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Influence of Valine Analogues on Protein Synthesis of Chicken Embryo Myoblasts

  The influence of various valine analogues on protein synthesis of chicken embryo myoblasts was examined. Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS... Full description

Journal Title: The Journal of Poultry Science 2014, Vol.51(2), p.191
Main Author: Kita, Kazumi
Other Authors: Makino, Ryosuke
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 13467395 ; E-ISSN: 13490486
Link: http://search.proquest.com/docview/1520849626/?pq-origsite=primo
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recordid: proquest1520849626
title: Influence of Valine Analogues on Protein Synthesis of Chicken Embryo Myoblasts
format: Article
creator:
  • Kita, Kazumi
  • Makino, Ryosuke
subjects:
  • Analogues
  • Branched Chain Amino Acids
  • Myoblast
  • Protein Synthesis
  • Valine
  • Valinol
ispartof: The Journal of Poultry Science, 2014, Vol.51(2), p.191
description:   The influence of various valine analogues on protein synthesis of chicken embryo myoblasts was examined. Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). Influence of branched chain amino acids (isoleucine, leucine, valine) on myoblast protein synthesis was also examined. The levels of valine analogue concentration were based on the valine concentration (213 μM) in Medium 199, and the concentrations of supplements were set at 213 μM (×1 of valine in Medium 199) and 2,130 μM (×10 of valine in Medium 199). Protein synthesis was measured by incorporation of 3H-phenylalanine. Methylvaline, which is one of valine derivatives having a methylated amino group, and D-valine, which is an optical isomer of L-valine, had no influence on myoblast protein synthesis although the concentrations of these compounds were 10-times higher (2,130 μM) than that of valine in Medium 199. Leucine and valine had the potency to increase protein synthesis of chicken embryo myoblasts. The supplementation of valinol, which is one of valine derivatives having a hydrated carboxyl group, with 10-times higher concentration than that of valine in Medium 199, decreased myoblast protein synthesis. These results suggested that valinol could be used as an inhibitor of myoblast protein synthesis.
language: eng
source:
identifier: ISSN: 13467395 ; E-ISSN: 13490486
fulltext: fulltext
issn:
  • 13467395
  • 1346-7395
  • 13490486
  • 1349-0486
url: Link


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titleInfluence of Valine Analogues on Protein Synthesis of Chicken Embryo Myoblasts
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ispartofThe Journal of Poultry Science, 2014, Vol.51(2), p.191
identifierISSN: 13467395 ; E-ISSN: 13490486
description  The influence of various valine analogues on protein synthesis of chicken embryo myoblasts was examined. Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). Influence of branched chain amino acids (isoleucine, leucine, valine) on myoblast protein synthesis was also examined. The levels of valine analogue concentration were based on the valine concentration (213 μM) in Medium 199, and the concentrations of supplements were set at 213 μM (×1 of valine in Medium 199) and 2,130 μM (×10 of valine in Medium 199). Protein synthesis was measured by incorporation of 3H-phenylalanine. Methylvaline, which is one of valine derivatives having a methylated amino group, and D-valine, which is an optical isomer of L-valine, had no influence on myoblast protein synthesis although the concentrations of these compounds were 10-times higher (2,130 μM) than that of valine in Medium 199. Leucine and valine had the potency to increase protein synthesis of chicken embryo myoblasts. The supplementation of valinol, which is one of valine derivatives having a hydrated carboxyl group, with 10-times higher concentration than that of valine in Medium 199, decreased myoblast protein synthesis. These results suggested that valinol could be used as an inhibitor of myoblast protein synthesis.
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subjectAnalogues ; Branched Chain Amino Acids ; Myoblast ; Protein Synthesis ; Valine ; Valinol;
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abstract  The influence of various valine analogues on protein synthesis of chicken embryo myoblasts was examined. Valine and its analogues (D-valine, methylvaline, valinol) were supplemented into Medium 199 containing 20 ng/ml of chicken insulin-like growth factor-I (IGF-I) instead of fetal calf serum (FCS). Influence of branched chain amino acids (isoleucine, leucine, valine) on myoblast protein synthesis was also examined. The levels of valine analogue concentration were based on the valine concentration (213 μM) in Medium 199, and the concentrations of supplements were set at 213 μM (×1 of valine in Medium 199) and 2,130 μM (×10 of valine in Medium 199). Protein synthesis was measured by incorporation of 3H-phenylalanine. Methylvaline, which is one of valine derivatives having a methylated amino group, and D-valine, which is an optical isomer of L-valine, had no influence on myoblast protein synthesis although the concentrations of these compounds were 10-times higher (2,130 μM) than that of valine in Medium 199. Leucine and valine had the potency to increase protein synthesis of chicken embryo myoblasts. The supplementation of valinol, which is one of valine derivatives having a hydrated carboxyl group, with 10-times higher concentration than that of valine in Medium 199, decreased myoblast protein synthesis. These results suggested that valinol could be used as an inhibitor of myoblast protein synthesis.
copIbaraki
pubJapan Science and Technology Agency
urlhttp://search.proquest.com/docview/1520849626/
pages191-194
doi10.2141/jpsa.0130162
date2014-04-01