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Gene Expressing Difference in Sclerotial Formation of Morchella conica

The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obta... Full description

Journal Title: Indian Journal of Microbiology Sep 2014, Vol.54(3), pp.274-283
Main Author: Chen, Li-Jiao
Other Authors: Chai, Hong-Mei , Chen, Wei-Min , Huang, Xing-Qi , Zhao, Yong-Chang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0046-8991 ; E-ISSN: 0973-7715 ; DOI: 10.1007/s12088-014-0445-0
Link: http://search.proquest.com/docview/1544007035/?pq-origsite=primo
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title: Gene Expressing Difference in Sclerotial Formation of Morchella conica
format: Article
creator:
  • Chen, Li-Jiao
  • Chai, Hong-Mei
  • Chen, Wei-Min
  • Huang, Xing-Qi
  • Zhao, Yong-Chang
subjects:
  • Na Super(+)/Ca Super(2+)-Exchanging Atpase
  • Antisense RNA
  • Transcription
  • Permease
  • Gene Expression
  • Rrna
  • Databases
  • Homology
  • Cyclin-Dependent Kinase
  • Lipoproteins
  • Polymerase Chain Reaction
  • Spores
  • Differential Display
  • Phosphodiesterase
  • Genetics & Taxonomy
  • Human Genetics
ispartof: Indian Journal of Microbiology, Sep 2014, Vol.54(3), pp.274-283
description: The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium-calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some...
language: eng
source:
identifier: ISSN: 0046-8991 ; E-ISSN: 0973-7715 ; DOI: 10.1007/s12088-014-0445-0
fulltext: fulltext
issn:
  • 00468991
  • 0046-8991
  • 09737715
  • 0973-7715
url: Link


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titleGene Expressing Difference in Sclerotial Formation of Morchella conica
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ispartofIndian Journal of Microbiology, Sep 2014, Vol.54(3), pp.274-283
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subjectNa Super(+)/Ca Super(2+)-Exchanging Atpase ; Antisense RNA ; Transcription ; Permease ; Gene Expression ; Rrna ; Databases ; Homology ; Cyclin-Dependent Kinase ; Lipoproteins ; Polymerase Chain Reaction ; Spores ; Differential Display ; Phosphodiesterase ; Genetics & Taxonomy ; Human Genetics
descriptionThe difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium-calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some...
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abstractThe difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium-calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some...
doi10.1007/s12088-014-0445-0
urlhttp://search.proquest.com/docview/1544007035/
date2014-09-01