schliessen

Filtern

 

Bibliotheken

A Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent

  A method of freezing semen using N-methylacetamide (MA) as a cryoprotective agent was applied to Yakido, a chicken breed designated as a "Natural Monument" under the "Law for the Protection of Cultural Property" (Law No. 214, May 30, 1950) in Japan. Semen was collected from Yakido roosters, and po... Full description

Journal Title: The Journal of Poultry Science 2010, Vol.47(4), p.297
Main Author: Sasaki, Kenji
Other Authors: Tatsumi, Toshiaki , Tsutsui, Mariko , Niinomi, Tatsuya , Imai, Takayuki , Naito, Mitsuru , Tajima, Atsushi , Nishi, Yasuhiro
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1439310141/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest1439310141
title: A Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent
format: Article
creator:
  • Sasaki, Kenji
  • Tatsumi, Toshiaki
  • Tsutsui, Mariko
  • Niinomi, Tatsuya
  • Imai, Takayuki
  • Naito, Mitsuru
  • Tajima, Atsushi
  • Nishi, Yasuhiro
subjects:
  • Chicken
  • Cryopreservation
  • Genetic Resources
  • N-Methylacetamide
  • Semen
ispartof: The Journal of Poultry Science, 2010, Vol.47(4), p.297
description:   A method of freezing semen using N-methylacetamide (MA) as a cryoprotective agent was applied to Yakido, a chicken breed designated as a "Natural Monument" under the "Law for the Protection of Cultural Property" (Law No. 214, May 30, 1950) in Japan. Semen was collected from Yakido roosters, and pooled semen was diluted 1: 1 using semen diluent containing no cryoprotective agent. After equilibration at 5°C for 30min, diluted semen was further diluted 1: 1 using semen diluent containing MA at a final concentration of 9%. Diluted semen was packaged into 0.5-mL plastic straws and frozen by placing the straws in liquid nitrogen vapor followed by plunging into liquid nitrogen at -196°C. After storage in liquid nitrogen for about one month, frozen semen was thawed in ice water. Intravaginal artificial insemination (AI) was performed immediately after thawing semen without removing the cryoprotective agent. In Fertility Trial 1, AI was performed once a week for four consecutive weeks. Weekly fertility rates using frozen semen were 83.8±3.8%, 77.1±8.8%, 88.6±5.1%, and 77.3±5.7% for weeks 1, 2, 3, and 4, respectively. Hatchability was over 90% throughout the experimental period. In Fertility Trial 2, the duration of fertility was examined after intravaginal insemination for 2 consecutive days. Daily fertility rates using frozen-thawed semen were 90.0%, 90.0%, 91.7%, 88.9%, 100.0%, 100.0%, 100.0%, 55.6%, 60.0%, 25.0%, 30.0%, 30.0%, and 9.1% for days 1 through 13 after AI, respectively. Overall hatchability was 89.5%. These results indicated that freezing chicken spermatozoa using MA as a cryoprotective agent is a practical method of preserving chicken semen collected from genetically valuable chicken breeds.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


