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A Simple Method for Sexing Chicken Embryos at Stage X

  A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the... Full description

Journal Title: The Journal of Poultry Science 2003, Vol.40(3), p.226
Main Author: Naito, Mitsuru
Other Authors: Sano, Akiko , Kawashima, Takaharu , Nakamichi, Hiroyuki , Harumi, Takashi , Matsubara, Yuko , Kuwana, Takashi
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
Pcr
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1439290676/?pq-origsite=primo
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recordid: proquest1439290676
title: A Simple Method for Sexing Chicken Embryos at Stage X
format: Article
creator:
  • Naito, Mitsuru
  • Sano, Akiko
  • Kawashima, Takaharu
  • Nakamichi, Hiroyuki
  • Harumi, Takashi
  • Matsubara, Yuko
  • Kuwana, Takashi
subjects:
  • Chick Embryos
  • DNA
  • Methodology
  • Polymerase Chain Reaction
  • Poultry
  • Sex Diagnosis
  • Chickens
  • Deoxyribonucleic Acid
  • Domesticated Birds
  • Methods
  • Pcr
  • Birds
  • Fowls
  • Gallus Gallus
  • Gallus
  • Phasianidae
  • Galliformes
  • Vertebrates
  • Chordata
  • Animals
  • Eukaryotes
ispartof: The Journal of Poultry Science, 2003, Vol.40(3), p.226
description:   A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the extraction method (DNA extraction kit) as a control. PCR analysis was carried out using the prepared DNA, and the sex of embryos was clearly identified by both the boiling and extraction method. This simple and rapid method of embryo sexing using blastodermal cells isolated from the area opaca enables us to identify the sex of a large number of chicken embryos in a limited time.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


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titleA Simple Method for Sexing Chicken Embryos at Stage X
creatorNaito, Mitsuru ; Sano, Akiko ; Kawashima, Takaharu ; Nakamichi, Hiroyuki ; Harumi, Takashi ; Matsubara, Yuko ; Kuwana, Takashi
ispartofThe Journal of Poultry Science, 2003, Vol.40(3), p.226
identifierISSN: 13467395 ; E-ISSN: 13490486
description  A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the extraction method (DNA extraction kit) as a control. PCR analysis was carried out using the prepared DNA, and the sex of embryos was clearly identified by both the boiling and extraction method. This simple and rapid method of embryo sexing using blastodermal cells isolated from the area opaca enables us to identify the sex of a large number of chicken embryos in a limited time.
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subjectChick Embryos ; DNA ; Methodology ; Polymerase Chain Reaction ; Poultry ; Sex Diagnosis ; Chickens ; Deoxyribonucleic Acid ; Domesticated Birds ; Methods ; Pcr ; Birds ; Fowls ; Gallus Gallus ; Gallus ; Phasianidae ; Galliformes ; Vertebrates ; Chordata ; Animals ; Eukaryotes;
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titleA Simple Method for Sexing Chicken Embryos at Stage X
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abstract  A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the extraction method (DNA extraction kit) as a control. PCR analysis was carried out using the prepared DNA, and the sex of embryos was clearly identified by both the boiling and extraction method. This simple and rapid method of embryo sexing using blastodermal cells isolated from the area opaca enables us to identify the sex of a large number of chicken embryos in a limited time.
copIbaraki
pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1439290676/
doi10.2141/jpsa.40.226
pages226-230