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Pepper suppressor of the G2 allele of skp1 interacts with the receptor-like cytoplasmic kinase1 and type III effector AvrBsT and promotes the hypersensitive cell death response in a phosphorylation-dependent manner.

Xanthomonas campestris pv vesicatoria type III effector protein, AvrBsT, triggers hypersensitive cell death in pepper (Capsicum annuum). Here, we have identified the pepper SGT1 (for suppressor of the G2 alíele of skpl) as a host interactor of AvrBsT and also the pepper PIK1 (for receptor-like cytop... Full description

Journal Title: Plant physiology May 2014, Vol.165(1), pp.76-91
Main Author: Kim, Nak Hyun
Other Authors: Kim, Dae Sung , Chung, Eui Hwan , Hwang, Byung Kook
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1532-2548 ; DOI: 10.1104/pp.114.238840
Link: http://search.proquest.com/docview/1521911829/?pq-origsite=primo
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title: Pepper suppressor of the G2 allele of skp1 interacts with the receptor-like cytoplasmic kinase1 and type III effector AvrBsT and promotes the hypersensitive cell death response in a phosphorylation-dependent manner.
format: Article
creator:
  • Kim, Nak Hyun
  • Kim, Dae Sung
  • Chung, Eui Hwan
  • Hwang, Byung Kook
subjects:
  • Alleles–Metabolism
  • Amino Acid Sequence–Cytology
  • Bacterial Proteins–Genetics
  • Bacterial Secretion Systems–Immunology
  • Capsicum–Microbiology
  • Cell Death–Metabolism
  • Cell Nucleus–Metabolism
  • Chromatography, Liquid–Genetics
  • Gene Expression Regulation, Plant–Metabolism
  • Gene Silencing–Chemistry
  • Molecular Sequence Data–Metabolism
  • Multiprotein Complexes–Metabolism
  • Mutation–Metabolism
  • Phosphorylation–Metabolism
  • Phosphoserine–Metabolism
  • Plant Proteins–Metabolism
  • Protein Binding–Metabolism
  • Protein Transport–Metabolism
  • Saccharomyces Cerevisiae–Metabolism
  • Tandem Mass Spectrometry
ispartof: Plant physiology, May 2014, Vol.165(1), pp.76-91
description: Xanthomonas campestris pv vesicatoria type III effector protein, AvrBsT, triggers hypersensitive cell death in pepper (Capsicum annuum). Here, we have identified the pepper SGT1 (for suppressor of the G2 alíele of skpl) as a host interactor of AvrBsT and also the pepper PIK1 (for receptor-like cytoplasmic kinasel). PIK1 specifically phosphorylates SGT1 and AvrBsT in vitro. AvrBsT specifically binds to the CHORD-containing protein and SGT1 domain of SGT1, resulting in the inhibition of PIK1-mediated SGT1 phosphorylation and subsequent nuclear transport of the SGT1-PIK1 complex. Liquid chromatography-tandem mass spectrometry of the proteolytic peptides of SGT1 identified the residues serine-98 and serine-279 of SGT1 as the major PIK1-mediated phosphorylation sites. Site-directed mutagenesis of SGT1 revealed that the identified SGT1 phosphorylation sites are responsible for the activation of AvrBsT-triggered cell death in planta. SGT1 forms a heterotrimeric complex with both AvrBsT and PIK1 exclusively in the cytoplasm. Agrohacterium tumefaciens-mediated coexpression of SGT1 and PIK1 with avrBsT promotes avrBs T-triggered cell death in Nicotiana benthamiana, dependent on PIK1. Virus-induced silencing of SGT1 and/or PIK1 compromises avrBs T-triggered cell death, hydrogen peroxide production, defense gene induction, and salicylic acid accumulation, leading to the enhanced bacterial pathogen growth in pepper. Together, these results suggest that SGT1 interacts with PIK1 and the bacterial effector protein AvrBsT and promotes the hypersensitive cell death associated with PIK1 -mediated phosphorylation in plants.
language: eng
source:
identifier: E-ISSN: 1532-2548 ; DOI: 10.1104/pp.114.238840
fulltext: fulltext
issn:
  • 15322548
  • 1532-2548
url: Link


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titlePepper suppressor of the G2 allele of skp1 interacts with the receptor-like cytoplasmic kinase1 and type III effector AvrBsT and promotes the hypersensitive cell death response in a phosphorylation-dependent manner.
creatorKim, Nak Hyun ; Kim, Dae Sung ; Chung, Eui Hwan ; Hwang, Byung Kook
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ispartofPlant physiology, May 2014, Vol.165(1), pp.76-91
identifierE-ISSN: 1532-2548 ; DOI: 10.1104/pp.114.238840
subjectAlleles–Metabolism ; Amino Acid Sequence–Cytology ; Bacterial Proteins–Genetics ; Bacterial Secretion Systems–Immunology ; Capsicum–Microbiology ; Cell Death–Metabolism ; Cell Nucleus–Metabolism ; Chromatography, Liquid–Genetics ; Gene Expression Regulation, Plant–Metabolism ; Gene Silencing–Chemistry ; Molecular Sequence Data–Metabolism ; Multiprotein Complexes–Metabolism ; Mutation–Metabolism ; Phosphorylation–Metabolism ; Phosphoserine–Metabolism ; Plant Proteins–Metabolism ; Protein Binding–Metabolism ; Protein Transport–Metabolism ; Saccharomyces Cerevisiae–Metabolism ; Tandem Mass Spectrometry
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descriptionXanthomonas campestris pv vesicatoria type III effector protein, AvrBsT, triggers hypersensitive cell death in pepper (Capsicum annuum). Here, we have identified the pepper SGT1 (for suppressor of the G2 alíele of skpl) as a host interactor of AvrBsT and also the pepper PIK1 (for receptor-like cytoplasmic kinasel). PIK1 specifically phosphorylates SGT1 and AvrBsT in vitro. AvrBsT specifically binds to the CHORD-containing protein and SGT1 domain of SGT1, resulting in the inhibition of PIK1-mediated SGT1 phosphorylation and subsequent nuclear transport of the SGT1-PIK1 complex. Liquid chromatography-tandem mass spectrometry of the proteolytic peptides of SGT1 identified the residues serine-98 and serine-279 of SGT1 as the major PIK1-mediated phosphorylation sites. Site-directed mutagenesis of SGT1 revealed that the identified SGT1 phosphorylation sites are responsible for the activation of AvrBsT-triggered cell death in planta. SGT1 forms a heterotrimeric complex with both AvrBsT and PIK1 exclusively in the cytoplasm. Agrohacterium tumefaciens-mediated coexpression of SGT1 and PIK1 with avrBsT promotes avrBs T-triggered cell death in Nicotiana benthamiana, dependent on PIK1. Virus-induced silencing of SGT1 and/or PIK1 compromises avrBs T-triggered cell death, hydrogen peroxide production, defense gene induction, and salicylic acid accumulation, leading to the enhanced bacterial pathogen growth in pepper. Together, these results suggest that SGT1 interacts with PIK1 and the bacterial effector protein AvrBsT and promotes the hypersensitive cell death associated with PIK1 -mediated phosphorylation in plants.
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titlePepper suppressor of the G2 allele of skp1 interacts with the receptor-like cytoplasmic kinase1 and type III effector AvrBsT and promotes the hypersensitive cell death response in a phosphorylation-dependent manner.
authorKim, Nak Hyun ; Kim, Dae Sung ; Chung, Eui Hwan ; Hwang, Byung Kook
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13Phosphorylation–Metabolism
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15Plant Proteins–Metabolism
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