
identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1305688110
subjectCalcium–Chemistry ; Calmodulin–Metabolism ; Models, Molecular–Chemistry ; Nuclear Magnetic Resonance, Biomolecular–Metabolism ; Protein Binding–Methods ; Protein Conformation–Chemistry ; Protein Folding–Metabolism ; Protein Structure, Tertiary–Metabolism ; Proto-Oncogene Proteins C-Fyn–Metabolism ; Reproducibility of Results–Metabolism ; Src Homology Domains–Metabolism ; Calmodulin ; Proto-Oncogene Proteins C-Fyn ; Calcium ; Energy Landscape ; Invisible States ; Structure–Function Paradigm ; Transiently and Sparsely Populated Biomolecular States
languageeng
source
descriptionThe importance of dynamics to biomolecular function is becoming increasingly clear. A description of the structure-function relationship must, therefore, include the role of motion, requiring a shift in paradigm from focus on a single static 3D picture to one where a given biomolecule is considered in terms of an ensemble of interconverting conformers, each with potentially diverse activities. In this Perspective, we describe how recent developments in solution NMR spectroscopy facilitate atomic resolution studies of sparsely populated, transiently formed biomolecular conformations that exchange with the native state. Examples of how this methodology is applied to protein folding and misfolding, ligand binding, and molecular recognition are provided as a means of illustrating both the power of the new techniques and the significant roles that conformationally excited protein states play in biology. energy landscape | transiently and sparsely populated biomolecular states | invisible states | structure-function paradigm www.pnas.org/cgi/doi/ 10.1073/pnas.1305688110
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/1418646087/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Sekhar, Ashok
1Kay, Lewis E
titleNMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers.
subject
0Calcium–Chemistry
1Calmodulin–Metabolism
2Models, Molecular–Chemistry
3Nuclear Magnetic Resonance, Biomolecular–Metabolism
4Protein Binding–Methods
5Protein Conformation–Chemistry
6Protein Folding–Metabolism
7Protein Structure, Tertiary–Metabolism
8Proto-Oncogene Proteins C-Fyn–Metabolism
9Reproducibility...
10energy landscape
11invisible states
12structure–function paradigm
13transiently and sparsely populated biomolecular states
general
0English
110.1073/pnas.1305688110
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest1418646087
issn
010916490
11091-6490
rsrctypearticle
creationdate2013
addtitleProceedings of the National Academy of Sciences of the United States of America
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorSekhar, Ashok
startdate20130806
enddate20130806
citationpf 12867 pt 12874 vol 110 issue 32
lsr30VSR-Enriched:[galeid, pqid, issn, description]
sort
titleNMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers.
authorSekhar, Ashok ; Kay, Lewis E
creationdate20130806
lso0120130806
facets
frbrgroupid9109759302590455204
frbrtype5
newrecords20181218
languageeng
creationdate2013
topic
0Calcium–Chemistry
1Calmodulin–Metabolism
2Models, Molecular–Chemistry
3Nuclear Magnetic Resonance, Biomolecular–Metabolism
4Protein Binding–Methods
5Protein Conformation–Chemistry
6Protein Folding–Metabolism
7Protein Structure, Tertiary–Metabolism
8Proto-Oncogene Proteins C-Fyn–Metabolism
9Reproducibility...
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Sekhar, Ashok
1Kay, Lewis E
jtitleProceedings of the National Academy of Sciences of the United States of America
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Sekhar
1Kay
aufirst
0Ashok
1Lewis E
au
0Sekhar, Ashok
1Kay, Lewis E
addauSekhar, Ashok
atitleNMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers.
jtitleProceedings of the National Academy of Sciences of the United States of America
risdate20130806
volume110
issue32
spage12867
epage12874
pages12867-12874
eissn1091-6490
formatjournal
genrearticle
ristypeJOUR
doi10.1073/pnas.1305688110
urlhttp://search.proquest.com/docview/1418646087/
issn00278424
date2013-08-06