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Phytoceramide in vertebrate tissues: one step chromatography separation for molecular characterization of ceramide species.

Ceramide is a precursor for complex sphingolipids in vertebrates, while plants contain phytoceramide. By using a novel chromatography purification method we show that phytoceramide comprises a significant proportion of animal sphingolipids. Total ceramide including phytoceramide from mouse tissue (b... Full description

Journal Title: PloS one 2013, Vol.8(11), p.e80841
Main Author: Dasgupta, Somsankar
Other Authors: Kong, Jina , Bieberich, Erhard
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0080841
Link: http://search.proquest.com/docview/1465861174/?pq-origsite=primo
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title: Phytoceramide in vertebrate tissues: one step chromatography separation for molecular characterization of ceramide species.
format: Article
creator:
  • Dasgupta, Somsankar
  • Kong, Jina
  • Bieberich, Erhard
subjects:
  • Animals–Chemistry
  • Ceramides–Metabolism
  • Metabolomics–Methods
  • Mice–Methods
  • Organ Specificity–Methods
  • Vertebrates–Methods
  • Ceramides
ispartof: PloS one, 2013, Vol.8(11), p.e80841
description: Ceramide is a precursor for complex sphingolipids in vertebrates, while plants contain phytoceramide. By using a novel chromatography purification method we show that phytoceramide comprises a significant proportion of animal sphingolipids. Total ceramide including phytoceramide from mouse tissue (brain, heart, liver) lipid extracts and cell culture (mouse primary astrocytes, human oligodendroglioma cells) was eluted as a single homogenous fraction, and then analyzed by thin layer chromatography, and further characterized by gas chromatography-mass spectrometry (GC-MS). We detected a unique band that migrated between non-hydroxy fatty acyl ceramide and hydroxy fatty acyl ceramide, and identified it as phytoceramide. Using RT-PCR, we confirmed that mouse tissues expressed desaturase 2, an enzyme that has been reported to generate phytoceramide from dihydroceramide. Previously, only trace amounts of phytoceramide were reported in vertebrate intestine, kidney, and skin. While its function is still elusive, this is the first report of phytoceramide characterization in glial cells and vertebrate brain, heart, and liver.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0080841
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titlePhytoceramide in vertebrate tissues: one step chromatography separation for molecular characterization of ceramide species.
creatorDasgupta, Somsankar ; Kong, Jina ; Bieberich, Erhard
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0080841
subjectAnimals–Chemistry ; Ceramides–Metabolism ; Metabolomics–Methods ; Mice–Methods ; Organ Specificity–Methods ; Vertebrates–Methods ; Ceramides
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descriptionCeramide is a precursor for complex sphingolipids in vertebrates, while plants contain phytoceramide. By using a novel chromatography purification method we show that phytoceramide comprises a significant proportion of animal sphingolipids. Total ceramide including phytoceramide from mouse tissue (brain, heart, liver) lipid extracts and cell culture (mouse primary astrocytes, human oligodendroglioma cells) was eluted as a single homogenous fraction, and then analyzed by thin layer chromatography, and further characterized by gas chromatography-mass spectrometry (GC-MS). We detected a unique band that migrated between non-hydroxy fatty acyl ceramide and hydroxy fatty acyl ceramide, and identified it as phytoceramide. Using RT-PCR, we confirmed that mouse tissues expressed desaturase 2, an enzyme that has been reported to generate phytoceramide from dihydroceramide. Previously, only trace amounts of phytoceramide were reported in vertebrate intestine, kidney, and skin. While its function is still elusive, this is the first report of phytoceramide characterization in glial cells and vertebrate brain, heart, and liver.
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