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Catalase addition to vitrification solutions maintains goat ovarian preantral follicles stability.

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.rvsc.2014.05.006 Byline: A.A. Carvalho, L.R. Faustino, C.M.G. Silva, S.V. Castro, C.H. Lobo, F.W. Santos, R.R. Santos, C.C. Campello, V. Bordignon, J.R. Figueiredo, A.P.R. Rodrigues Abstract: The aim of this s... Full description

Journal Title: Research in veterinary science August 2014, Vol.97(1), pp.140-147
Main Author: Carvalho, A A
Other Authors: Faustino, L R , Silva, C M G , Castro, S V , Lobo, C H , Santos, F W , Santos, R R , Campello, C C , Bordignon, V , Figueiredo, J R , Rodrigues, A P R
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1532-2661 ; DOI: 1532-2661 ; DOI: 10.1016/j.rvsc.2014.05.006
Link: http://search.proquest.com/docview/1551336060/?pq-origsite=primo
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title: Catalase addition to vitrification solutions maintains goat ovarian preantral follicles stability.
format: Article
creator:
  • Carvalho, A A
  • Faustino, L R
  • Silva, C M G
  • Castro, S V
  • Lobo, C H
  • Santos, F W
  • Santos, R R
  • Campello, C C
  • Bordignon, V
  • Figueiredo, J R
  • Rodrigues, A P R
subjects:
  • Animals–Drug Effects
  • Apoptosis–Pharmacology
  • Catalase–Methods
  • Cryopreservation–Veterinary
  • Female–Metabolism
  • Goats–Cytology
  • Histones–Drug Effects
  • Ovarian Follicle–Metabolism
  • Phosphoproteins–Metabolism
  • Reactive Oxygen Species–Metabolism
  • Vitrification–Metabolism
  • Histones
  • Phosphoproteins
  • Reactive Oxygen Species
  • Catalase
  • Apoptosis
  • Cryopreservation
  • DNA Damage
  • Ovary
  • Ros Levels
ispartof: Research in veterinary science, August 2014, Vol.97(1), pp.140-147
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.rvsc.2014.05.006 Byline: A.A. Carvalho, L.R. Faustino, C.M.G. Silva, S.V. Castro, C.H. Lobo, F.W. Santos, R.R. Santos, C.C. Campello, V. Bordignon, J.R. Figueiredo, A.P.R. Rodrigues Abstract: The aim of this study was to verify whether the addition of catalase (20aIU/mL) at different steps of goat ovarian tissue vitrification affects ROS levels, follicular morphology and viability, stromal cell density, apoptosis and the expression of proteins related to DNA-damage signaling ([gamma]H2AX) and repair (53BP1). Goat ovarian tissues were analyzed fresh (control) or after vitrification: without catalase (VS-/WS-), with catalase in vitrification solutions (VS+/WS-), with catalase in washing solutions (VS-/WS+) or with catalase in both solutions (VS+/WS+). The vitrification without catalase had higher ROS levels than the control. The catalase, regardless the step of addition, maintained ROS levels similar to the control. There were no difference between treatments regarding follicular viability, stromal cell density and detection of [gamma]H2AX and 53BP1. There was no difference in follicular morphology and DNA fragmentation between groups vitrified. In conclusion, catalase addition to vitrification solutions prevents ROS formation in cryopreserved goat ovarian tissues. Article History: Received 2 October 2013; Accepted 16 May 2014
language: eng
source:
identifier: E-ISSN: 1532-2661 ; DOI: 1532-2661 ; DOI: 10.1016/j.rvsc.2014.05.006
fulltext: fulltext
issn:
  • 15322661
  • 1532-2661
url: Link


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titleCatalase addition to vitrification solutions maintains goat ovarian preantral follicles stability.
creatorCarvalho, A A ; Faustino, L R ; Silva, C M G ; Castro, S V ; Lobo, C H ; Santos, F W ; Santos, R R ; Campello, C C ; Bordignon, V ; Figueiredo, J R ; Rodrigues, A P R
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subjectAnimals–Drug Effects ; Apoptosis–Pharmacology ; Catalase–Methods ; Cryopreservation–Veterinary ; Female–Metabolism ; Goats–Cytology ; Histones–Drug Effects ; Ovarian Follicle–Metabolism ; Phosphoproteins–Metabolism ; Reactive Oxygen Species–Metabolism ; Vitrification–Metabolism ; Histones ; Phosphoproteins ; Reactive Oxygen Species ; Catalase ; Apoptosis ; Cryopreservation ; DNA Damage ; Ovary ; Ros Levels
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descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.rvsc.2014.05.006 Byline: A.A. Carvalho, L.R. Faustino, C.M.G. Silva, S.V. Castro, C.H. Lobo, F.W. Santos, R.R. Santos, C.C. Campello, V. Bordignon, J.R. Figueiredo, A.P.R. Rodrigues Abstract: The aim of this study was to verify whether the addition of catalase (20aIU/mL) at different steps of goat ovarian tissue vitrification affects ROS levels, follicular morphology and viability, stromal cell density, apoptosis and the expression of proteins related to DNA-damage signaling ([gamma]H2AX) and repair (53BP1). Goat ovarian tissues were analyzed fresh (control) or after vitrification: without catalase (VS-/WS-), with catalase in vitrification solutions (VS+/WS-), with catalase in washing solutions (VS-/WS+) or with catalase in both solutions (VS+/WS+). The vitrification without catalase had higher ROS levels than the control. The catalase, regardless the step of addition, maintained ROS levels similar to the control. There were no difference between treatments regarding follicular viability, stromal cell density and detection of [gamma]H2AX and 53BP1. There was no difference in follicular morphology and DNA fragmentation between groups vitrified. In conclusion, catalase addition to vitrification solutions prevents ROS formation in cryopreserved goat ovarian tissues. Article History: Received 2 October 2013; Accepted 16 May 2014
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titleCatalase addition to vitrification solutions maintains goat ovarian preantral follicles stability.
authorCarvalho, A A ; Faustino, L R ; Silva, C M G ; Castro, S V ; Lobo, C H ; Santos, F W ; Santos, R R ; Campello, C C ; Bordignon, V ; Figueiredo, J R ; Rodrigues, A P R
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