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New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers

  Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such test, a new screening test for male putative germline chimeric chickens by polymerase chain react... Full description

Journal Title: The Journal of Poultry Science 2005, Vol.42(2), p.152
Main Author: Sano, Akiko
Other Authors: Harumi, Takashi , Hanzawa, Shozo , Kawashima, Takaharu , Nakamichi, Hiroyuki , Matsubara, Yuko , Naito, Mitsuru
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pcr
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1439291198/?pq-origsite=primo
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title: New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
format: Article
creator:
  • Sano, Akiko
  • Harumi, Takashi
  • Hanzawa, Shozo
  • Kawashima, Takaharu
  • Nakamichi, Hiroyuki
  • Matsubara, Yuko
  • Naito, Mitsuru
subjects:
  • Chimaeras
  • Genetic Polymorphism
  • Germ Line
  • Mitochondrial DNA
  • Nucleotide Sequences
  • Polymerase Chain Reaction
  • Poultry
  • Screening
  • Techniques
  • Chickens
  • Chimeras
  • DNA Sequences
  • Domesticated Birds
  • Pcr
  • Screening Tests
  • Birds
  • Fowls
  • Gallus Gallus
  • Gallus
  • Phasianidae
  • Galliformes
  • Vertebrates
  • Chordata
  • Animals
  • Eukaryotes
ispartof: The Journal of Poultry Science, 2005, Vol.42(2), p.152
description:   Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such test, a new screening test for male putative germline chimeric chickens by polymerase chain reaction (PCR) was developed that uses single nucleotide polymorphism (SNP) detection primers. Putative germline chimeric chickens were produced by the transfer of stage X blastodermal cells or circulating primordial germ cells from Barred Plymouth Rocks (BPR) to White Leghorns (WL). For screening prior to the progeny test, DNA was extracted from the semen and used for PCR analysis, using SNP detection primers at position 686 in the chicken mitochondrial DNA D-loop region (DNA database, AB091008). In this study, the type of SNP in all BPR at position 686 was fixed as base G, and as base A in all WL. When the PCR product was typed as both donor (base G)- and recipient (base A)-derived, the male was determined as the germline chimera. The male and female putative germline chimeric chickens produced in this study were given the progeny test. The results of the male screening test showed good agreement with the progeny test for detecting germline chimeric chickens.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


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titleNew Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
creatorSano, Akiko ; Harumi, Takashi ; Hanzawa, Shozo ; Kawashima, Takaharu ; Nakamichi, Hiroyuki ; Matsubara, Yuko ; Naito, Mitsuru
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identifierISSN: 13467395 ; E-ISSN: 13490486
description  Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such test, a new screening test for male putative germline chimeric chickens by polymerase chain reaction (PCR) was developed that uses single nucleotide polymorphism (SNP) detection primers. Putative germline chimeric chickens were produced by the transfer of stage X blastodermal cells or circulating primordial germ cells from Barred Plymouth Rocks (BPR) to White Leghorns (WL). For screening prior to the progeny test, DNA was extracted from the semen and used for PCR analysis, using SNP detection primers at position 686 in the chicken mitochondrial DNA D-loop region (DNA database, AB091008). In this study, the type of SNP in all BPR at position 686 was fixed as base G, and as base A in all WL. When the PCR product was typed as both donor (base G)- and recipient (base A)-derived, the male was determined as the germline chimera. The male and female putative germline chimeric chickens produced in this study were given the progeny test. The results of the male screening test showed good agreement with the progeny test for detecting germline chimeric chickens.
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subjectChimaeras ; Genetic Polymorphism ; Germ Line ; Mitochondrial DNA ; Nucleotide Sequences ; Polymerase Chain Reaction ; Poultry ; Screening ; Techniques ; Chickens ; Chimeras ; DNA Sequences ; Domesticated Birds ; Pcr ; Screening Tests ; Birds ; Fowls ; Gallus Gallus ; Gallus ; Phasianidae ; Galliformes ; Vertebrates ; Chordata ; Animals ; Eukaryotes;
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titleNew Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
description  Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such test, a new screening test for male putative germline chimeric chickens by polymerase chain reaction (PCR) was developed that uses single nucleotide polymorphism (SNP) detection primers. Putative germline chimeric chickens were produced by the transfer of stage X blastodermal cells or circulating primordial germ cells from Barred Plymouth Rocks (BPR) to White Leghorns (WL). For screening prior to the progeny test, DNA was extracted from the semen and used for PCR analysis, using SNP detection primers at position 686 in the chicken mitochondrial DNA D-loop region (DNA database, AB091008). In this study, the type of SNP in all BPR at position 686 was fixed as base G, and as base A in all WL. When the PCR product was typed as both donor (base G)- and recipient (base A)-derived, the male was determined as the germline chimera. The male and female putative germline chimeric chickens produced in this study were given the progeny test. The results of the male screening test showed good agreement with the progeny test for detecting germline chimeric chickens.
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abstract  Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such test, a new screening test for male putative germline chimeric chickens by polymerase chain reaction (PCR) was developed that uses single nucleotide polymorphism (SNP) detection primers. Putative germline chimeric chickens were produced by the transfer of stage X blastodermal cells or circulating primordial germ cells from Barred Plymouth Rocks (BPR) to White Leghorns (WL). For screening prior to the progeny test, DNA was extracted from the semen and used for PCR analysis, using SNP detection primers at position 686 in the chicken mitochondrial DNA D-loop region (DNA database, AB091008). In this study, the type of SNP in all BPR at position 686 was fixed as base G, and as base A in all WL. When the PCR product was typed as both donor (base G)- and recipient (base A)-derived, the male was determined as the germline chimera. The male and female putative germline chimeric chickens produced in this study were given the progeny test. The results of the male screening test showed good agreement with the progeny test for detecting germline chimeric chickens.
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pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1439291198/
doi10.2141/jpsa.42.152
pages152-157