@attributes
ID1431165310
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1439310141
sourceidproquest
recordidTN_proquest1439310141
sourcesystemOther
pqid1439310141
display
typearticle
titleA Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent
creatorSasaki, Kenji ; Tatsumi, Toshiaki ; Tsutsui, Mariko ; Niinomi, Tatsuya ; Imai, Takayuki ; Naito, Mitsuru ; Tajima, Atsushi ; Nishi, Yasuhiro
ispartofThe Journal of Poultry Science, 2010, Vol.47(4), p.297
identifierISSN: 13467395 ; E-ISSN: 13490486
description  A method of freezing semen using N-methylacetamide (MA) as a cryoprotective agent was applied to Yakido, a chicken breed designated as a "Natural Monument" under the "Law for the Protection of Cultural Property" (Law No. 214, May 30, 1950) in Japan. Semen was collected from Yakido roosters, and pooled semen was diluted 1: 1 using semen diluent containing no cryoprotective agent. After equilibration at 5°C for 30min, diluted semen was further diluted 1: 1 using semen diluent containing MA at a final concentration of 9%. Diluted semen was packaged into 0.5-mL plastic straws and frozen by placing the straws in liquid nitrogen vapor followed by plunging into liquid nitrogen at -196°C. After storage in liquid nitrogen for about one month, frozen semen was thawed in ice water. Intravaginal artificial insemination (AI) was performed immediately after thawing semen without removing the cryoprotective agent. In Fertility Trial 1, AI was performed once a week for four consecutive weeks. Weekly fertility rates using frozen semen were 83.8±3.8%, 77.1±8.8%, 88.6±5.1%, and 77.3±5.7% for weeks 1, 2, 3, and 4, respectively. Hatchability was over 90% throughout the experimental period. In Fertility Trial 2, the duration of fertility was examined after intravaginal insemination for 2 consecutive days. Daily fertility rates using frozen-thawed semen were 90.0%, 90.0%, 91.7%, 88.9%, 100.0%, 100.0%, 100.0%, 55.6%, 60.0%, 25.0%, 30.0%, 30.0%, and 9.1% for days 1 through 13 after AI, respectively. Overall hatchability was 89.5%. These results indicated that freezing chicken spermatozoa using MA as a cryoprotective agent is a practical method of preserving chicken semen collected from genetically valuable chicken breeds.
languageeng
source
subjectChicken ; Cryopreservation ; Genetic Resources ; N-Methylacetamide ; Semen;
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/1439310141/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Sasaki, Kenji
1Tatsumi, Toshiaki
2Tsutsui, Mariko
3Niinomi, Tatsuya
4Imai, Takayuki
5Naito, Mitsuru
6Tajima, Atsushi
7Nishi, Yasuhiro
titleA Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent
description  A method of freezing semen using N-methylacetamide (MA) as a cryoprotective agent was applied to Yakido, a chicken breed designated as a "Natural Monument" under the "Law for the Protection of Cultural Property" (Law No. 214, May 30, 1950) in Japan. Semen was collected from Yakido roosters, and pooled semen was diluted 1: 1 using semen diluent containing no cryoprotective agent. After equilibration at 5°C for 30min, diluted semen was further diluted 1: 1 using semen diluent containing MA at a final concentration of 9%. Diluted semen was packaged into 0.5-mL plastic straws and frozen by placing the straws in liquid nitrogen vapor followed by plunging into liquid nitrogen at -196°C. After storage in liquid nitrogen for about one month, frozen semen was thawed in ice water. Intravaginal artificial insemination (AI) was performed immediately after thawing semen without removing the cryoprotective agent. In Fertility Trial 1, AI was performed once a week for four consecutive weeks. Weekly fertility rates using frozen semen were 83.8±3.8%, 77.1±8.8%, 88.6±5.1%, and 77.3±5.7% for weeks 1, 2, 3, and 4, respectively. Hatchability was over 90% throughout the experimental period. In Fertility Trial 2, the duration of fertility was examined after intravaginal insemination for 2 consecutive days. Daily fertility rates using frozen-thawed semen were 90.0%, 90.0%, 91.7%, 88.9%, 100.0%, 100.0%, 100.0%, 55.6%, 60.0%, 25.0%, 30.0%, 30.0%, and 9.1% for days 1 through 13 after AI, respectively. Overall hatchability was 89.5%. These results indicated that freezing chicken spermatozoa using MA as a cryoprotective agent is a practical method of preserving chicken semen collected from genetically valuable chicken breeds.
