schliessen

Filtern

 

Bibliotheken

Fate of the Donor Blastodermal Cells Derived from the Central Disc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos

  In order to trace the donor blastodermal cell movement in the recipient embryos, stage X blastodermal cells derived from the central disc (CD), marginal zone (MZ) and area opaca (AO) were transfected in vitro by lipofection using green fluorescent protein (GFP) gene as a marker and transferred the... Full description

Journal Title: The Journal of Poultry Science 2001, Vol.38(1), p.58
Main Author: Naito, Mitsuru
Other Authors: Sano, Akiko , Matsubara, Yuko , Harumi, Takashi , Tagami, Takahiro , Sakurai, Michiharu , Kuwana, Takashi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1439290196/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest1439290196
title: Fate of the Donor Blastodermal Cells Derived from the Central Disc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos
format: Article
creator:
  • Naito, Mitsuru
  • Sano, Akiko
  • Matsubara, Yuko
  • Harumi, Takashi
  • Tagami, Takahiro
  • Sakurai, Michiharu
  • Kuwana, Takashi
subjects:
  • Chicken
  • Blastodermal Cell
  • Gfp Gene
  • Gene Expression
  • Lipofection
ispartof: The Journal of Poultry Science, 2001, Vol.38(1), p.58
description:   In order to trace the donor blastodermal cell movement in the recipient embryos, stage X blastodermal cells derived from the central disc (CD), marginal zone (MZ) and area opaca (AO) were transfected in vitro by lipofection using green fluorescent protein (GFP) gene as a marker and transferred them into recipient blastoderms. Expression of the GFP gene in the whole blastoderm was observed in all the embryos manipulated at day 1 of incubation. After that, the GFP gene expression decreased with the incubation period and at day 3 of incubation the GFP gene expression was detected only in the extra-embryonic membranes. The frequency of the GFP gene expression at day 3 of incubation was lower when the donor blastodermal cells were derived from the CD compared with the MZ or AO, probably due to the active cell proliferation in the recipient embryos. The GFP gene is very useful as a marker to trace cell movement for short-term, but stable incorporation into the donor cell chromosomes is necessary for long-term tracing.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


