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Escherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts.

The biology of Escherichia coli in its primary niche, the animal intestinal tract, is remarkably unexplored. Studies with the streptomycin-treated mouse model have produced important insights into the metabolic requirements for Escherichia coli to colonize mice. However, we still know relatively lit... Full description

Journal Title: Journal of bacteriology May 2014, Vol.196(9), pp.1723-1732
Main Author: Lasaro, Melissa
Other Authors: Liu, Zhi , Bishar, Rima , Kelly, Kathryn , Chattopadhyay, Sujay , Paul, Sandip , Sokurenko, Evgeni , Zhu, Jun , Goulian, Mark
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1098-5530 ; DOI: 10.1128/JB.01296-13
Link: http://search.proquest.com/docview/1513049231/?pq-origsite=primo
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recordid: proquest1513049231
title: Escherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts.
format: Article
creator:
  • Lasaro, Melissa
  • Liu, Zhi
  • Bishar, Rima
  • Kelly, Kathryn
  • Chattopadhyay, Sujay
  • Paul, Sandip
  • Sokurenko, Evgeni
  • Zhu, Jun
  • Goulian, Mark
subjects:
  • Animals–Genetics
  • Bacterial Outer Membrane Proteins–Metabolism
  • Bacterial Proteins–Genetics
  • Escherichia Coli–Metabolism
  • Escherichia Coli Proteins–Genetics
  • Gene Knockout Techniques–Growth & Development
  • Intestinal Mucosa–Isolation & Purification
  • Mice–Metabolism
  • Repressor Proteins–Genetics
  • Transcription Factors–Metabolism
  • Transcription Factors–Microbiology
  • Transcription Factors–Genetics
  • Transcription Factors–Metabolism
  • Transcription Factors–Genetics
  • Transcription Factors–Metabolism
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Escherichia Coli Proteins
  • Rcsb Protein, E Coli
  • Repressor Proteins
  • Transcription Factors
  • Arca Protein, E Coli
  • Cpxr Protein, Bacteria
ispartof: Journal of bacteriology, May 2014, Vol.196(9), pp.1723-1732
description: The biology of Escherichia coli in its primary niche, the animal intestinal tract, is remarkably unexplored. Studies with the streptomycin-treated mouse model have produced important insights into the metabolic requirements for Escherichia coli to colonize mice. However, we still know relatively little about the physiology of this bacterium growing in the complex environment of an intestine that is permissive for the growth of competing flora. We have developed a system for studying colonization using an E. coli strain, MP1, isolated from a mouse. MP1 is genetically tractable and does not require continuous antibiotic treatment for stable colonization. As an application of this system, we separately knocked out each two-component system response regulator in MP1 and performed competitions against the wild-type strain. We found that only three response regulators, ArcA, CpxR, and RcsB, produce strong colonization defects, suggesting that in addition to anaerobiosis, adaptation to cell envelope stress is a critical requirement for E. coli colonization of the mouse intestine. We also show that the response regulator OmpR, which had previously been hypothesized to be important for adaptation between in vivo and ex vivo environments, is not required for MP1 colonization due to the presence of a third major porin. ; p. 1723-1732.
language: eng
source:
identifier: E-ISSN: 1098-5530 ; DOI: 10.1128/JB.01296-13
fulltext: fulltext
issn:
  • 10985530
  • 1098-5530
url: Link


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titleEscherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts.
creatorLasaro, Melissa ; Liu, Zhi ; Bishar, Rima ; Kelly, Kathryn ; Chattopadhyay, Sujay ; Paul, Sandip ; Sokurenko, Evgeni ; Zhu, Jun ; Goulian, Mark
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identifierE-ISSN: 1098-5530 ; DOI: 10.1128/JB.01296-13
subjectAnimals–Genetics ; Bacterial Outer Membrane Proteins–Metabolism ; Bacterial Proteins–Genetics ; Escherichia Coli–Metabolism ; Escherichia Coli Proteins–Genetics ; Gene Knockout Techniques–Growth & Development ; Intestinal Mucosa–Isolation & Purification ; Mice–Metabolism ; Repressor Proteins–Genetics ; Transcription Factors–Metabolism ; Transcription Factors–Microbiology ; Transcription Factors–Genetics ; Transcription Factors–Metabolism ; Transcription Factors–Genetics ; Transcription Factors–Metabolism ; Bacterial Outer Membrane Proteins ; Bacterial Proteins ; Escherichia Coli Proteins ; Rcsb Protein, E Coli ; Repressor Proteins ; Transcription Factors ; Arca Protein, E Coli ; Cpxr Protein, Bacteria
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descriptionThe biology of Escherichia coli in its primary niche, the animal intestinal tract, is remarkably unexplored. Studies with the streptomycin-treated mouse model have produced important insights into the metabolic requirements for Escherichia coli to colonize mice. However, we still know relatively little about the physiology of this bacterium growing in the complex environment of an intestine that is permissive for the growth of competing flora. We have developed a system for studying colonization using an E. coli strain, MP1, isolated from a mouse. MP1 is genetically tractable and does not require continuous antibiotic treatment for stable colonization. As an application of this system, we separately knocked out each two-component system response regulator in MP1 and performed competitions against the wild-type strain. We found that only three response regulators, ArcA, CpxR, and RcsB, produce strong colonization defects, suggesting that in addition to anaerobiosis, adaptation to cell envelope stress is a critical requirement for E. coli colonization of the mouse intestine. We also show that the response regulator OmpR, which had previously been hypothesized to be important for adaptation between in vivo and ex vivo environments, is not required for MP1 colonization due to the presence of a third major porin. ; p. 1723-1732.
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titleEscherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts.
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