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Supplemented [Alpha]MEM/F12-based medium enables the survival and growth of primary ovarian follicles encapsulated in alginate hydrogels

Hydrogel-encapsulating culture systems for ovarian follicles support the in vitro growth of secondary follicles from various species including mouse, non-primate human, and human; however, the growth of early stage follicles (primary and primordial) has been limited. While encapsulation maintains th... Full description

Journal Title: Biotechnology and Bioengineering Dec 2013, Vol.110(12), p.3258
Main Author: Tagler, David
Other Authors: Makanji, Yogeshwar , Anderson, Nicholas , Woodruff, Teresa , Shea, Lonnie
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 00063592
Link: http://search.proquest.com/docview/1446977138/?pq-origsite=primo
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title: Supplemented [Alpha]MEM/F12-based medium enables the survival and growth of primary ovarian follicles encapsulated in alginate hydrogels
format: Article
creator:
  • Tagler, David
  • Makanji, Yogeshwar
  • Anderson, Nicholas
  • Woodruff, Teresa
  • Shea, Lonnie
subjects:
  • Rodents
  • Stem Cells
  • Fertility
  • Hydrogels
  • Tissue Engineering
ispartof: Biotechnology and Bioengineering, Dec 2013, Vol.110(12), p.3258
description: Hydrogel-encapsulating culture systems for ovarian follicles support the in vitro growth of secondary follicles from various species including mouse, non-primate human, and human; however, the growth of early stage follicles (primary and primordial) has been limited. While encapsulation maintains the structure of early stage follicles, feeder cell populations, such as mouse embryonic fibroblasts (MEFs), are required to stimulate growth and development. Hence, in this report, we investigated feeder-free culture environments for early stage follicle development. Mouse ovarian follicles were encapsulated within alginate hydrogels and cultured in various growth medium formulations. Initial studies employed embryonic stem cell medium formulations as a tool to identify factors that influence the survival, growth, and meiotic competence of early stage follicles. The medium formulation that maximized survival and growth was identified as ...MEM/F12 supplemented with fetuin, insulin, transferrin, selenium, and follicle stimulating hormone (FSH). This medium stimulated the growth of late primary (average initial diameter of 80 ...m) and early secondary (average initial diameter of 90 ...m) follicles, which developed antral cavities and increased to terminal diameters exceeding 300 ...m in 14 days. Survival ranged from 18% for 80 ...m follicles to 36% for 90 ...m follicles. Furthermore, 80% of the oocytes from surviving follicles with an initial diameter of 90-100 ...m underwent germinal vesicle breakdown (GVBD), and the percentage of metaphase II (MII) eggs was 50%. Follicle/oocyte growth and GVBD/MII rates were not significantly different from MEF co-culture. Survival was reduced relative to MEF co-culture, yet substantially increased relative to the control medium that had been previously used for secondary follicles. Continued development of culture medium could enable mechanistic studies of early stage folliculogenesis and emerging strategies for fertility preservation. (ProQuest: ... denotes formulae/symbols omitted.)
language: eng
source:
identifier: ISSN: 00063592
fulltext: fulltext
issn:
  • 00063592
  • 0006-3592
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titleSupplemented [Alpha]MEM/F12-based medium enables the survival and growth of primary ovarian follicles encapsulated in alginate hydrogels
creatorTagler, David ; Makanji, Yogeshwar ; Anderson, Nicholas ; Woodruff, Teresa ; Shea, Lonnie
ispartofBiotechnology and Bioengineering, Dec 2013, Vol.110(12), p.3258
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subjectRodents ; Stem Cells ; Fertility ; Hydrogels ; Tissue Engineering
descriptionHydrogel-encapsulating culture systems for ovarian follicles support the in vitro growth of secondary follicles from various species including mouse, non-primate human, and human; however, the growth of early stage follicles (primary and primordial) has been limited. While encapsulation maintains the structure of early stage follicles, feeder cell populations, such as mouse embryonic fibroblasts (MEFs), are required to stimulate growth and development. Hence, in this report, we investigated feeder-free culture environments for early stage follicle development. Mouse ovarian follicles were encapsulated within alginate hydrogels and cultured in various growth medium formulations. Initial studies employed embryonic stem cell medium formulations as a tool to identify factors that influence the survival, growth, and meiotic competence of early stage follicles. The medium formulation that maximized survival and growth was identified as ...MEM/F12 supplemented with fetuin, insulin, transferrin, selenium, and follicle stimulating hormone (FSH). This medium stimulated the growth of late primary (average initial diameter of 80 ...m) and early secondary (average initial diameter of 90 ...m) follicles, which developed antral cavities and increased to terminal diameters exceeding 300 ...m in 14 days. Survival ranged from 18% for 80 ...m follicles to 36% for 90 ...m follicles. Furthermore, 80% of the oocytes from surviving follicles with an initial diameter of 90-100 ...m underwent germinal vesicle breakdown (GVBD), and the percentage of metaphase II (MII) eggs was 50%. Follicle/oocyte growth and GVBD/MII rates were not significantly different from MEF co-culture. Survival was reduced relative to MEF co-culture, yet substantially increased relative to the control medium that had been previously used for secondary follicles. Continued development of culture medium could enable mechanistic studies of early stage folliculogenesis and emerging strategies for fertility preservation. (ProQuest: ... denotes formulae/symbols omitted.)
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titleSupplemented [Alpha]MEM/F12-based medium enables the survival and growth of primary ovarian follicles encapsulated in alginate hydrogels
authorTagler, David ; Makanji, Yogeshwar ; Anderson, Nicholas ; Woodruff, Teresa ; Shea, Lonnie
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abstractHydrogel-encapsulating culture systems for ovarian follicles support the in vitro growth of secondary follicles from various species including mouse, non-primate human, and human; however, the growth of early stage follicles (primary and primordial) has been limited. While encapsulation maintains the structure of early stage follicles, feeder cell populations, such as mouse embryonic fibroblasts (MEFs), are required to stimulate growth and development. Hence, in this report, we investigated feeder-free culture environments for early stage follicle development. Mouse ovarian follicles were encapsulated within alginate hydrogels and cultured in various growth medium formulations. Initial studies employed embryonic stem cell medium formulations as a tool to identify factors that influence the survival, growth, and meiotic competence of early stage follicles. The medium formulation that maximized survival and growth was identified as ...MEM/F12 supplemented with fetuin, insulin, transferrin, selenium, and follicle stimulating hormone (FSH). This medium stimulated the growth of late primary (average initial diameter of 80 ...m) and early secondary (average initial diameter of 90 ...m) follicles, which developed antral cavities and increased to terminal diameters exceeding 300 ...m in 14 days. Survival ranged from 18% for 80 ...m follicles to 36% for 90 ...m follicles. Furthermore, 80% of the oocytes from surviving follicles with an initial diameter of 90-100 ...m underwent germinal vesicle breakdown (GVBD), and the percentage of metaphase II (MII) eggs was 50%. Follicle/oocyte growth and GVBD/MII rates were not significantly different from MEF co-culture. Survival was reduced relative to MEF co-culture, yet substantially increased relative to the control medium that had been previously used for secondary follicles. Continued development of culture medium could enable mechanistic studies of early stage folliculogenesis and emerging strategies for fertility preservation. (ProQuest: ... denotes formulae/symbols omitted.)
copNew York
pubWiley Subscription Services, Inc.
urlhttp://search.proquest.com/docview/1446977138/
pages3258
doi10.1002/bit.24986
eissn10970290
date2013-12-01