general
0English
1Japan Science and Technology Agency
2ProQuest Agriculture Journals
3ProQuest Agricultural Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Agricultural & Environmental Science Database
7Natural Science Collection
8SciTech Premium Collection
sourceidproquest
recordidproquest1439310141
issn
013467395
11346-7395
213490486
31349-0486
rsrctypearticle
creationdate2010
addtitleThe Journal of Poultry Science
searchscope
01007160
11007945
21008045
31008046
41008886
51009043
610000035
710000050
810000118
910000120
1010000234
1110000253
1210000260
13proquest
scope
01007160
11007945
21008045
31008046
41008886
51009043
610000035
710000050
810000118
910000120
1010000234
1110000253
1210000260
13proquest
lsr43
01007160false
11007945false
21008045false
31008046false
41008886false
51009043false
610000035false
710000050false
810000118false
910000120false
1010000234false
1110000253false
1210000260false
startdate20101001
enddate20101001
citationpf 297 vol 47 issue 4
lsr30VSR-Enriched:[subject, pages, doi, date, pqid]
sort
titleA Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent
authorSasaki, Kenji ; Tatsumi, Toshiaki ; Tsutsui, Mariko ; Niinomi, Tatsuya ; Imai, Takayuki ; Naito, Mitsuru ; Tajima, Atsushi ; Nishi, Yasuhiro
creationdate20101001
lso0120101001
facets
frbrgroupid8180504852211808793
frbrtype5
languageeng
creationdate2010
collection
0ProQuest Agriculture Journals
1ProQuest Agricultural Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Agricultural & Environmental Science Database
5Natural Science Collection
6SciTech Premium Collection
prefilterarticles
rsrctypearticles
creatorcontrib
0Sasaki, Kenji
1Tatsumi, Toshiaki
2Tsutsui, Mariko
3Niinomi, Tatsuya
4Imai, Takayuki
5Naito, Mitsuru
6Tajima, Atsushi
7Nishi, Yasuhiro
jtitleJournal of Poultry Science
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Sasaki
1Tatsumi
2Tsutsui
3Niinomi
4Imai
5Naito
6Tajima
7Nishi
aufirst
0Kenji
1Toshiaki
2Mariko
3Tatsuya
4Takayuki
5Mitsuru
6Atsushi
7Yasuhiro
auinit1
0K.
1T.
2M.
3A.
4Y.
au
0Sasaki, Kenji
1Tatsumi, Toshiaki
2Tsutsui, Mariko
3Niinomi, Tatsuya
4Imai, Takayuki
5Naito, Mitsuru
6Tajima, Atsushi
7Nishi, Yasuhiro
atitleA Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent
jtitleThe Journal of Poultry Science
date2010
risdate20101001
volume47
issue4
spage297
issn13467395
eissn13490486
formatjournal
genrearticle
ristypeJOUR
abstract  A method of freezing semen using N-methylacetamide (MA) as a cryoprotective agent was applied to Yakido, a chicken breed designated as a "Natural Monument" under the "Law for the Protection of Cultural Property" (Law No. 214, May 30, 1950) in Japan. Semen was collected from Yakido roosters, and pooled semen was diluted 1: 1 using semen diluent containing no cryoprotective agent. After equilibration at 5°C for 30min, diluted semen was further diluted 1: 1 using semen diluent containing MA at a final concentration of 9%. Diluted semen was packaged into 0.5-mL plastic straws and frozen by placing the straws in liquid nitrogen vapor followed by plunging into liquid nitrogen at -196°C. After storage in liquid nitrogen for about one month, frozen semen was thawed in ice water. Intravaginal artificial insemination (AI) was performed immediately after thawing semen without removing the cryoprotective agent. In Fertility Trial 1, AI was performed once a week for four consecutive weeks. Weekly fertility rates using frozen semen were 83.8±3.8%, 77.1±8.8%, 88.6±5.1%, and 77.3±5.7% for weeks 1, 2, 3, and 4, respectively. Hatchability was over 90% throughout the experimental period. In Fertility Trial 2, the duration of fertility was examined after intravaginal insemination for 2 consecutive days. Daily fertility rates using frozen-thawed semen were 90.0%, 90.0%, 91.7%, 88.9%, 100.0%, 100.0%, 100.0%, 55.6%, 60.0%, 25.0%, 30.0%, 30.0%, and 9.1% for days 1 through 13 after AI, respectively. Overall hatchability was 89.5%. These results indicated that freezing chicken spermatozoa using MA as a cryoprotective agent is a practical method of preserving chicken semen collected from genetically valuable chicken breeds.
copIbaraki
pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1439310141/
pages297-301
doi10.2141/jpsa.009111