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Culture of Chicken Embryos Obtained from the Anterior Region of the Magnum of the Oviduct after Removing a Thin Layer of Dense Albumen Capsule from the Ovum

  Fertilised ova, obtained from the anterior region of the magnum of the oviduct 60-80min after the preceding egg had been laid, were cultured in vitro with dense albumen adjusted to pH 7.0 after removing a thin layer of dense albumen capsule surrounding the ovum. The ova were then cultured in recip... Full description

Journal Title: The Journal of Poultry Science 2005, Vol.42(2), p.140
Main Author: Naito, Mitsuru
Other Authors: Sano, Akiko , Kawashima, Takaharu , Nakamichi, Hiroyuki , Harumi, Takashi , Matsubara, Yuko , Kuwana, Takashi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1439291164/?pq-origsite=primo
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recordid: proquest1439291164
title: Culture of Chicken Embryos Obtained from the Anterior Region of the Magnum of the Oviduct after Removing a Thin Layer of Dense Albumen Capsule from the Ovum
format: Article
creator:
  • Naito, Mitsuru
  • Sano, Akiko
  • Kawashima, Takaharu
  • Nakamichi, Hiroyuki
  • Harumi, Takashi
  • Matsubara, Yuko
  • Kuwana, Takashi
subjects:
  • Chicken Embryo Culture
  • Dense Albumen
  • Embryo Manipulation
  • Fertilised Ovum
ispartof: The Journal of Poultry Science, 2005, Vol.42(2), p.140
description:   Fertilised ova, obtained from the anterior region of the magnum of the oviduct 60-80min after the preceding egg had been laid, were cultured in vitro with dense albumen adjusted to pH 7.0 after removing a thin layer of dense albumen capsule surrounding the ovum. The ova were then cultured in recipient eggshells until hatching. Of 80 cultured embryos, about half developed to the blastoderm stage, 25% survived to day 4 of incubation, and 7.5% survived to hatching. The embryo culture technique developed in this study enables precise manipulation of the germinal disc just after ovulation.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


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titleCulture of Chicken Embryos Obtained from the Anterior Region of the Magnum of the Oviduct after Removing a Thin Layer of Dense Albumen Capsule from the Ovum
creatorNaito, Mitsuru ; Sano, Akiko ; Kawashima, Takaharu ; Nakamichi, Hiroyuki ; Harumi, Takashi ; Matsubara, Yuko ; Kuwana, Takashi
ispartofThe Journal of Poultry Science, 2005, Vol.42(2), p.140
identifierISSN: 13467395 ; E-ISSN: 13490486
description  Fertilised ova, obtained from the anterior region of the magnum of the oviduct 60-80min after the preceding egg had been laid, were cultured in vitro with dense albumen adjusted to pH 7.0 after removing a thin layer of dense albumen capsule surrounding the ovum. The ova were then cultured in recipient eggshells until hatching. Of 80 cultured embryos, about half developed to the blastoderm stage, 25% survived to day 4 of incubation, and 7.5% survived to hatching. The embryo culture technique developed in this study enables precise manipulation of the germinal disc just after ovulation.
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subjectChicken Embryo Culture ; Dense Albumen ; Embryo Manipulation ; Fertilised Ovum;
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abstract  Fertilised ova, obtained from the anterior region of the magnum of the oviduct 60-80min after the preceding egg had been laid, were cultured in vitro with dense albumen adjusted to pH 7.0 after removing a thin layer of dense albumen capsule surrounding the ovum. The ova were then cultured in recipient eggshells until hatching. Of 80 cultured embryos, about half developed to the blastoderm stage, 25% survived to day 4 of incubation, and 7.5% survived to hatching. The embryo culture technique developed in this study enables precise manipulation of the germinal disc just after ovulation.
copIbaraki
pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1439291164/
doi10.2141/jpsa.42.140
pages140-144