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Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation

  In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (pbAEGFP) was injected into the stage X blastoderm followed by applying electric pulses vertically to... Full description

Journal Title: The Journal of Poultry Science 2003, Vol.40(4), p.319
Main Author: Sano, Akiko
Other Authors: Harumi, Takashi , Matsubara, Yuko , Naito, Mitsuru
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1439290741/?pq-origsite=primo
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recordid: proquest1439290741
title: Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
format: Article
creator:
  • Sano, Akiko
  • Harumi, Takashi
  • Matsubara, Yuko
  • Naito, Mitsuru
subjects:
  • DNA
  • Electrodes
  • Electroporation
  • Embryo Culture
  • Embryos
  • Gene Expression
  • Genetic Engineering
  • Plasmids
  • Poultry
  • Transfection
  • Chickens
  • Deoxyribonucleic Acid
  • Domesticated Birds
  • Genetic Manipulation
  • Birds
  • Fowls
  • Gallus Gallus
  • Gallus
  • Phasianidae
  • Galliformes
  • Vertebrates
  • Chordata
  • Animals
  • Eukaryotes
ispartof: The Journal of Poultry Science, 2003, Vol.40(4), p.319
description:   In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (pbAEGFP) was injected into the stage X blastoderm followed by applying electric pulses vertically to the blastoderm layer. The manipulated embryos were incubated using an embryo culture system, and the GFP gene expression was then examined. By decreasing the size of the upper electrode or by increasing the size of the lower electrode, the effect of the electric pulses was clearly enhanced. Although the introduced GFP gene was efficiently expressed in the embryonic and extra-embryonic tissues, the expression in the embryos was transient. No integration of the GFP gene into the chromosomes of germline cells of chickens was detected.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


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titleEffect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
creatorSano, Akiko ; Harumi, Takashi ; Matsubara, Yuko ; Naito, Mitsuru
ispartofThe Journal of Poultry Science, 2003, Vol.40(4), p.319
identifierISSN: 13467395 ; E-ISSN: 13490486
description  In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (pbAEGFP) was injected into the stage X blastoderm followed by applying electric pulses vertically to the blastoderm layer. The manipulated embryos were incubated using an embryo culture system, and the GFP gene expression was then examined. By decreasing the size of the upper electrode or by increasing the size of the lower electrode, the effect of the electric pulses was clearly enhanced. Although the introduced GFP gene was efficiently expressed in the embryonic and extra-embryonic tissues, the expression in the embryos was transient. No integration of the GFP gene into the chromosomes of germline cells of chickens was detected.
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subjectDNA ; Electrodes ; Electroporation ; Embryo Culture ; Embryos ; Gene Expression ; Genetic Engineering ; Plasmids ; Poultry ; Transfection ; Chickens ; Deoxyribonucleic Acid ; Domesticated Birds ; Genetic Manipulation ; Birds ; Fowls ; Gallus Gallus ; Gallus ; Phasianidae ; Galliformes ; Vertebrates ; Chordata ; Animals ; Eukaryotes;
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abstract  In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (pbAEGFP) was injected into the stage X blastoderm followed by applying electric pulses vertically to the blastoderm layer. The manipulated embryos were incubated using an embryo culture system, and the GFP gene expression was then examined. By decreasing the size of the upper electrode or by increasing the size of the lower electrode, the effect of the electric pulses was clearly enhanced. Although the introduced GFP gene was efficiently expressed in the embryonic and extra-embryonic tissues, the expression in the embryos was transient. No integration of the GFP gene into the chromosomes of germline cells of chickens was detected.
copIbaraki
pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1439290741/
doi10.2141/jpsa.40.319
pages319-323