@attributes
ID943264792
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1439290196
sourceidproquest
recordidTN_proquest1439290196
sourcesystemOther
pqid1439290196
display
typearticle
titleFate of the Donor Blastodermal Cells Derived from the Central Disc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos
creatorNaito, Mitsuru ; Sano, Akiko ; Matsubara, Yuko ; Harumi, Takashi ; Tagami, Takahiro ; Sakurai, Michiharu ; Kuwana, Takashi
ispartofThe Journal of Poultry Science, 2001, Vol.38(1), p.58
identifierISSN: 13467395 ; E-ISSN: 13490486
description  In order to trace the donor blastodermal cell movement in the recipient embryos, stage X blastodermal cells derived from the central disc (CD), marginal zone (MZ) and area opaca (AO) were transfected in vitro by lipofection using green fluorescent protein (GFP) gene as a marker and transferred them into recipient blastoderms. Expression of the GFP gene in the whole blastoderm was observed in all the embryos manipulated at day 1 of incubation. After that, the GFP gene expression decreased with the incubation period and at day 3 of incubation the GFP gene expression was detected only in the extra-embryonic membranes. The frequency of the GFP gene expression at day 3 of incubation was lower when the donor blastodermal cells were derived from the CD compared with the MZ or AO, probably due to the active cell proliferation in the recipient embryos. The GFP gene is very useful as a marker to trace cell movement for short-term, but stable incorporation into the donor cell chromosomes is necessary for long-term tracing.
languageeng
source
subjectChicken ; Blastodermal Cell ; Gfp Gene ; Gene Expression ; Lipofection;
version4
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/1439290196/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Naito, Mitsuru
1Sano, Akiko
2Matsubara, Yuko
3Harumi, Takashi
4Tagami, Takahiro
5Sakurai, Michiharu
6Kuwana, Takashi
titleFate of the Donor Blastodermal Cells Derived from the Central Disc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos
description  In order to trace the donor blastodermal cell movement in the recipient embryos, stage X blastodermal cells derived from the central disc (CD), marginal zone (MZ) and area opaca (AO) were transfected in vitro by lipofection using green fluorescent protein (GFP) gene as a marker and transferred them into recipient blastoderms. Expression of the GFP gene in the whole blastoderm was observed in all the embryos manipulated at day 1 of incubation. After that, the GFP gene expression decreased with the incubation period and at day 3 of incubation the GFP gene expression was detected only in the extra-embryonic membranes. The frequency of the GFP gene expression at day 3 of incubation was lower when the donor blastodermal cells were derived from the CD compared with the MZ or AO, probably due to the active cell proliferation in the recipient embryos. The GFP gene is very useful as a marker to trace cell movement for short-term, but stable incorporation into the donor cell chromosomes is necessary for long-term tracing.
general
0English
1Japan Science and Technology Agency
2ProQuest Agriculture Journals
3ProQuest Agricultural Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Agricultural & Environmental Science Database
7Natural Science Collection
8SciTech Premium Collection
sourceidproquest
recordidproquest1439290196
issn
013467395
11346-7395
213490486
31349-0486
rsrctypearticle
creationdate2001
addtitleThe Journal of Poultry Science
searchscope
01007160
11007945
21008045
31008046
41008886
51009043
610000035
710000050
810000118
910000120
1010000234
1110000253
1210000260
13proquest
scope
01007160
11007945
21008045
31008046
41008886
51009043
610000035
710000050
810000118
910000120
1010000234
1110000253
1210000260
13proquest
lsr43
01007160false
11007945false
21008045false
31008046false
41008886false
51009043false
610000035false
710000050false
810000118false
910000120false
1010000234false
1110000253false
1210000260false
startdate20010101
enddate20010101
citationpf 58 vol 38 issue 1
lsr30VSR-Enriched:[pqid, doi, pages, date, subject]
sort
titleFate of the Donor Blastodermal Cells Derived from the Central Disc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos
authorNaito, Mitsuru ; Sano, Akiko ; Matsubara, Yuko ; Harumi, Takashi ; Tagami, Takahiro ; Sakurai, Michiharu ; Kuwana, Takashi
creationdate20010101
lso0120010101
facets
frbrgroupid3703349134629914253
frbrtype5
languageeng
creationdate2001
collection
0ProQuest Agriculture Journals
1ProQuest Agricultural Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Agricultural & Environmental Science Database
5Natural Science Collection
6SciTech Premium Collection
prefilterarticles
rsrctypearticles
creatorcontrib
0Naito, Mitsuru
1Sano, Akiko
2Matsubara, Yuko
3Harumi, Takashi
4Tagami, Takahiro
5Sakurai, Michiharu
6Kuwana, Takashi
jtitleJournal of Poultry Science
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Naito
1Sano
2Matsubara
3Harumi
4Tagami
5Sakurai
6Kuwana
aufirst
0Mitsuru
1Akiko
2Yuko
3Takashi
4Takahiro
5Michiharu
auinit1
0M.
1A.
2Y.
3T.
au
0Naito, Mitsuru
1Sano, Akiko
2Matsubara, Yuko
3Harumi, Takashi
4Tagami, Takahiro
5Sakurai, Michiharu
6Kuwana, Takashi
atitleFate of the Donor Blastodermal Cells Derived from the Central Disc, Marginal Zone and Area Opaca Transferred into the Recipient Embryos
jtitleThe Journal of Poultry Science
date2001
risdate20010101
volume38
issue1
spage58
issn13467395
eissn13490486
formatjournal
genrearticle
ristypeJOUR
abstract  In order to trace the donor blastodermal cell movement in the recipient embryos, stage X blastodermal cells derived from the central disc (CD), marginal zone (MZ) and area opaca (AO) were transfected in vitro by lipofection using green fluorescent protein (GFP) gene as a marker and transferred them into recipient blastoderms. Expression of the GFP gene in the whole blastoderm was observed in all the embryos manipulated at day 1 of incubation. After that, the GFP gene expression decreased with the incubation period and at day 3 of incubation the GFP gene expression was detected only in the extra-embryonic membranes. The frequency of the GFP gene expression at day 3 of incubation was lower when the donor blastodermal cells were derived from the CD compared with the MZ or AO, probably due to the active cell proliferation in the recipient embryos. The GFP gene is very useful as a marker to trace cell movement for short-term, but stable incorporation into the donor cell chromosomes is necessary for long-term tracing.
copIbaraki
pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1439290196/
doi10.2141/jpsa.38.58
pages58-61