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GLP-1 ameliorates the proliferation activity of INS-1 cells inhibited by intermittent high glucose concentrations through the regulation of cyclins.

Glucagon-like peptide-1 (GLP-1) and its analog exendin (EX)-4 have been considered to promote [beta]-cell growth and expansion. In the present, study we investigated the effect of GLP-1 on proliferative activity and cell cycle regulation in the pancreatic insulin-secreting [beta]-cell line, INS-1, t... Full description

Journal Title: Molecular medicine reports August 2014, Vol.10(2), pp.683-688
Main Author: Zhang, Zhen
Other Authors: Li, Jing , Jiang, Xinkui , Yang, Lei , Lei, Lei , Cai, Dehong , Zhang, Hua , Chen, Hong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2014.2265
Link: http://search.proquest.com/docview/1535629052/?pq-origsite=primo
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title: GLP-1 ameliorates the proliferation activity of INS-1 cells inhibited by intermittent high glucose concentrations through the regulation of cyclins.
format: Article
creator:
  • Zhang, Zhen
  • Li, Jing
  • Jiang, Xinkui
  • Yang, Lei
  • Lei, Lei
  • Cai, Dehong
  • Zhang, Hua
  • Chen, Hong
subjects:
  • Animals–Drug Effects
  • Cell Line, Tumor–Metabolism
  • Cell Proliferation–Metabolism
  • Cyclin D1–Metabolism
  • Cyclin-Dependent Kinase Inhibitor P21–Metabolism
  • Cyclin-Dependent Kinase Inhibitor P27–Drug Effects
  • Cyclins–Pharmacology
  • G1 Phase Cell Cycle Checkpoints–Pharmacology
  • Glucagon-Like Peptide 1–Metabolism
  • Glucose–Metabolism
  • Rats–Metabolism
  • S-Phase Kinase-Associated Proteins–Metabolism
  • Cyclin-Dependent Kinase Inhibitor P21
  • Cyclins
  • S-Phase Kinase-Associated Proteins
  • Cyclin D1
  • Cyclin-Dependent Kinase Inhibitor P27
  • Glucagon-Like Peptide 1
  • Glucose
ispartof: Molecular medicine reports, August 2014, Vol.10(2), pp.683-688
description: Glucagon-like peptide-1 (GLP-1) and its analog exendin (EX)-4 have been considered to promote [beta]-cell growth and expansion. In the present, study we investigated the effect of GLP-1 on proliferative activity and cell cycle regulation in the pancreatic insulin-secreting [beta]-cell line, INS-1, treated with intermittent high glucose. INS-1 cells were treated with normal glucose (5.5 mmol/l), constant high glucose (30 mmol/l) and intermittent high glucose (rotation/24 h in 5.5 or 30 mmol/l) in the presence or absence of GLP-1 (100 nmol/l) for seven days. Proliferative activity, cell cycle and the expression of cyclin D1, p21, p27 and Skp2 were examined. INS-1 treated with intermittent high glucose and GLP-1 demonstrated a significant increase in proliferation activity (1.45[+ or -]0.12; P
language: eng
source:
identifier: E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2014.2265
fulltext: fulltext
issn:
  • 17913004
  • 1791-3004
url: Link


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titleGLP-1 ameliorates the proliferation activity of INS-1 cells inhibited by intermittent high glucose concentrations through the regulation of cyclins.
creatorZhang, Zhen ; Li, Jing ; Jiang, Xinkui ; Yang, Lei ; Lei, Lei ; Cai, Dehong ; Zhang, Hua ; Chen, Hong
contributorZhang, Zhen (correspondence author) ; Zhang, Zhen (record owner)
ispartofMolecular medicine reports, August 2014, Vol.10(2), pp.683-688
identifierE-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2014.2265
subjectAnimals–Drug Effects ; Cell Line, Tumor–Metabolism ; Cell Proliferation–Metabolism ; Cyclin D1–Metabolism ; Cyclin-Dependent Kinase Inhibitor P21–Metabolism ; Cyclin-Dependent Kinase Inhibitor P27–Drug Effects ; Cyclins–Pharmacology ; G1 Phase Cell Cycle Checkpoints–Pharmacology ; Glucagon-Like Peptide 1–Metabolism ; Glucose–Metabolism ; Rats–Metabolism ; S-Phase Kinase-Associated Proteins–Metabolism ; Cyclin-Dependent Kinase Inhibitor P21 ; Cyclins ; S-Phase Kinase-Associated Proteins ; Cyclin D1 ; Cyclin-Dependent Kinase Inhibitor P27 ; Glucagon-Like Peptide 1 ; Glucose
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descriptionGlucagon-like peptide-1 (GLP-1) and its analog exendin (EX)-4 have been considered to promote [beta]-cell growth and expansion. In the present, study we investigated the effect of GLP-1 on proliferative activity and cell cycle regulation in the pancreatic insulin-secreting [beta]-cell line, INS-1, treated with intermittent high glucose. INS-1 cells were treated with normal glucose (5.5 mmol/l), constant high glucose (30 mmol/l) and intermittent high glucose (rotation/24 h in 5.5 or 30 mmol/l) in the presence or absence of GLP-1 (100 nmol/l) for seven days. Proliferative activity, cell cycle and the expression of cyclin D1, p21, p27 and Skp2 were examined. INS-1 treated with intermittent high glucose and GLP-1 demonstrated a significant increase in proliferation activity (1.45[+ or -]0.12; P<0.01) and decreased cell proportion in G0/G1 phase (49.73[+ or -]4.04%, P<0.01) compared with those without GLP-1. Furthermore, the expression levels of cyclin D1 and Skp2 were increased, while the expression of p27 and p21 were significantly reduced. Similar results were identified in those treated with constant high glucose and GLP-1. These results suggest that GLP-1 may ease the G0/G1 cell cycle arrest of INS-1 cells induced by intermittent high glucose by upregulating the expression of cyclin D1 and Skp2, downregulating the expression of p21 and p27, and finally promoting the cell cycle progression and proliferation activity. Key words: glucagon-like peptide-1, intermittent high glucose, cell proliferation, cell cycle
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titleGLP-1 ameliorates the proliferation activity of INS-1 cells inhibited by intermittent high glucose concentrations through the regulation of cyclins.
authorZhang, Zhen ; Li, Jing ; Jiang, Xinkui ; Yang, Lei ; Lei, Lei ; Cai, Dehong ; Zhang, Hua ; Chen, Hong
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