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Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth

Biologically derived fuels are viable alternatives to traditional fossil fuels, and microalgae are a particularly promising source, but improvements are required throughout the production process to increase productivity and reduce cost. Metabolic engineering to increase yields of biofuel-relevant l... Full description

Journal Title: Proceedings of the National Academy of Sciences USA, December 2013, Vol.110(49), p.19748
Main Author: Trentacoste, Emily
Other Authors: Shrestha, Roshan , Smith, Sarah , Gle, Corine , Hartmann, Aaron , Hildebrand, Mark , Gerwick, William
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0027-8424
Link: http://search.proquest.com/docview/1492629784/?pq-origsite=primo
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title: Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth
format: Article
creator:
  • Trentacoste, Emily
  • Shrestha, Roshan
  • Smith, Sarah
  • Gle, Corine
  • Hartmann, Aaron
  • Hildebrand, Mark
  • Gerwick, William
subjects:
  • Silicon
  • Economic Feasibility
  • Growth
  • Fossil Fuels
  • Lipids
  • Fatty Acids
  • Strains
  • Metabolism
  • Algae
  • Starvation
  • Silicon
  • Fossil Fuels
  • Fuels
  • Metabolic Engineering
  • Diatoms
  • Enzymes
  • Phospholipase
  • Homeostasis
  • Lipid Turnover
  • Lipid Metabolism
  • Light Effects
  • Triacylglycerol Lipase
  • Acyltransferase
  • Economics
  • Fatty Acids
  • Thalassiosira Pseudonana
  • Cell Biology
  • Non-Edible Products
ispartof: Proceedings of the National Academy of Sciences, USA, December 2013, Vol.110(49), p.19748
description: Biologically derived fuels are viable alternatives to traditional fossil fuels, and microalgae are a particularly promising source, but improvements are required throughout the production process to increase productivity and reduce cost. Metabolic engineering to increase yields of biofuel-relevant lipids in these organisms without compromising growth is an important aspect of advancing economic feasibility. We report that the targeted knockdown of a multifunctional lipase/phospholipase/acyltransferase increased lipid yields without affecting growth in the diatom Thalassiosira pseudonana. Antisense-expressing knockdown strains 1A6 and 1B1 exhibited wild-type-like growth and increased lipid content under both continuous light and alternating light/dark conditions. Strains 1A6 and 1B1, respectively, contained 2.4- and 3.3-fold higher lipid content than wild-type during exponential growth, and 4.1- and 3.2-fold higher lipid content than wild-type after 40 h of silicon starvation. Analyses of fatty acids, lipid classes, and membrane stability in the transgenic strains suggest a role for this enzyme in membrane lipid turnover and lipid homeostasis. These results demonstrate that targeted metabolic manipulations can be used to increase lipid accumulation in eukaryotic microalgae without compromising growth.
language: eng
source:
identifier: ISSN: 0027-8424
fulltext: fulltext
issn:
  • 00278424
  • 0027-8424
url: Link


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titleMetabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth
creatorTrentacoste, Emily ; Shrestha, Roshan ; Smith, Sarah ; Gle, Corine ; Hartmann, Aaron ; Hildebrand, Mark ; Gerwick, William
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ispartofProceedings of the National Academy of Sciences, USA, December 2013, Vol.110(49), p.19748
identifierISSN: 0027-8424
subjectSilicon ; Economic Feasibility ; Growth ; Fossil Fuels ; Lipids ; Fatty Acids ; Strains ; Metabolism ; Algae ; Starvation ; Silicon ; Fossil Fuels ; Fuels ; Metabolic Engineering ; Diatoms ; Enzymes ; Phospholipase ; Homeostasis ; Lipid Turnover ; Lipid Metabolism ; Light Effects ; Triacylglycerol Lipase ; Acyltransferase ; Economics ; Fatty Acids ; Thalassiosira Pseudonana ; Cell Biology ; Non-Edible Products
descriptionBiologically derived fuels are viable alternatives to traditional fossil fuels, and microalgae are a particularly promising source, but improvements are required throughout the production process to increase productivity and reduce cost. Metabolic engineering to increase yields of biofuel-relevant lipids in these organisms without compromising growth is an important aspect of advancing economic feasibility. We report that the targeted knockdown of a multifunctional lipase/phospholipase/acyltransferase increased lipid yields without affecting growth in the diatom Thalassiosira pseudonana. Antisense-expressing knockdown strains 1A6 and 1B1 exhibited wild-type-like growth and increased lipid content under both continuous light and alternating light/dark conditions. Strains 1A6 and 1B1, respectively, contained 2.4- and 3.3-fold higher lipid content than wild-type during exponential growth, and 4.1- and 3.2-fold higher lipid content than wild-type after 40 h of silicon starvation. Analyses of fatty acids, lipid classes, and membrane stability in the transgenic strains suggest a role for this enzyme in membrane lipid turnover and lipid homeostasis. These results demonstrate that targeted metabolic manipulations can be used to increase lipid accumulation in eukaryotic microalgae without compromising growth.
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titleMetabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth
descriptionBiologically derived fuels are viable alternatives to traditional fossil fuels, and microalgae are a particularly promising source, but improvements are required throughout the production process to increase productivity and reduce cost. Metabolic engineering to increase yields of biofuel-relevant lipids in these organisms without compromising growth is an important aspect of advancing economic feasibility. We report that the targeted knockdown of a multifunctional lipase/phospholipase/acyltransferase increased lipid yields without affecting growth in the diatom Thalassiosira pseudonana. Antisense-expressing knockdown strains 1A6 and 1B1 exhibited wild-type-like growth and increased lipid content under both continuous light and alternating light/dark conditions. Strains 1A6 and 1B1, respectively, contained 2.4- and 3.3-fold higher lipid content than wild-type during exponential growth, and 4.1- and 3.2-fold higher lipid content than wild-type after 40 h of silicon starvation. Analyses of fatty acids, lipid classes, and membrane stability in the transgenic strains suggest a role for this enzyme in membrane lipid turnover and lipid homeostasis. These results demonstrate that targeted metabolic manipulations can be used to increase lipid accumulation in eukaryotic microalgae without compromising growth.
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titleMetabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth
authorTrentacoste, Emily ; Shrestha, Roshan ; Smith, Sarah ; Gle, Corine ; Hartmann, Aaron ; Hildebrand, Mark ; Gerwick, William
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abstractBiologically derived fuels are viable alternatives to traditional fossil fuels, and microalgae are a particularly promising source, but improvements are required throughout the production process to increase productivity and reduce cost. Metabolic engineering to increase yields of biofuel-relevant lipids in these organisms without compromising growth is an important aspect of advancing economic feasibility. We report that the targeted knockdown of a multifunctional lipase/phospholipase/acyltransferase increased lipid yields without affecting growth in the diatom Thalassiosira pseudonana. Antisense-expressing knockdown strains 1A6 and 1B1 exhibited wild-type-like growth and increased lipid content under both continuous light and alternating light/dark conditions. Strains 1A6 and 1B1, respectively, contained 2.4- and 3.3-fold higher lipid content than wild-type during exponential growth, and 4.1- and 3.2-fold higher lipid content than wild-type after 40 h of silicon starvation. Analyses of fatty acids, lipid classes, and membrane stability in the transgenic strains suggest a role for this enzyme in membrane lipid turnover and lipid homeostasis. These results demonstrate that targeted metabolic manipulations can be used to increase lipid accumulation in eukaryotic microalgae without compromising growth.
urlhttp://search.proquest.com/docview/1492629784/
doi10.1073/pnas.1309299110
eissn10916490
date2013-12-03