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Virus-encapsulated DNA origami nanostructures for cellular delivery.

DNA origami structures can be programmed into arbitrary shapes with nanometer scale precision, which opens up numerous attractive opportunities to engineer novel functional materials. One intriguing possibility is to use DNA origamis for fully tunable, targeted, and triggered drug delivery. In this... Full description

Journal Title: Nano letters 2014, Vol.14(4), pp.2196-2200
Main Author: Mikkilä, Joona
Other Authors: Eskelinen, Antti-Pekka , Niemelä, Elina H , Linko, Veikko , Frilander, Mikko J , Törmä, Päivi , Kostiainen, Mauri A
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: E-ISSN: 1530-6992 ; DOI: 1530-6992 ; DOI: 10.1021/nl500677j
Link: http://search.proquest.com/docview/1514433187/?pq-origsite=primo
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recordid: proquest1514433187
title: Virus-encapsulated DNA origami nanostructures for cellular delivery.
format: Article
creator:
  • Mikkilä, Joona
  • Eskelinen, Antti-Pekka
  • Niemelä, Elina H
  • Linko, Veikko
  • Frilander, Mikko J
  • Törmä, Päivi
  • Kostiainen, Mauri A
subjects:
  • Capsid Proteins–Chemistry
  • DNA–Metabolism
  • Drug Delivery Systems–Administration & Dosage
  • Hek293 Cells–Chemistry
  • Humans–Genetics
  • Immobilized Proteins–Chemistry
  • Models, Molecular–Metabolism
  • Nanostructures–Chemistry
  • Nucleic Acid Conformation–Chemistry
  • Transfection–Chemistry
  • Capsid Proteins
  • Immobilized Proteins
  • DNA
ispartof: Nano letters, 2014, Vol.14(4), pp.2196-2200
description: DNA origami structures can be programmed into arbitrary shapes with nanometer scale precision, which opens up numerous attractive opportunities to engineer novel functional materials. One intriguing possibility is to use DNA origamis for fully tunable, targeted, and triggered drug delivery. In this work, we demonstrate the coating of DNA origami nanostructures with virus capsid proteins for enhancing cellular delivery. Our approach utilizes purified cowpea chlorotic mottle virus capsid proteins that can bind and self-assemble on the origami surface through electrostatic interactions and further pack the origami nanostructures inside the viral capsid. Confocal microscopy imaging and transfection studies with a human HEK293 cell line indicate that protein coating improves cellular attachment and delivery of origamis into the cells by 13-fold compared to bare DNA origamis. The presented method could readily find applications not only in sophisticated drug delivery applications but also in organizing intracellular reactions by origami-based templates. Keywords: DNA nanotechnology; self-assembly; virus capsid protein; cellular delivery; cell uptake
language: eng
source:
identifier: E-ISSN: 1530-6992 ; DOI: 1530-6992 ; DOI: 10.1021/nl500677j
fulltext: no_fulltext
issn:
  • 15306992
  • 1530-6992
url: Link


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titleVirus-encapsulated DNA origami nanostructures for cellular delivery.
creatorMikkilä, Joona ; Eskelinen, Antti-Pekka ; Niemelä, Elina H ; Linko, Veikko ; Frilander, Mikko J ; Törmä, Päivi ; Kostiainen, Mauri A
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subjectCapsid Proteins–Chemistry ; DNA–Metabolism ; Drug Delivery Systems–Administration & Dosage ; Hek293 Cells–Chemistry ; Humans–Genetics ; Immobilized Proteins–Chemistry ; Models, Molecular–Metabolism ; Nanostructures–Chemistry ; Nucleic Acid Conformation–Chemistry ; Transfection–Chemistry ; Capsid Proteins ; Immobilized Proteins ; DNA
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descriptionDNA origami structures can be programmed into arbitrary shapes with nanometer scale precision, which opens up numerous attractive opportunities to engineer novel functional materials. One intriguing possibility is to use DNA origamis for fully tunable, targeted, and triggered drug delivery. In this work, we demonstrate the coating of DNA origami nanostructures with virus capsid proteins for enhancing cellular delivery. Our approach utilizes purified cowpea chlorotic mottle virus capsid proteins that can bind and self-assemble on the origami surface through electrostatic interactions and further pack the origami nanostructures inside the viral capsid. Confocal microscopy imaging and transfection studies with a human HEK293 cell line indicate that protein coating improves cellular attachment and delivery of origamis into the cells by 13-fold compared to bare DNA origamis. The presented method could readily find applications not only in sophisticated drug delivery applications but also in organizing intracellular reactions by origami-based templates. Keywords: DNA nanotechnology; self-assembly; virus capsid protein; cellular delivery; cell uptake
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