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Direct detection of FoxP3 expression in thymic double-negative CD4-CD8- cells by flow cytometry.

Foxp3 expression is a marker of regulatory T cells (Treg), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(-)CD8(-) T cell precursors in the thymus by flow cytometry. By increasing the number of collected... Full description

Journal Title: Scientific reports July 25, 2014, Vol.4, p.5781
Main Author: Liu, Gang
Other Authors: Li, Zongfang , Wei, Yang , Lin, Yan , Yang, Cengceng , Liu, Tie
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 2045-2322 ; DOI: 10.1038/srep05781
Link: http://search.proquest.com/docview/1548632934/?pq-origsite=primo
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title: Direct detection of FoxP3 expression in thymic double-negative CD4-CD8- cells by flow cytometry.
format: Article
creator:
  • Liu, Gang
  • Li, Zongfang
  • Wei, Yang
  • Lin, Yan
  • Yang, Cengceng
  • Liu, Tie
subjects:
  • Animals–Metabolism
  • Cd4 Antigens–Metabolism
  • Cd8 Antigens–Metabolism
  • Female–Metabolism
  • Flow Cytometry–Metabolism
  • Forkhead Transcription Factors–Immunology
  • Hyaluronan Receptors–Metabolism
  • Interleukin-2 Receptor Alpha Subunit–Cytology
  • Mice–Immunology
  • Mice, Inbred C57bl–Metabolism
  • T-Lymphocyte Subsets–Cytology
  • T-Lymphocytes–Cytology
  • Thymus Gland–Cytology
  • Cd4 Antigens
  • Cd8 Antigens
  • Forkhead Transcription Factors
  • Foxp3 Protein, Mouse
  • Hyaluronan Receptors
  • Interleukin-2 Receptor Alpha Subunit
ispartof: Scientific reports, July 25, 2014, Vol.4, p.5781
description: Foxp3 expression is a marker of regulatory T cells (Treg), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(-)CD8(-) T cell precursors in the thymus by flow cytometry. By increasing the number of collected cells from the conventional 10(4) cells up to more than 10(6) cells during flow cytometry, we found that DN cells exhibited higher Foxp3 expression than double-positive (DP) CD4(+)CD8(+) and single-positive (SP) CD4(+) or CD8(+) (SP) T cells. CD44(+) expression positively correlated with Foxp3 in thymic DN cells. Furthermore, TCR-β(-)CD25(+) DN cells exhibited the highest frequency of Foxp3-expressing cells. Almost all Foxp3(+) cells expressed CD25in DN cells. These results suggest that Foxp3 expression in DN cells can directly be detected by flow cytometry and it was positively corelated with CD25 and CD44 in DN cells.
language: eng
source:
identifier: E-ISSN: 2045-2322 ; DOI: 10.1038/srep05781
fulltext: fulltext
issn:
  • 20452322
  • 2045-2322
url: Link


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titleDirect detection of FoxP3 expression in thymic double-negative CD4-CD8- cells by flow cytometry.
creatorLiu, Gang ; Li, Zongfang ; Wei, Yang ; Lin, Yan ; Yang, Cengceng ; Liu, Tie
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identifierE-ISSN: 2045-2322 ; DOI: 10.1038/srep05781
subjectAnimals–Metabolism ; Cd4 Antigens–Metabolism ; Cd8 Antigens–Metabolism ; Female–Metabolism ; Flow Cytometry–Metabolism ; Forkhead Transcription Factors–Immunology ; Hyaluronan Receptors–Metabolism ; Interleukin-2 Receptor Alpha Subunit–Cytology ; Mice–Immunology ; Mice, Inbred C57bl–Metabolism ; T-Lymphocyte Subsets–Cytology ; T-Lymphocytes–Cytology ; Thymus Gland–Cytology ; Cd4 Antigens ; Cd8 Antigens ; Forkhead Transcription Factors ; Foxp3 Protein, Mouse ; Hyaluronan Receptors ; Interleukin-2 Receptor Alpha Subunit
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descriptionFoxp3 expression is a marker of regulatory T cells (Treg), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(-)CD8(-) T cell precursors in the thymus by flow cytometry. By increasing the number of collected cells from the conventional 10(4) cells up to more than 10(6) cells during flow cytometry, we found that DN cells exhibited higher Foxp3 expression than double-positive (DP) CD4(+)CD8(+) and single-positive (SP) CD4(+) or CD8(+) (SP) T cells. CD44(+) expression positively correlated with Foxp3 in thymic DN cells. Furthermore, TCR-β(-)CD25(+) DN cells exhibited the highest frequency of Foxp3-expressing cells. Almost all Foxp3(+) cells expressed CD25in DN cells. These results suggest that Foxp3 expression in DN cells can directly be detected by flow cytometry and it was positively corelated with CD25 and CD44 in DN cells.
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